Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection
Serotonin (5-HT) is a neurotransmitter involved in many biophysiological processes in the brain and in the gastrointestinal tract. Electrochemical methods are commonly used to quantify 5-HT, but their reliability may suffer due to the time-dependent nature of adsorption-limited 5-HT detection, as we...
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MDPI AG
2023-01-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/6/1/6 |
| _version_ | 1797618988649283584 |
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| author | Ashley Augustiny Chapin Jinjing Han Reza Ghodssi |
| author_facet | Ashley Augustiny Chapin Jinjing Han Reza Ghodssi |
| author_sort | Ashley Augustiny Chapin |
| collection | DOAJ |
| description | Serotonin (5-HT) is a neurotransmitter involved in many biophysiological processes in the brain and in the gastrointestinal tract. Electrochemical methods are commonly used to quantify 5-HT, but their reliability may suffer due to the time-dependent nature of adsorption-limited 5-HT detection, as well as electrode fouling over repeated measurements. Mathematical characterization and modeling of adsorption-based electrochemical signal generation would improve reliability of 5-HT measurement. Here, a model was developed to track 5-HT electrode adsorption and resulting current output by combining Langmuir adsorption kinetic equations and adsorption-limited electrochemical equations. 5-HT adsorption binding parameters were experimentally determined at a carbon-nanotube coated Au electrode: K<sub>D</sub> = 7 × 10<sup>−7</sup> M, k<sub>on</sub> = 130 M<sup>−1</sup> s<sup>−1</sup>, k<sub>off</sub> = 9.1 × 10<sup>−5</sup> s<sup>−1</sup>. A computational model of 5-HT adsorption was then constructed, which could effectively predict 5-HT fouling over 50 measurements (R<sup>2</sup> = 0.9947), as well as predict electrode responses over varying concentrations and measurement times. The model aided in optimizing the measurement of 5-HT secreted from a model enterochromaffin cell line—RIN14B—minimizing measurement time. The presented model simplified and improved the characterization of 5-HT detection at the selected electrode. This could be applied to many other adsorption-limited electrochemical analytes and electrode types, contributing to the improvement of application-specific modeling and optimization processes. |
| first_indexed | 2024-03-11T08:20:39Z |
| format | Article |
| id | doaj.art-fbe8cd4f79744eb484aa0f4e9a3d33b1 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-11T08:20:39Z |
| publishDate | 2023-01-01 |
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| series | Methods and Protocols |
| spelling | doaj.art-fbe8cd4f79744eb484aa0f4e9a3d33b12023-11-16T22:26:14ZengMDPI AGMethods and Protocols2409-92792023-01-0161610.3390/mps6010006Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin DetectionAshley Augustiny Chapin0Jinjing Han1Reza Ghodssi2Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USAInstitute for Systems Research, University of Maryland, College Park, MD 20742, USAFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USASerotonin (5-HT) is a neurotransmitter involved in many biophysiological processes in the brain and in the gastrointestinal tract. Electrochemical methods are commonly used to quantify 5-HT, but their reliability may suffer due to the time-dependent nature of adsorption-limited 5-HT detection, as well as electrode fouling over repeated measurements. Mathematical characterization and modeling of adsorption-based electrochemical signal generation would improve reliability of 5-HT measurement. Here, a model was developed to track 5-HT electrode adsorption and resulting current output by combining Langmuir adsorption kinetic equations and adsorption-limited electrochemical equations. 5-HT adsorption binding parameters were experimentally determined at a carbon-nanotube coated Au electrode: K<sub>D</sub> = 7 × 10<sup>−7</sup> M, k<sub>on</sub> = 130 M<sup>−1</sup> s<sup>−1</sup>, k<sub>off</sub> = 9.1 × 10<sup>−5</sup> s<sup>−1</sup>. A computational model of 5-HT adsorption was then constructed, which could effectively predict 5-HT fouling over 50 measurements (R<sup>2</sup> = 0.9947), as well as predict electrode responses over varying concentrations and measurement times. The model aided in optimizing the measurement of 5-HT secreted from a model enterochromaffin cell line—RIN14B—minimizing measurement time. The presented model simplified and improved the characterization of 5-HT detection at the selected electrode. This could be applied to many other adsorption-limited electrochemical analytes and electrode types, contributing to the improvement of application-specific modeling and optimization processes.https://www.mdpi.com/2409-9279/6/1/6electrochemical biosensoradsorptionmodelingneurotransmitter |
| spellingShingle | Ashley Augustiny Chapin Jinjing Han Reza Ghodssi Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection Methods and Protocols electrochemical biosensor adsorption modeling neurotransmitter |
| title | Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection |
| title_full | Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection |
| title_fullStr | Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection |
| title_full_unstemmed | Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection |
| title_short | Adsorption Kinetic Model Predicts and Improves Reliability of Electrochemical Serotonin Detection |
| title_sort | adsorption kinetic model predicts and improves reliability of electrochemical serotonin detection |
| topic | electrochemical biosensor adsorption modeling neurotransmitter |
| url | https://www.mdpi.com/2409-9279/6/1/6 |
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