GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS
In order to assess the genetic purity of common buckwheat variety ‘Darja’ which is the most commonly produced variety of this crop in Bosnia and Herzegovina, 10 SSR markers have been used. Five samples have been collected from different production regions in B&H (Breza, Nisici Plateau, Ustikoli...
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| Format: | Article |
| Language: | English |
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University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2017-12-01
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| Series: | Genetics & Applications |
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| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/39 |
| _version_ | 1811179040391823360 |
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| author | Jasmin Grahić Mirsad Kurtović Silvio Šimon Mirha Đikić Fuad Gaši |
| author_facet | Jasmin Grahić Mirsad Kurtović Silvio Šimon Mirha Đikić Fuad Gaši |
| author_sort | Jasmin Grahić |
| collection | DOAJ |
| description |
In order to assess the genetic purity of common buckwheat variety ‘Darja’ which is the most commonly produced variety of this crop in Bosnia and Herzegovina, 10 SSR markers have been used. Five samples have been collected from different production regions in B&H (Breza, Nisici Plateau, Ustikolina, Bihac and Bosanska Krupa) and compared to the reference ‘Darja’ sample obtained from an ex situ seed collection from Slovenia. Seven out of ten primer pairs used managed to amplify SSR alleles. Analyses of molecular variance (AMOVA) showed a significant differentiation between the reference and all analyzed ‘Darja’ samples. Furthermore, the factorial correspondence analysis revealed a clear differentiation between the reference and ‘Darja’ samples from the most known production regions of common buckwheat in B&H clustering four out of five analyzed samples very close together. The most divergent one among the analyzed samples was the one from Ustikolina. Genetic purity of varieties of all of cross pollinated species produced in Bosnia and Herzegovina is questionable due to the general use of farm-saved seeds.
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| first_indexed | 2024-04-11T06:29:01Z |
| format | Article |
| id | doaj.art-fbed05c955ad447aa98763656eeb9081 |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-11T06:29:01Z |
| publishDate | 2017-12-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-fbed05c955ad447aa98763656eeb90812022-12-22T04:40:17ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2017-12-0112GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERSJasmin Grahić0Mirsad Kurtović1Silvio Šimon2Mirha Đikić3Fuad Gaši4University of Sarajevo, Faculty of Agriculture and Food Sciences, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Agriculture and Food Sciences, Bosnia and HerzegovinaInstitute of Viticulture and Enology, Croatian Center for Agriculture, Food and Rural Affairs, Zagreb, CroatiaUniversity of Sarajevo, Faculty of Agriculture and Food Sciences, Bosnia and HerzegovinaUniversity of Sarajevo, Faculty of Agriculture and Food Sciences, Bosnia and Herzegovina In order to assess the genetic purity of common buckwheat variety ‘Darja’ which is the most commonly produced variety of this crop in Bosnia and Herzegovina, 10 SSR markers have been used. Five samples have been collected from different production regions in B&H (Breza, Nisici Plateau, Ustikolina, Bihac and Bosanska Krupa) and compared to the reference ‘Darja’ sample obtained from an ex situ seed collection from Slovenia. Seven out of ten primer pairs used managed to amplify SSR alleles. Analyses of molecular variance (AMOVA) showed a significant differentiation between the reference and all analyzed ‘Darja’ samples. Furthermore, the factorial correspondence analysis revealed a clear differentiation between the reference and ‘Darja’ samples from the most known production regions of common buckwheat in B&H clustering four out of five analyzed samples very close together. The most divergent one among the analyzed samples was the one from Ustikolina. Genetic purity of varieties of all of cross pollinated species produced in Bosnia and Herzegovina is questionable due to the general use of farm-saved seeds. https://genapp.ba/editions/index.php/journal/article/view/39common buckwheatDarjaSSRgenetic integrityfactorial correspondence analysis |
| spellingShingle | Jasmin Grahić Mirsad Kurtović Silvio Šimon Mirha Đikić Fuad Gaši GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS Genetics & Applications common buckwheat Darja SSR genetic integrity factorial correspondence analysis |
| title | GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS |
| title_full | GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS |
| title_fullStr | GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS |
| title_full_unstemmed | GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS |
| title_short | GENETIC PURITY ASSESSMENT OF COMMON BUCKWHEAT VARIETY ‘DARJA’ WITH THE USE OF SSR MOLECULAR MARKERS |
| title_sort | genetic purity assessment of common buckwheat variety darja with the use of ssr molecular markers |
| topic | common buckwheat Darja SSR genetic integrity factorial correspondence analysis |
| url | https://genapp.ba/editions/index.php/journal/article/view/39 |
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