Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance
This study investigated the degradation characteristics of propylbenzenes (PBZs, including isopropylbenzene and n-propylbenzene), with high leakage risks and ecological hazards, by a newly isolated marine microalga named Rhinomonas reticulata S6A which is a promising candidate for eco-friendly biore...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1171944/full |
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| author | Shuhao Du Shuhao Du Jiali Cui Jiali Cui Fanping Meng Fanping Meng Haiping Li Haiping Li Hongwu Cui Hongwu Cui Yufan Xia Yufan Xia |
| author_facet | Shuhao Du Shuhao Du Jiali Cui Jiali Cui Fanping Meng Fanping Meng Haiping Li Haiping Li Hongwu Cui Hongwu Cui Yufan Xia Yufan Xia |
| author_sort | Shuhao Du |
| collection | DOAJ |
| description | This study investigated the degradation characteristics of propylbenzenes (PBZs, including isopropylbenzene and n-propylbenzene), with high leakage risks and ecological hazards, by a newly isolated marine microalga named Rhinomonas reticulata S6A which is a promising candidate for eco-friendly bioremediation from marine. About 72% and 56% of n-PBZ and i-PBZ can be degraded after 7 days in culture. The acute toxicity of n-PBZ (96h - EC50 = 2.38 mg/L) was higher than that of i-PBZ (96h - EC50 = 3.65 mg/L). The growth inhibition kinetics of this strain were consistent with the Edwards model (R2 = 0.998) and Aiba model (R2 = 0.999). The optimal concentrations for the degradation of n-PBZ and i-PBZ were calculated to be 2.42 mg/L and 2.78 mg/L, respectively. The degradation trends of PBZs conformed to the zero-order kinetic model, and k increased with initial concentrations. The moderate increase in inoculation density could accelerate the degradation of PBZs, with the maximum specific growth rates (r) of 1.116/d (n-PBZ) and 1.230/d (i-PBZ) at the initial inoculation density of 104 cells/mL, while over-inoculation (initial microalgae density more than 105 cells/mL) was not conducive to the degradation of the pollutants. There is not much data on the biodegradation of PBZs in the aquatic environment, so it would be worthwhile to try to apply the new microalgae to explore the fate of PBZs. |
| first_indexed | 2024-04-09T13:57:20Z |
| format | Article |
| id | doaj.art-fbf0227fd8584291a8222f5ab1ec1cb4 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-09T13:57:20Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-fbf0227fd8584291a8222f5ab1ec1cb42023-05-08T04:38:57ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-05-011010.3389/fmars.2023.11719441171944Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performanceShuhao Du0Shuhao Du1Jiali Cui2Jiali Cui3Fanping Meng4Fanping Meng5Haiping Li6Haiping Li7Hongwu Cui8Hongwu Cui9Yufan Xia10Yufan Xia11Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaCollege of Environmental Science and Engineering, Ocean University of China, Qingdao, Shandong, ChinaKey Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaCollege of Environmental Science and Engineering, Ocean University of China, Qingdao, Shandong, ChinaKey Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaCollege of Environmental Science and Engineering, Ocean University of China, Qingdao, Shandong, ChinaKey Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaCollege of Environmental Science and Engineering, Ocean University of China, Qingdao, Shandong, ChinaKey Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, ChinaKey Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, Shandong, ChinaCollege of Environmental Science and Engineering, Ocean University of China, Qingdao, Shandong, ChinaThis study investigated the degradation characteristics of propylbenzenes (PBZs, including isopropylbenzene and n-propylbenzene), with high leakage risks and ecological hazards, by a newly isolated marine microalga named Rhinomonas reticulata S6A which is a promising candidate for eco-friendly bioremediation from marine. About 72% and 56% of n-PBZ and i-PBZ can be degraded after 7 days in culture. The acute toxicity of n-PBZ (96h - EC50 = 2.38 mg/L) was higher than that of i-PBZ (96h - EC50 = 3.65 mg/L). The growth inhibition kinetics of this strain were consistent with the Edwards model (R2 = 0.998) and Aiba model (R2 = 0.999). The optimal concentrations for the degradation of n-PBZ and i-PBZ were calculated to be 2.42 mg/L and 2.78 mg/L, respectively. The degradation trends of PBZs conformed to the zero-order kinetic model, and k increased with initial concentrations. The moderate increase in inoculation density could accelerate the degradation of PBZs, with the maximum specific growth rates (r) of 1.116/d (n-PBZ) and 1.230/d (i-PBZ) at the initial inoculation density of 104 cells/mL, while over-inoculation (initial microalgae density more than 105 cells/mL) was not conducive to the degradation of the pollutants. There is not much data on the biodegradation of PBZs in the aquatic environment, so it would be worthwhile to try to apply the new microalgae to explore the fate of PBZs.https://www.frontiersin.org/articles/10.3389/fmars.2023.1171944/fullpropylbenzenesRhinomonas reticulatabiodegradationaquatic toxicity toxicitykinetic |
| spellingShingle | Shuhao Du Shuhao Du Jiali Cui Jiali Cui Fanping Meng Fanping Meng Haiping Li Haiping Li Hongwu Cui Hongwu Cui Yufan Xia Yufan Xia Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance Frontiers in Marine Science propylbenzenes Rhinomonas reticulata biodegradation aquatic toxicity toxicity kinetic |
| title | Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance |
| title_full | Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance |
| title_fullStr | Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance |
| title_full_unstemmed | Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance |
| title_short | Bioremediation of propylbenzenes by a novel marine microalga Rhinomonas reticulata S6A isolated from Daya Bay: acute toxicity, growth kinetics and biodegradation performance |
| title_sort | bioremediation of propylbenzenes by a novel marine microalga rhinomonas reticulata s6a isolated from daya bay acute toxicity growth kinetics and biodegradation performance |
| topic | propylbenzenes Rhinomonas reticulata biodegradation aquatic toxicity toxicity kinetic |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1171944/full |
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