<i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs
In the present study, we examined the effects of <i>Cissus verticillata</i> leaf extracts (CVE) against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and ischemia-induced neuronal damage in HT22 cells and gerbil hippocampus. Incubation with CVE produced concentr...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1217 |
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| author | Woosuk Kim Hyun Jung Kwon Hyo Young Jung Soon-Sung Lim Beom-Goo Kang Yong-Bok Jo Dong-Sool Yu Soo Young Choi In Koo Hwang Dae Won Kim |
| author_facet | Woosuk Kim Hyun Jung Kwon Hyo Young Jung Soon-Sung Lim Beom-Goo Kang Yong-Bok Jo Dong-Sool Yu Soo Young Choi In Koo Hwang Dae Won Kim |
| author_sort | Woosuk Kim |
| collection | DOAJ |
| description | In the present study, we examined the effects of <i>Cissus verticillata</i> leaf extracts (CVE) against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and ischemia-induced neuronal damage in HT22 cells and gerbil hippocampus. Incubation with CVE produced concentration-dependent toxicity in HT22 cells. Significant cellular toxicity was observed with >75 μg/mL CVE. CVE treatment at 50 μg/mL ameliorated H<sub>2</sub>O<sub>2</sub>-induced reactive oxygen species formation, DNA fragmentation, and cell death in HT22 cells. In addition, incubation with CVE significantly mitigated the increase in Bax and decrease in Bcl-2 induced by H<sub>2</sub>O<sub>2</sub> treatment in HT22 cells. In an in vivo study, the administration of CVE to gerbils significantly decreased ischemia-induced motor activity 1 d after ischemia, as well as neuronal death and microglial activation 4 d after ischemia, respectively. CVE treatment reduced the release of interleukin-1β, interleukin-6, and tumor necrosis factor-α 6 h after ischemia. Furthermore, CVE treatment significantly ameliorated ischemia-induced phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38. These results suggest that CVE has the potential to reduce the neuronal damage induced by oxidative and ischemic stress by reducing the inflammatory responses and phosphorylation of MAPKs, suggesting that CVE could be a functional food to prevent neuronal damage induced by ischemia. |
| first_indexed | 2024-03-10T10:24:00Z |
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| language | English |
| last_indexed | 2024-03-10T10:24:00Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-fbf03f360dbf4340ba1c0b355f43ed6b2023-11-22T00:14:59ZengMDPI AGPlants2223-77472021-06-01106121710.3390/plants10061217<i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKsWoosuk Kim0Hyun Jung Kwon1Hyo Young Jung2Soon-Sung Lim3Beom-Goo Kang4Yong-Bok Jo5Dong-Sool Yu6Soo Young Choi7In Koo Hwang8Dae Won Kim9Department of Biomedical Sciences, and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, KoreaDepartment of Biomedical Sciences, and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, KoreaDepartment of Veterinary Medicine, Institute of Veterinary Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Food Science and Nutrition, Institute of Korean Nutrition, Hallym University, Chuncheon 24252, KoreaDepartment of Biochemistry, College of Medicine, Hallym University, Chuncheon 24252, KoreaDepartment of Convergence Technology, Graduate School of Venture, Hoseo University, Seoul 06724, KoreaDepartment of Venture Management Graduate School of Venture, Hoseo University, Seoul 06724, KoreaDepartment of Biomedical Sciences, and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, KoreaDepartment of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaDepartment of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, KoreaIn the present study, we examined the effects of <i>Cissus verticillata</i> leaf extracts (CVE) against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- and ischemia-induced neuronal damage in HT22 cells and gerbil hippocampus. Incubation with CVE produced concentration-dependent toxicity in HT22 cells. Significant cellular toxicity was observed with >75 μg/mL CVE. CVE treatment at 50 μg/mL ameliorated H<sub>2</sub>O<sub>2</sub>-induced reactive oxygen species formation, DNA fragmentation, and cell death in HT22 cells. In addition, incubation with CVE significantly mitigated the increase in Bax and decrease in Bcl-2 induced by H<sub>2</sub>O<sub>2</sub> treatment in HT22 cells. In an in vivo study, the administration of CVE to gerbils significantly decreased ischemia-induced motor activity 1 d after ischemia, as well as neuronal death and microglial activation 4 d after ischemia, respectively. CVE treatment reduced the release of interleukin-1β, interleukin-6, and tumor necrosis factor-α 6 h after ischemia. Furthermore, CVE treatment significantly ameliorated ischemia-induced phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38. These results suggest that CVE has the potential to reduce the neuronal damage induced by oxidative and ischemic stress by reducing the inflammatory responses and phosphorylation of MAPKs, suggesting that CVE could be a functional food to prevent neuronal damage induced by ischemia.https://www.mdpi.com/2223-7747/10/6/1217<i>Cissus verticillata</i> extractoxidative stressischemiainflammationMAPK |
| spellingShingle | Woosuk Kim Hyun Jung Kwon Hyo Young Jung Soon-Sung Lim Beom-Goo Kang Yong-Bok Jo Dong-Sool Yu Soo Young Choi In Koo Hwang Dae Won Kim <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs Plants <i>Cissus verticillata</i> extract oxidative stress ischemia inflammation MAPK |
| title | <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs |
| title_full | <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs |
| title_fullStr | <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs |
| title_full_unstemmed | <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs |
| title_short | <i>Cissus verticillata</i> Extract Decreases Neuronal Damage Induced by Oxidative Stress in HT22 Cells and Ischemia in Gerbils by Reducing the Inflammation and Phosphorylation of MAPKs |
| title_sort | i cissus verticillata i extract decreases neuronal damage induced by oxidative stress in ht22 cells and ischemia in gerbils by reducing the inflammation and phosphorylation of mapks |
| topic | <i>Cissus verticillata</i> extract oxidative stress ischemia inflammation MAPK |
| url | https://www.mdpi.com/2223-7747/10/6/1217 |
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