Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor
Shear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/10/2079 |
| _version_ | 1797550359680385024 |
|---|---|
| author | Aleksandr Oseev Nikolay Mukhin Céline Elie-Caille Wilfrid Boireau Ralf Lucklum Thomas Lecompte Fabien Remy-Martin Jean-François Manceau Franck Chollet Thérèse Leblois |
| author_facet | Aleksandr Oseev Nikolay Mukhin Céline Elie-Caille Wilfrid Boireau Ralf Lucklum Thomas Lecompte Fabien Remy-Martin Jean-François Manceau Franck Chollet Thérèse Leblois |
| author_sort | Aleksandr Oseev |
| collection | DOAJ |
| description | Shear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells while keeping the same basic assumptions. Thus, the biosensor readouts are still quite often described using ‘mass’ related terminology. This contribution aims to show that assessment of cell deposits with acoustic biosensors requires a deep understanding of the sensor transduction mechanism. More specifically, the cell deposits should be considered as a structured viscoelastic load and the sensor response depends on both material and topological parameters of the deposits. This shifts the paradigm of acoustic biosensor away from the classical mass loading perspective. As a proof of the concept, we recorded QCM frequency shifts caused by blood platelet deposits on a collagen surface under different rheological conditions and observed the final deposit shape with atomic force microscopy (AFM). The results vividly demonstrate that the frequency shift is highly impacted by the platelet topology on the bio-interface. We support our findings with numerical simulations of viscoelastic unstructured and structured loads in liquid. Both experimental and theoretical studies underline the complexity behind the frequency shift interpretation when acoustic biosensing is used with cell deposits. |
| first_indexed | 2024-03-10T15:27:08Z |
| format | Article |
| id | doaj.art-fbf104cefc4145a2815331854fa6c93f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T15:27:08Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-fbf104cefc4145a2815331854fa6c93f2023-11-20T17:57:38ZengMDPI AGNanomaterials2079-49912020-10-011010207910.3390/nano10102079Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic BiosensorAleksandr Oseev0Nikolay Mukhin1Céline Elie-Caille2Wilfrid Boireau3Ralf Lucklum4Thomas Lecompte5Fabien Remy-Martin6Jean-François Manceau7Franck Chollet8Thérèse Leblois9FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceInstitute for Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, 39106 Magdeburg, GermanyFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceInstitute for Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, 39106 Magdeburg, GermanyGeneva Platelet Group, Faculty of Medicine, Geneva University, 1205 Geneva, SwitzerlandFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceFEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, FranceShear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells while keeping the same basic assumptions. Thus, the biosensor readouts are still quite often described using ‘mass’ related terminology. This contribution aims to show that assessment of cell deposits with acoustic biosensors requires a deep understanding of the sensor transduction mechanism. More specifically, the cell deposits should be considered as a structured viscoelastic load and the sensor response depends on both material and topological parameters of the deposits. This shifts the paradigm of acoustic biosensor away from the classical mass loading perspective. As a proof of the concept, we recorded QCM frequency shifts caused by blood platelet deposits on a collagen surface under different rheological conditions and observed the final deposit shape with atomic force microscopy (AFM). The results vividly demonstrate that the frequency shift is highly impacted by the platelet topology on the bio-interface. We support our findings with numerical simulations of viscoelastic unstructured and structured loads in liquid. Both experimental and theoretical studies underline the complexity behind the frequency shift interpretation when acoustic biosensing is used with cell deposits.https://www.mdpi.com/2079-4991/10/10/2079acoustic biosensorQCMplateletcells surface topology |
| spellingShingle | Aleksandr Oseev Nikolay Mukhin Céline Elie-Caille Wilfrid Boireau Ralf Lucklum Thomas Lecompte Fabien Remy-Martin Jean-François Manceau Franck Chollet Thérèse Leblois Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor Nanomaterials acoustic biosensor QCM platelet cells surface topology |
| title | Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor |
| title_full | Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor |
| title_fullStr | Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor |
| title_full_unstemmed | Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor |
| title_short | Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor |
| title_sort | topology challenge for the assessment of living cell deposits with shear bulk acoustic biosensor |
| topic | acoustic biosensor QCM platelet cells surface topology |
| url | https://www.mdpi.com/2079-4991/10/10/2079 |
| work_keys_str_mv | AT aleksandroseev topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT nikolaymukhin topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT celineeliecaille topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT wilfridboireau topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT ralflucklum topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT thomaslecompte topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT fabienremymartin topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT jeanfrancoismanceau topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT franckchollet topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor AT thereseleblois topologychallengefortheassessmentoflivingcelldepositswithshearbulkacousticbiosensor |