Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr
Introduction: Leukemia is a hematologic malignant disease. To date, chemotherapy is the most effective method for leukemia treatment. However, multidrug resistance (MDR) is a major obstacle of leukemia treatment failure. Previous studies have shown that multidrug resistance phenotype is associated w...
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| Format: | Article |
| Language: | English |
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Chaing Mai University
2015-09-01
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| Series: | Journal of Associated Medical Sciences |
| Subjects: | |
| Online Access: | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/59843 |
| _version_ | 1818875003849932800 |
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| author | Aoranit Somno Songyot Anuchpreeda Sawitree Chiampanichayakul |
| author_facet | Aoranit Somno Songyot Anuchpreeda Sawitree Chiampanichayakul |
| author_sort | Aoranit Somno |
| collection | DOAJ |
| description | Introduction: Leukemia is a hematologic malignant disease. To date, chemotherapy is the most effective method for leukemia treatment. However, multidrug resistance (MDR) is a major obstacle of leukemia treatment failure. Previous studies have shown that multidrug resistance phenotype is associated with P-glycoprotein (P-gp) and survivin which is a drug transporter protein and inhibitors of apoptosis proteins (IAPs), respectively. Moreover, overexpressed CD147 has been reported to be involved in the multidrug resistance cell lines. However, the relationship of P-gp, survivin, and CD147 in leukemia with MDR phenotype have not been reported.
Objective: The purpose of this study was to investigate the expressions of P-gp, survivin and CD147 in leukemic cells with MDR phenotype K562/Adr compared to drug sensitive K562 cells.
Materials and methods: The sensitivity of the adriamycin on K562/Adr (drug resistance) and K562 (drug sensitive) cells were determined by MTT assay. Expressions of P-gp, survivin, and CD147 were then investigated by RT-PCR and Western blotting.
Results: Adriamycin showed the cytotoxic effect on K562 cells more than K562/Adr cells with the inhibitory concentration at 50% growth (IC50) values of 2.17±1.02 µM and >10 µM, respectively. P-gp, survivin, and CD147 showed the significant increase in both protein and mRNA levels in K562/Adr cells by Western blot and RT-PCR.
Conclusion: This study is the first report describing the high level expression of P-gp, survivin, and CD147 in leukemia with multidrug resistance phenotype.
Bull Chiang Mai Assoc Med Sci 2015; 48(3): 173-181. Doi: 10.14456/jams.2015.21 |
| first_indexed | 2024-12-19T13:19:35Z |
| format | Article |
| id | doaj.art-fbf1deed38244995b13e86da395474fb |
| institution | Directory Open Access Journal |
| issn | 2539-6056 2539-6056 |
| language | English |
| last_indexed | 2024-12-19T13:19:35Z |
| publishDate | 2015-09-01 |
| publisher | Chaing Mai University |
| record_format | Article |
| series | Journal of Associated Medical Sciences |
| spelling | doaj.art-fbf1deed38244995b13e86da395474fb2022-12-21T20:19:45ZengChaing Mai UniversityJournal of Associated Medical Sciences2539-60562539-60562015-09-0148317317359843Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/AdrAoranit Somno0Songyot Anuchpreeda1Sawitree Chiampanichayakul2Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityIntroduction: Leukemia is a hematologic malignant disease. To date, chemotherapy is the most effective method for leukemia treatment. However, multidrug resistance (MDR) is a major obstacle of leukemia treatment failure. Previous studies have shown that multidrug resistance phenotype is associated with P-glycoprotein (P-gp) and survivin which is a drug transporter protein and inhibitors of apoptosis proteins (IAPs), respectively. Moreover, overexpressed CD147 has been reported to be involved in the multidrug resistance cell lines. However, the relationship of P-gp, survivin, and CD147 in leukemia with MDR phenotype have not been reported. Objective: The purpose of this study was to investigate the expressions of P-gp, survivin and CD147 in leukemic cells with MDR phenotype K562/Adr compared to drug sensitive K562 cells. Materials and methods: The sensitivity of the adriamycin on K562/Adr (drug resistance) and K562 (drug sensitive) cells were determined by MTT assay. Expressions of P-gp, survivin, and CD147 were then investigated by RT-PCR and Western blotting. Results: Adriamycin showed the cytotoxic effect on K562 cells more than K562/Adr cells with the inhibitory concentration at 50% growth (IC50) values of 2.17±1.02 µM and >10 µM, respectively. P-gp, survivin, and CD147 showed the significant increase in both protein and mRNA levels in K562/Adr cells by Western blot and RT-PCR. Conclusion: This study is the first report describing the high level expression of P-gp, survivin, and CD147 in leukemia with multidrug resistance phenotype. Bull Chiang Mai Assoc Med Sci 2015; 48(3): 173-181. Doi: 10.14456/jams.2015.21https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/59843Multidrug resistanceP-glycoproteinsurvivinCD147K562 cell line |
| spellingShingle | Aoranit Somno Songyot Anuchpreeda Sawitree Chiampanichayakul Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr Journal of Associated Medical Sciences Multidrug resistance P-glycoprotein survivin CD147 K562 cell line |
| title | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| title_full | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| title_fullStr | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| title_full_unstemmed | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| title_short | Expressions of P-glycoprotein, survivin and CD147 molecules on multidrug resistance leukemic cell line K562/Adr |
| title_sort | expressions of p glycoprotein survivin and cd147 molecules on multidrug resistance leukemic cell line k562 adr |
| topic | Multidrug resistance P-glycoprotein survivin CD147 K562 cell line |
| url | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/59843 |
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