Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson
Sea buckthorn (<i>Elaeagnus rhamnoides</i> (L.) A. Nelson) is a small tree or bush. It belongs to the Elaeagnaceae family, and has been used for many years in traditional medicine in both Europe and Asia. However, there is no data on the effect of sea buckthorn leaves and twigs on the pr...
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/19/3620 |
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| author | Bartosz Skalski Bogdan Kontek Agata Rolnik Beata Olas Anna Stochmal Jerzy Żuchowski |
| author_facet | Bartosz Skalski Bogdan Kontek Agata Rolnik Beata Olas Anna Stochmal Jerzy Żuchowski |
| author_sort | Bartosz Skalski |
| collection | DOAJ |
| description | Sea buckthorn (<i>Elaeagnus rhamnoides</i> (L.) A. Nelson) is a small tree or bush. It belongs to the Elaeagnaceae family, and has been used for many years in traditional medicine in both Europe and Asia. However, there is no data on the effect of sea buckthorn leaves and twigs on the properties of blood platelets. The aim of the study was to analyze the biological activity of phenolic extracts from leaves and twigs of sea buckthorn in blood platelets in vitro. Two sets of extracts were used: (1) phenolic compounds from twigs and (2) phenolic compounds from leaves. Their biological effects on human blood platelets were studied by blood platelet adhesion, platelet aggregation, arachidonic acid metabolism and the generation of superoxide anion. Cytotoxicity was also evaluated against platelets. The action of extracts from sea buckthorn twigs and leaves was compared to activities of the phenolic extract (a commercial product from the berries of <i>Aronia melanocarpa</i> (Aronox<sup>®</sup>) with antioxidative and antiplatelet properties. This study is the first to demonstrate that extracts from sea buckthorn leaves and twigs are a source of bioactive compounds which may be used for the prophylaxis and treatment of cardiovascular pathologies associated with blood platelet hyperactivity. Both leaf and twig extracts were found to display anti-platelet activity in vitro. Moreover, the twig extract (rich in proanthocyanidins) displayed better anti-platelet potential than the leaf extract or aronia extract. |
| first_indexed | 2024-12-11T12:23:57Z |
| format | Article |
| id | doaj.art-fbf1e474a14f4230a19055eced558f06 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T12:23:57Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fbf1e474a14f4230a19055eced558f062022-12-22T01:07:28ZengMDPI AGMolecules1420-30492019-10-012419362010.3390/molecules24193620molecules24193620Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. NelsonBartosz Skalski0Bogdan Kontek1Agata Rolnik2Beata Olas3Anna Stochmal4Jerzy Żuchowski5Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, PolandDepartment of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandDepartment of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandSea buckthorn (<i>Elaeagnus rhamnoides</i> (L.) A. Nelson) is a small tree or bush. It belongs to the Elaeagnaceae family, and has been used for many years in traditional medicine in both Europe and Asia. However, there is no data on the effect of sea buckthorn leaves and twigs on the properties of blood platelets. The aim of the study was to analyze the biological activity of phenolic extracts from leaves and twigs of sea buckthorn in blood platelets in vitro. Two sets of extracts were used: (1) phenolic compounds from twigs and (2) phenolic compounds from leaves. Their biological effects on human blood platelets were studied by blood platelet adhesion, platelet aggregation, arachidonic acid metabolism and the generation of superoxide anion. Cytotoxicity was also evaluated against platelets. The action of extracts from sea buckthorn twigs and leaves was compared to activities of the phenolic extract (a commercial product from the berries of <i>Aronia melanocarpa</i> (Aronox<sup>®</sup>) with antioxidative and antiplatelet properties. This study is the first to demonstrate that extracts from sea buckthorn leaves and twigs are a source of bioactive compounds which may be used for the prophylaxis and treatment of cardiovascular pathologies associated with blood platelet hyperactivity. Both leaf and twig extracts were found to display anti-platelet activity in vitro. Moreover, the twig extract (rich in proanthocyanidins) displayed better anti-platelet potential than the leaf extract or aronia extract.https://www.mdpi.com/1420-3049/24/19/3620antiplatelet activityadhesionaggregation<i>e. rhamnoides</i>blood platelets |
| spellingShingle | Bartosz Skalski Bogdan Kontek Agata Rolnik Beata Olas Anna Stochmal Jerzy Żuchowski Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson Molecules antiplatelet activity adhesion aggregation <i>e. rhamnoides</i> blood platelets |
| title | Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson |
| title_full | Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson |
| title_fullStr | Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson |
| title_full_unstemmed | Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson |
| title_short | Anti-Platelet Properties of Phenolic Extracts from the Leaves and Twigs of <i>Elaeagnus rhamnoides</i> (L.) A. Nelson |
| title_sort | anti platelet properties of phenolic extracts from the leaves and twigs of i elaeagnus rhamnoides i l a nelson |
| topic | antiplatelet activity adhesion aggregation <i>e. rhamnoides</i> blood platelets |
| url | https://www.mdpi.com/1420-3049/24/19/3620 |
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