Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG
The purpose of the study was to determine the effect of Yersinia pestis EV NIIEG on the process of neutrophil extracellular traps formation in vivo when modeling plague infection and assess their contribution to antiplague protection.Materials and methods. BALB/c mice, which were immunized subcutane...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2021-02-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1407 |
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| author | A. L. Kravtsov A. Yu. Goncharova S. A. Bugorkova Z. L. Devdariani V. A. Kozhevnikov |
| author_facet | A. L. Kravtsov A. Yu. Goncharova S. A. Bugorkova Z. L. Devdariani V. A. Kozhevnikov |
| author_sort | A. L. Kravtsov |
| collection | DOAJ |
| description | The purpose of the study was to determine the effect of Yersinia pestis EV NIIEG on the process of neutrophil extracellular traps formation in vivo when modeling plague infection and assess their contribution to antiplague protection.Materials and methods. BALB/c mice, which were immunized subcutaneously with the Y. pestis EV NIIEG vaccine strain, were used in the study. Animals were infected with a virulent strain Y. pestis 231 at a dose of 20 LD50 (103 CFU). To evaluate the contribution of neutrophil extracellular traps (NETs) to antibacterial protection, an experimental model was used based on fermenting NETs in the abdominal cavity of mice with nuclease. To calculate the number of NETs in peritoneal exudate (PE) fluorescent microscopy was applied. Phagocytic activity of PE cells was determined by flow cytometry. Bactericidal effect of NETs was recorded using bacteriological method.Results and discussion. In pre-immunized mice, the process of NETs formation in response to the reintroduction of plague microbe living cells was 5 times more intense than in intact animals and was accompanied by a significant increase in the killing of Y. pestis cells in PE. The use of micrococcus nuclease in the experiment for fermentation of the NETs, produced in the body of immunized animals, provided evidence of NET participation in conferring anti-infective protection against plague infection. Thus, the established fact of the NET formation in case of Y. pestis infection of mice immunized with Y. pestis EV NIIEG vaccine strain and the influence of this process on the effectiveness of protection against plague is the basis for further clarifying the immunopathogenetic role of neutrophil granulocytes in plague. |
| first_indexed | 2024-03-08T13:41:54Z |
| format | Article |
| id | doaj.art-fbf37d28e534495fb7282de3ff18e8c7 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:54:53Z |
| publishDate | 2021-02-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-fbf37d28e534495fb7282de3ff18e8c72024-04-05T16:47:15ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2021-02-0104707410.21055/0370-1069-2020-4-70-741246Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEGA. L. Kravtsov0A. Yu. Goncharova1S. A. Bugorkova2Z. L. Devdariani3V. A. Kozhevnikov4Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”The purpose of the study was to determine the effect of Yersinia pestis EV NIIEG on the process of neutrophil extracellular traps formation in vivo when modeling plague infection and assess their contribution to antiplague protection.Materials and methods. BALB/c mice, which were immunized subcutaneously with the Y. pestis EV NIIEG vaccine strain, were used in the study. Animals were infected with a virulent strain Y. pestis 231 at a dose of 20 LD50 (103 CFU). To evaluate the contribution of neutrophil extracellular traps (NETs) to antibacterial protection, an experimental model was used based on fermenting NETs in the abdominal cavity of mice with nuclease. To calculate the number of NETs in peritoneal exudate (PE) fluorescent microscopy was applied. Phagocytic activity of PE cells was determined by flow cytometry. Bactericidal effect of NETs was recorded using bacteriological method.Results and discussion. In pre-immunized mice, the process of NETs formation in response to the reintroduction of plague microbe living cells was 5 times more intense than in intact animals and was accompanied by a significant increase in the killing of Y. pestis cells in PE. The use of micrococcus nuclease in the experiment for fermentation of the NETs, produced in the body of immunized animals, provided evidence of NET participation in conferring anti-infective protection against plague infection. Thus, the established fact of the NET formation in case of Y. pestis infection of mice immunized with Y. pestis EV NIIEG vaccine strain and the influence of this process on the effectiveness of protection against plague is the basis for further clarifying the immunopathogenetic role of neutrophil granulocytes in plague.https://journal.microbe.ru/jour/article/view/1407plague infectionvaccine strain yersinia pestis ev niiegneutrophil extracellular trapsbactericidal activity |
| spellingShingle | A. L. Kravtsov A. Yu. Goncharova S. A. Bugorkova Z. L. Devdariani V. A. Kozhevnikov Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG Проблемы особо опасных инфекций plague infection vaccine strain yersinia pestis ev niieg neutrophil extracellular traps bactericidal activity |
| title | Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG |
| title_full | Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG |
| title_fullStr | Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG |
| title_full_unstemmed | Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG |
| title_short | Formation of Neutrophil Extracellular Traps when Modeling Plague Infection in Mice Immunized with Yersinia pestis EV NIIEG |
| title_sort | formation of neutrophil extracellular traps when modeling plague infection in mice immunized with yersinia pestis ev niieg |
| topic | plague infection vaccine strain yersinia pestis ev niieg neutrophil extracellular traps bactericidal activity |
| url | https://journal.microbe.ru/jour/article/view/1407 |
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