Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus
Interleukins (ILs) are a subgroup of secreted cytokines, which are molecules involved in the intercellular regulation of the immune system. In this study, 12 IL homologs were cloned and functionally identified from obscure puffer Takifugu obscurus, and they were termed as ToIL-1β, ToIL-1, ToIL-6, To...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Fish and Shellfish Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667011923000233 |
| _version_ | 1797401657627115520 |
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| author | Ying Huang Peng Luo Fu-Hui Jiang Hui-Ze Gao Li-Fan Cui Zhe Zhao |
| author_facet | Ying Huang Peng Luo Fu-Hui Jiang Hui-Ze Gao Li-Fan Cui Zhe Zhao |
| author_sort | Ying Huang |
| collection | DOAJ |
| description | Interleukins (ILs) are a subgroup of secreted cytokines, which are molecules involved in the intercellular regulation of the immune system. In this study, 12 IL homologs were cloned and functionally identified from obscure puffer Takifugu obscurus, and they were termed as ToIL-1β, ToIL-1, ToIL-6, ToIL-10, ToIL-11, ToIL-12, ToIL-17, ToIL-18, ToIL-20, ToIL-24, ToIL-27, and ToIL-34. Multiple alignment results showed that except for ToIL-24 and ToIL-27, other deduced ToIL proteins shared typical characteristics and structure with other known fish ILs. Phylogenetic analysis revealed that 12 ToILs were evolutionarily closely related to their counterparts in other selected vertebrates. Tissue distribution assay demonstrated that the mRNA transcripts of most ToIL genes were constitutively expressed in all tissues examined, with relatively high expression in immune tissues. Following Vibrio harveyi and Staphylococcus aureus infection, the expression levels of 12 ToILs in the spleen and liver were significantly upregulated, and their response over time varied. Taken together, these data were discussed accordingly with the ToIL expression and the immune response under the different situations tested. The results suggest that the 12 ToIL genes are involved in the antibacterial immune response in T. obscurus. |
| first_indexed | 2024-03-09T02:13:15Z |
| format | Article |
| id | doaj.art-fbf37e6c614b4198a896c357a4db2684 |
| institution | Directory Open Access Journal |
| issn | 2667-0119 |
| language | English |
| last_indexed | 2024-03-09T02:13:15Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fish and Shellfish Immunology Reports |
| spelling | doaj.art-fbf37e6c614b4198a896c357a4db26842023-12-07T05:31:14ZengElsevierFish and Shellfish Immunology Reports2667-01192023-12-015100103Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurusYing Huang0Peng Luo1Fu-Hui Jiang2Hui-Ze Gao3Li-Fan Cui4Zhe Zhao5Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510301, ChinaJiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization; Department of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China; Corresponding author.Interleukins (ILs) are a subgroup of secreted cytokines, which are molecules involved in the intercellular regulation of the immune system. In this study, 12 IL homologs were cloned and functionally identified from obscure puffer Takifugu obscurus, and they were termed as ToIL-1β, ToIL-1, ToIL-6, ToIL-10, ToIL-11, ToIL-12, ToIL-17, ToIL-18, ToIL-20, ToIL-24, ToIL-27, and ToIL-34. Multiple alignment results showed that except for ToIL-24 and ToIL-27, other deduced ToIL proteins shared typical characteristics and structure with other known fish ILs. Phylogenetic analysis revealed that 12 ToILs were evolutionarily closely related to their counterparts in other selected vertebrates. Tissue distribution assay demonstrated that the mRNA transcripts of most ToIL genes were constitutively expressed in all tissues examined, with relatively high expression in immune tissues. Following Vibrio harveyi and Staphylococcus aureus infection, the expression levels of 12 ToILs in the spleen and liver were significantly upregulated, and their response over time varied. Taken together, these data were discussed accordingly with the ToIL expression and the immune response under the different situations tested. The results suggest that the 12 ToIL genes are involved in the antibacterial immune response in T. obscurus.http://www.sciencedirect.com/science/article/pii/S2667011923000233InterleukinsExpression pattern analysisAntibacterial immunityTakifugu obscurus |
| spellingShingle | Ying Huang Peng Luo Fu-Hui Jiang Hui-Ze Gao Li-Fan Cui Zhe Zhao Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus Fish and Shellfish Immunology Reports Interleukins Expression pattern analysis Antibacterial immunity Takifugu obscurus |
| title | Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus |
| title_full | Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus |
| title_fullStr | Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus |
| title_full_unstemmed | Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus |
| title_short | Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus |
| title_sort | molecular cloning characterization and gene expression analysis of twelve interleukins in obscure puffer takifugu obscurus |
| topic | Interleukins Expression pattern analysis Antibacterial immunity Takifugu obscurus |
| url | http://www.sciencedirect.com/science/article/pii/S2667011923000233 |
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