Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study
Abstract Background Ubiquitously transcribed tetratricopeptide repeat on chromosome X (UTX) has been identified as a histone 3 lysine 27 (H3K27) demethylase and acted as a tumor suppressor gene or oncogenic function. The current study was to explore the significance of UTX in oral tongue squamous ce...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-021-08726-3 |
| _version_ | 1818449418228072448 |
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| author | Yen-Hao Chen Chang-Han Chen Chih-Yen Chien Yan-Ye Su Sheng-Dean Luo Shau-Hsuan Li |
| author_facet | Yen-Hao Chen Chang-Han Chen Chih-Yen Chien Yan-Ye Su Sheng-Dean Luo Shau-Hsuan Li |
| author_sort | Yen-Hao Chen |
| collection | DOAJ |
| description | Abstract Background Ubiquitously transcribed tetratricopeptide repeat on chromosome X (UTX) has been identified as a histone 3 lysine 27 (H3K27) demethylase and acted as a tumor suppressor gene or oncogenic function. The current study was to explore the significance of UTX in oral tongue squamous cell carcinoma (OTSCC) patients who received surgical resection. Methods A total of 148 OTSCC patients who underwent surgical resection were identified, including 64 patients (43%) with overexpression of UTX and 84 patients (57%) harboring low expression of UTX. We also used two OTSCC cell lines, SAS and Cal 27, to determine the modulation of cancer. Chi-square test was used to investigate the difference of categorical variables between the groups; survival outcome was analyzed using the Kaplan–Meier method in univariate analysis, and a Cox regression model was performed for multivariate analyses. Results Univariate and multivariate analyses showed overexpression of UTX were significantly related to worse disease-free survival (P = 0.028) and overall survival (P = 0.029). The two OTSCC cell lines were treated with GSK-J4, a potent inhibitor of UTX, and transwell migration and invasion assays showed an inhibitory effect with a dose-dependent manner. In addition, western blot analyses also revealed the inhibition of cell cycle and epithelial-mesenchymal transition. Conclusion Our study suggests that UTX plays an important role in the process of OTSCC and overexpression of UTX may predict poor prognosis in OTSCC patients who received surgical resection. |
| first_indexed | 2024-12-14T20:35:05Z |
| format | Article |
| id | doaj.art-fbfdae408c4b45c08f4bcb0551f0e8e0 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-14T20:35:05Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-fbfdae408c4b45c08f4bcb0551f0e8e02022-12-21T22:48:26ZengBMCBMC Cancer1471-24072021-09-0121111410.1186/s12885-021-08726-3Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control studyYen-Hao Chen0Chang-Han Chen1Chih-Yen Chien2Yan-Ye Su3Sheng-Dean Luo4Shau-Hsuan Li5Department of Hematology-Oncology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineInstitute of Medicine, Chung Shan Medical University, Department of Medical Research, Chung Shan Medical University HospitalDepartment of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Hematology-Oncology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineAbstract Background Ubiquitously transcribed tetratricopeptide repeat on chromosome X (UTX) has been identified as a histone 3 lysine 27 (H3K27) demethylase and acted as a tumor suppressor gene or oncogenic function. The current study was to explore the significance of UTX in oral tongue squamous cell carcinoma (OTSCC) patients who received surgical resection. Methods A total of 148 OTSCC patients who underwent surgical resection were identified, including 64 patients (43%) with overexpression of UTX and 84 patients (57%) harboring low expression of UTX. We also used two OTSCC cell lines, SAS and Cal 27, to determine the modulation of cancer. Chi-square test was used to investigate the difference of categorical variables between the groups; survival outcome was analyzed using the Kaplan–Meier method in univariate analysis, and a Cox regression model was performed for multivariate analyses. Results Univariate and multivariate analyses showed overexpression of UTX were significantly related to worse disease-free survival (P = 0.028) and overall survival (P = 0.029). The two OTSCC cell lines were treated with GSK-J4, a potent inhibitor of UTX, and transwell migration and invasion assays showed an inhibitory effect with a dose-dependent manner. In addition, western blot analyses also revealed the inhibition of cell cycle and epithelial-mesenchymal transition. Conclusion Our study suggests that UTX plays an important role in the process of OTSCC and overexpression of UTX may predict poor prognosis in OTSCC patients who received surgical resection.https://doi.org/10.1186/s12885-021-08726-3UTXTongue cancerSquamous cell carcinomaSurgery |
| spellingShingle | Yen-Hao Chen Chang-Han Chen Chih-Yen Chien Yan-Ye Su Sheng-Dean Luo Shau-Hsuan Li Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study BMC Cancer UTX Tongue cancer Squamous cell carcinoma Surgery |
| title | Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study |
| title_full | Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study |
| title_fullStr | Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study |
| title_full_unstemmed | Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study |
| title_short | Overexpression of UTX promotes tumor progression in Oral tongue squamous cell carcinoma patients receiving surgical resection: a case control study |
| title_sort | overexpression of utx promotes tumor progression in oral tongue squamous cell carcinoma patients receiving surgical resection a case control study |
| topic | UTX Tongue cancer Squamous cell carcinoma Surgery |
| url | https://doi.org/10.1186/s12885-021-08726-3 |
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