L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells
L-theanine (LTA) is a key component in tea plants and is commonly used as a functional ingredient and dietary supplement. In a recent study, we investigated the potential antiaging properties of LTA using a D-galactose-induced L6 skeletal muscle aging cell model. The results showed that LTA treatmen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-05-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624001373 |
| _version_ | 1827255818013114368 |
|---|---|
| author | Min Long Qinyu Zhou Xi Xiang Kehong Liu Wenjun Xiao |
| author_facet | Min Long Qinyu Zhou Xi Xiang Kehong Liu Wenjun Xiao |
| author_sort | Min Long |
| collection | DOAJ |
| description | L-theanine (LTA) is a key component in tea plants and is commonly used as a functional ingredient and dietary supplement. In a recent study, we investigated the potential antiaging properties of LTA using a D-galactose-induced L6 skeletal muscle aging cell model. The results showed that LTA treatment improved cell viability and antioxidant enzyme activities while reducing levels of proinflammatory factors and advanced glycation end products. Additionally, LTA demonstrated the ability to decrease senescence-associated beta-galactosidase-positive cells, lower reactive oxygen species and MDA levels, preserve mitochondrial function, inhibit apoptosis, and alleviate cell cycle arrest in the G2/M phase. These protective effects may be attributed to the activation of the p53/p21/CDK4 pathway, regulation of cell cycle-related protein expression, promotion of cell proliferation, and regulation of apoptosis-related protein expression through the p53/Bax/Bcl-2 pathway. |
| first_indexed | 2024-04-24T23:19:01Z |
| format | Article |
| id | doaj.art-fbfdb1b87b1c4b1a911d4af612de748e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2025-03-22T01:29:03Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-fbfdb1b87b1c4b1a911d4af612de748e2024-05-10T04:33:41ZengElsevierJournal of Functional Foods1756-46462024-05-01116106135L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cellsMin Long0Qinyu Zhou1Xi Xiang2Kehong Liu3Wenjun Xiao4Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; Guyanghe Tea Industry Co., Ltd. in Guzhang County, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province, Guzhang, Hunan 416300, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, ChinaKey Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, ChinaKey Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, ChinaKey Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China; Corresponding authors at: Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China.Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China; National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China; Corresponding authors at: Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China.L-theanine (LTA) is a key component in tea plants and is commonly used as a functional ingredient and dietary supplement. In a recent study, we investigated the potential antiaging properties of LTA using a D-galactose-induced L6 skeletal muscle aging cell model. The results showed that LTA treatment improved cell viability and antioxidant enzyme activities while reducing levels of proinflammatory factors and advanced glycation end products. Additionally, LTA demonstrated the ability to decrease senescence-associated beta-galactosidase-positive cells, lower reactive oxygen species and MDA levels, preserve mitochondrial function, inhibit apoptosis, and alleviate cell cycle arrest in the G2/M phase. These protective effects may be attributed to the activation of the p53/p21/CDK4 pathway, regulation of cell cycle-related protein expression, promotion of cell proliferation, and regulation of apoptosis-related protein expression through the p53/Bax/Bcl-2 pathway.http://www.sciencedirect.com/science/article/pii/S1756464624001373L-theanineAgingL6 cellp53/p21/CDK4p53/Bax/Bcl-2 |
| spellingShingle | Min Long Qinyu Zhou Xi Xiang Kehong Liu Wenjun Xiao L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells Journal of Functional Foods L-theanine Aging L6 cell p53/p21/CDK4 p53/Bax/Bcl-2 |
| title | L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells |
| title_full | L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells |
| title_fullStr | L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells |
| title_full_unstemmed | L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells |
| title_short | L-theanine protects against D-galactose-induced aging in L6 skeletal muscle cells |
| title_sort | l theanine protects against d galactose induced aging in l6 skeletal muscle cells |
| topic | L-theanine Aging L6 cell p53/p21/CDK4 p53/Bax/Bcl-2 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624001373 |
| work_keys_str_mv | AT minlong ltheanineprotectsagainstdgalactoseinducedaginginl6skeletalmusclecells AT qinyuzhou ltheanineprotectsagainstdgalactoseinducedaginginl6skeletalmusclecells AT xixiang ltheanineprotectsagainstdgalactoseinducedaginginl6skeletalmusclecells AT kehongliu ltheanineprotectsagainstdgalactoseinducedaginginl6skeletalmusclecells AT wenjunxiao ltheanineprotectsagainstdgalactoseinducedaginginl6skeletalmusclecells |