Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model
The lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous st...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520341584 |
| _version_ | 1818384173467959296 |
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| author | Charlotte M. Beddoes Gert S. Gooris Joke A. Bouwstra |
| author_facet | Charlotte M. Beddoes Gert S. Gooris Joke A. Bouwstra |
| author_sort | Charlotte M. Beddoes |
| collection | DOAJ |
| description | The lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous studies of LPP lipid models have identified the position of the major lipid classes and suggested that a large fraction of FFAs and the ceramide acyl chain are present in the central region. However, the precise arrangement, such as lipid subclass mixing (isolated or mixed) and ceramide conformation (extended or hairpin), remains unknown. Here, we deuterated FFAs and the ceramide acyl chain to study CD2 and CH2 interactions with Fourier-transform infrared spectroscopy. The ceramide and FFAs of various chain lengths were not in separate domains but had mixed together. The larger number of CD2-CD2 lipid chain interactions in the LPP than in a symmetrical bilayer structure implied that the ceramide had primarily adopted an extended conformation. Shorter FFAs were present in the central region of the LPP. This model explores the biophysical properties of the stratum corneum's LPP to improve the understanding of the barrier function of this layer. |
| first_indexed | 2024-12-14T03:18:03Z |
| format | Article |
| id | doaj.art-fc00215286304b268215e274d91fb6fb |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:18:03Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fc00215286304b268215e274d91fb6fb2022-12-21T23:19:06ZengElsevierJournal of Lipid Research0022-22752018-12-01591223292338Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix modelCharlotte M. Beddoes0Gert S. Gooris1Joke A. Bouwstra2Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The NetherlandsLeiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The NetherlandsTo whom correspondence should be addressed.; To whom correspondence should be addressed.; Leiden Academic Centre for Drug Research, Division of BioTherapeutics, Gorlaeus Laboratories, University of Leiden, Leiden, The NetherlandsThe lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous studies of LPP lipid models have identified the position of the major lipid classes and suggested that a large fraction of FFAs and the ceramide acyl chain are present in the central region. However, the precise arrangement, such as lipid subclass mixing (isolated or mixed) and ceramide conformation (extended or hairpin), remains unknown. Here, we deuterated FFAs and the ceramide acyl chain to study CD2 and CH2 interactions with Fourier-transform infrared spectroscopy. The ceramide and FFAs of various chain lengths were not in separate domains but had mixed together. The larger number of CD2-CD2 lipid chain interactions in the LPP than in a symmetrical bilayer structure implied that the ceramide had primarily adopted an extended conformation. Shorter FFAs were present in the central region of the LPP. This model explores the biophysical properties of the stratum corneum's LPP to improve the understanding of the barrier function of this layer.http://www.sciencedirect.com/science/article/pii/S0022227520341584skinceramidefatty acidsmembranesphysical chemistryphysical biochemistry |
| spellingShingle | Charlotte M. Beddoes Gert S. Gooris Joke A. Bouwstra Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model Journal of Lipid Research skin ceramide fatty acids membranes physical chemistry physical biochemistry |
| title | Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model |
| title_full | Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model |
| title_fullStr | Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model |
| title_full_unstemmed | Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model |
| title_short | Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model |
| title_sort | preferential arrangement of lipids in the long periodicity phase of a stratum corneum matrix model |
| topic | skin ceramide fatty acids membranes physical chemistry physical biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520341584 |
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