High-resolution visualization of pial surface vessels by flattened whole mount staining
Summary: Understanding development of the cerebral vasculature is essential for the central nervous system (CNS) research and therapeutic developments. Here, we developed a simple, convenient, and fast method—the flattened cortex whole mount (FCWM) technique—for imaging of pial cerebral vessels. FCW...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223005448 |
| _version_ | 1797854371326722048 |
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| author | Yanying Xu Jiasheng Zhang Heon-Woo Lee Guogang Zhang Yongping Bai Michael Simons |
| author_facet | Yanying Xu Jiasheng Zhang Heon-Woo Lee Guogang Zhang Yongping Bai Michael Simons |
| author_sort | Yanying Xu |
| collection | DOAJ |
| description | Summary: Understanding development of the cerebral vasculature is essential for the central nervous system (CNS) research and therapeutic developments. Here, we developed a simple, convenient, and fast method—the flattened cortex whole mount (FCWM) technique—for imaging of pial cerebral vessels. FCWM involves dissection of the whole cerebral cortex followed by flattening, sectioning and application of CLARITY technology. Compared to conventional methods, FCWM offers several advantages including (1) high-resolution visualization of the whole cortex pial surface vessel structures and distributions; (2) precise localization of a particular blood vessel, allowing observations of a desired blood vessel during normal development or in disease settings; (3) compatibility with confocal imaging. Application of FCWM for examination of cerebral vasculature during postnatal development or in stroke settings allowed us to demonstrate that cerebral blood vessels manifest type-specific maturation and remodeling which are linked to the rate of endothelial proliferation. |
| first_indexed | 2024-04-09T20:04:53Z |
| format | Article |
| id | doaj.art-fc0228d9759343c1b1400ba919e91efa |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T20:04:53Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-fc0228d9759343c1b1400ba919e91efa2023-04-02T06:14:14ZengElsevieriScience2589-00422023-04-01264106467High-resolution visualization of pial surface vessels by flattened whole mount stainingYanying Xu0Jiasheng Zhang1Heon-Woo Lee2Guogang Zhang3Yongping Bai4Michael Simons5Yale Cardiovascular Research Center, Yale University School of Medicine, New Haven, CT, USA; Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaYale Cardiovascular Research Center, Yale University School of Medicine, New Haven, CT, USAYale Cardiovascular Research Center, Yale University School of Medicine, New Haven, CT, USADepartment of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Geriatric Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. ChinaYale Cardiovascular Research Center, Yale University School of Medicine, New Haven, CT, USA; Corresponding authorSummary: Understanding development of the cerebral vasculature is essential for the central nervous system (CNS) research and therapeutic developments. Here, we developed a simple, convenient, and fast method—the flattened cortex whole mount (FCWM) technique—for imaging of pial cerebral vessels. FCWM involves dissection of the whole cerebral cortex followed by flattening, sectioning and application of CLARITY technology. Compared to conventional methods, FCWM offers several advantages including (1) high-resolution visualization of the whole cortex pial surface vessel structures and distributions; (2) precise localization of a particular blood vessel, allowing observations of a desired blood vessel during normal development or in disease settings; (3) compatibility with confocal imaging. Application of FCWM for examination of cerebral vasculature during postnatal development or in stroke settings allowed us to demonstrate that cerebral blood vessels manifest type-specific maturation and remodeling which are linked to the rate of endothelial proliferation.http://www.sciencedirect.com/science/article/pii/S2589004223005448Vascular anatomyMedical imagingNeuroscience |
| spellingShingle | Yanying Xu Jiasheng Zhang Heon-Woo Lee Guogang Zhang Yongping Bai Michael Simons High-resolution visualization of pial surface vessels by flattened whole mount staining iScience Vascular anatomy Medical imaging Neuroscience |
| title | High-resolution visualization of pial surface vessels by flattened whole mount staining |
| title_full | High-resolution visualization of pial surface vessels by flattened whole mount staining |
| title_fullStr | High-resolution visualization of pial surface vessels by flattened whole mount staining |
| title_full_unstemmed | High-resolution visualization of pial surface vessels by flattened whole mount staining |
| title_short | High-resolution visualization of pial surface vessels by flattened whole mount staining |
| title_sort | high resolution visualization of pial surface vessels by flattened whole mount staining |
| topic | Vascular anatomy Medical imaging Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223005448 |
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