Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production
IntroductionClostridium tyrobutyricum has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for C. tyrobutyricum fermentation, but...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1065953/full |
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| author | Bing Wang Bing Wang Xiang Zhou Xiang Zhou Wei Liu Mei-Han Liu Mei-Han Liu Dan Mo Dan Mo Qing-Feng Wu Ya-Juan Wang Ya-Juan Wang Miao-Miao Zhang Lei Chen Shan Yuan Bo Zhou Xin Li Dong Lu Dong Lu Dong Lu |
| author_facet | Bing Wang Bing Wang Xiang Zhou Xiang Zhou Wei Liu Mei-Han Liu Mei-Han Liu Dan Mo Dan Mo Qing-Feng Wu Ya-Juan Wang Ya-Juan Wang Miao-Miao Zhang Lei Chen Shan Yuan Bo Zhou Xin Li Dong Lu Dong Lu Dong Lu |
| author_sort | Bing Wang |
| collection | DOAJ |
| description | IntroductionClostridium tyrobutyricum has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for C. tyrobutyricum fermentation, but there is no suitable method available at present.MethodsIn this study, an acid-base treatment combined with a new green membrane treatment technology – a dynamic ion-exchange membrane -was used to pretreat refinery final molasses, so that it could be used for C. tyrobutyricum to produce butyric acid. A high-performance liquid chromatography method was established to determine the conversion of a large amount of sucrose into fermentable sugars (71.88 g/L glucose and 38.06 g/L fructose) in the treated refinery final molasses. The process of sequential filtration with 3, 1, and 0.45 μm-pore diameter dynamic ion-exchange membranes could remove impurities, pigments, and harmful substances from the refinery final molasses, and retain the fermentable sugar.Results and discussionThis means that refinery final molasses from the sugar industry could be utilized as a high-value by-product and used for the growth of C. tyrobutyricum, with industrial feasibility and economic competitiveness. Using the treated refinery final molasses as a carbon source, C. tyrobutyricum was screened by the method of adaptive evolution. The strain with butyric acid yielded 52.54 g/L, and the yield of the six carbon sugar was increased from 0.240 to 0.478 g/g. The results showed that combination of C. tyrobutyricum and ionic membrane technology broke through the bottleneck of its utilization of refinery final molasses. This study provided an innovative idea for the C. tyrobutyricum fermentation to produce butyric acid. |
| first_indexed | 2024-04-10T16:56:58Z |
| format | Article |
| id | doaj.art-fc02a163d4574877b653785171494299 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T16:56:58Z |
| publishDate | 2023-02-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-fc02a163d4574877b6537851714942992023-02-07T07:13:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.10659531065953Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid productionBing Wang0Bing Wang1Xiang Zhou2Xiang Zhou3Wei Liu4Mei-Han Liu5Mei-Han Liu6Dan Mo7Dan Mo8Qing-Feng Wu9Ya-Juan Wang10Ya-Juan Wang11Miao-Miao Zhang12Lei Chen13Shan Yuan14Bo Zhou15Xin Li16Dong Lu17Dong Lu18Dong Lu19Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaCollege of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaCollege of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, ChinaCAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, ChinaCollege of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, ChinaCollege of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, ChinaInstitute of Modern Physics, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, ChinaGansu Key Laboratory of Microbial Resources Exploitation and Application, Lanzhou, ChinaIntroductionClostridium tyrobutyricum has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for C. tyrobutyricum fermentation, but there is no suitable method available at present.MethodsIn this study, an acid-base treatment combined with a new green membrane treatment technology – a dynamic ion-exchange membrane -was used to pretreat refinery final molasses, so that it could be used for C. tyrobutyricum to produce butyric acid. A high-performance liquid chromatography method was established to determine the conversion of a large amount of sucrose into fermentable sugars (71.88 g/L glucose and 38.06 g/L fructose) in the treated refinery final molasses. The process of sequential filtration with 3, 1, and 0.45 μm-pore diameter dynamic ion-exchange membranes could remove impurities, pigments, and harmful substances from the refinery final molasses, and retain the fermentable sugar.Results and discussionThis means that refinery final molasses from the sugar industry could be utilized as a high-value by-product and used for the growth of C. tyrobutyricum, with industrial feasibility and economic competitiveness. Using the treated refinery final molasses as a carbon source, C. tyrobutyricum was screened by the method of adaptive evolution. The strain with butyric acid yielded 52.54 g/L, and the yield of the six carbon sugar was increased from 0.240 to 0.478 g/g. The results showed that combination of C. tyrobutyricum and ionic membrane technology broke through the bottleneck of its utilization of refinery final molasses. This study provided an innovative idea for the C. tyrobutyricum fermentation to produce butyric acid.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1065953/fullClostridium tyrobutyricumrefinery final molassesionic membraneadaptive evolutionbutyric acid |
| spellingShingle | Bing Wang Bing Wang Xiang Zhou Xiang Zhou Wei Liu Mei-Han Liu Mei-Han Liu Dan Mo Dan Mo Qing-Feng Wu Ya-Juan Wang Ya-Juan Wang Miao-Miao Zhang Lei Chen Shan Yuan Bo Zhou Xin Li Dong Lu Dong Lu Dong Lu Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production Frontiers in Microbiology Clostridium tyrobutyricum refinery final molasses ionic membrane adaptive evolution butyric acid |
| title | Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| title_full | Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| title_fullStr | Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| title_full_unstemmed | Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| title_short | Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| title_sort | construction of clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production |
| topic | Clostridium tyrobutyricum refinery final molasses ionic membrane adaptive evolution butyric acid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1065953/full |
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