Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment

A total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent...

Full description

Bibliographic Details
Main Authors: Ghulam Fareed, Sohail Hassan Khan, Muhammad Ashraf Anjum, Naveed Ahmed
Format: Article
Language:English
Published: Network for the Veterinarians of Bangladesh 2014-12-01
Series:Journal of Advanced Veterinary and Animal Research
Subjects:
Online Access:http://www.scopemed.org/fulltextpdf.php?mno=165557
_version_ 1819027244490686464
author Ghulam Fareed
Sohail Hassan Khan
Muhammad Ashraf Anjum
Naveed Ahmed
author_facet Ghulam Fareed
Sohail Hassan Khan
Muhammad Ashraf Anjum
Naveed Ahmed
author_sort Ghulam Fareed
collection DOAJ
description A total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent Assay (ELISA). Overall incidence of TA was recorded as 80.64% (n=150/186); whereas, in the feed ingredients, it was 86.84% (n=99/114), and in the finished feeds, the incidence of TA was 70.83% (n=51/72). Corn, cotton seed meal, sunflower meal, and cotton gluten meal were found to be highly (100%) contaminated with TA. The OTA was determined in 63.15% (n=72/114) and 29.17% (n=21/72) feed ingredients, and finished feed samples, respectively, with an overall incidence of 50% (n=93/186). Maximum level of OTA contamination (100%) was recorded in corn gluten meal. However, no feed contained OTA above the acceptable level as set by the European Union on OTA contamination in poultry finished feed. On the other hand, a number of samples contained TA above the acceptable limit. Thus, immediate control measures should be taken to ensure safe poultry for human consumption.
first_indexed 2024-12-21T05:39:23Z
format Article
id doaj.art-fc0568ac7bd4411ebc8bad3185152ea1
institution Directory Open Access Journal
issn 2311-7710
2311-7710
language English
last_indexed 2024-12-21T05:39:23Z
publishDate 2014-12-01
publisher Network for the Veterinarians of Bangladesh
record_format Article
series Journal of Advanced Veterinary and Animal Research
spelling doaj.art-fc0568ac7bd4411ebc8bad3185152ea12022-12-21T19:14:19ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102311-77102014-12-011420120710.5455/javar.2014.a38Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environmentGhulam Fareed0Sohail Hassan Khan1Muhammad Ashraf Anjum2Naveed Ahmed3Poultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanA total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent Assay (ELISA). Overall incidence of TA was recorded as 80.64% (n=150/186); whereas, in the feed ingredients, it was 86.84% (n=99/114), and in the finished feeds, the incidence of TA was 70.83% (n=51/72). Corn, cotton seed meal, sunflower meal, and cotton gluten meal were found to be highly (100%) contaminated with TA. The OTA was determined in 63.15% (n=72/114) and 29.17% (n=21/72) feed ingredients, and finished feed samples, respectively, with an overall incidence of 50% (n=93/186). Maximum level of OTA contamination (100%) was recorded in corn gluten meal. However, no feed contained OTA above the acceptable level as set by the European Union on OTA contamination in poultry finished feed. On the other hand, a number of samples contained TA above the acceptable limit. Thus, immediate control measures should be taken to ensure safe poultry for human consumption.http://www.scopemed.org/fulltextpdf.php?mno=165557AflatoxinMoistureOchratoxinPoultry feedPoultry feed ingredients
spellingShingle Ghulam Fareed
Sohail Hassan Khan
Muhammad Ashraf Anjum
Naveed Ahmed
Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
Journal of Advanced Veterinary and Animal Research
Aflatoxin
Moisture
Ochratoxin
Poultry feed
Poultry feed ingredients
title Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
title_full Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
title_fullStr Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
title_full_unstemmed Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
title_short Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
title_sort determination of aflatoxin and ochratoxin in poultry feed ingredients and finished feed in humid semi tropical environment
topic Aflatoxin
Moisture
Ochratoxin
Poultry feed
Poultry feed ingredients
url http://www.scopemed.org/fulltextpdf.php?mno=165557
work_keys_str_mv AT ghulamfareed determinationofaflatoxinandochratoxininpoultryfeedingredientsandfinishedfeedinhumidsemitropicalenvironment
AT sohailhassankhan determinationofaflatoxinandochratoxininpoultryfeedingredientsandfinishedfeedinhumidsemitropicalenvironment
AT muhammadashrafanjum determinationofaflatoxinandochratoxininpoultryfeedingredientsandfinishedfeedinhumidsemitropicalenvironment
AT naveedahmed determinationofaflatoxinandochratoxininpoultryfeedingredientsandfinishedfeedinhumidsemitropicalenvironment