Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment
A total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent...
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Format: | Article |
Language: | English |
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Network for the Veterinarians of Bangladesh
2014-12-01
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Series: | Journal of Advanced Veterinary and Animal Research |
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Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=165557 |
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author | Ghulam Fareed Sohail Hassan Khan Muhammad Ashraf Anjum Naveed Ahmed |
author_facet | Ghulam Fareed Sohail Hassan Khan Muhammad Ashraf Anjum Naveed Ahmed |
author_sort | Ghulam Fareed |
collection | DOAJ |
description | A total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent Assay (ELISA). Overall incidence of TA was recorded as 80.64% (n=150/186); whereas, in the feed ingredients, it was 86.84% (n=99/114), and in the finished feeds, the incidence of TA was 70.83% (n=51/72). Corn, cotton seed meal, sunflower meal, and cotton gluten meal were found to be highly (100%) contaminated with TA. The OTA was determined in 63.15% (n=72/114) and 29.17% (n=21/72) feed ingredients, and finished feed samples, respectively, with an overall incidence of 50% (n=93/186). Maximum level of OTA contamination (100%) was recorded in corn gluten meal. However, no feed contained OTA above the acceptable level as set by the European Union on OTA contamination in poultry finished feed. On the other hand, a number of samples contained TA above the acceptable limit. Thus, immediate control measures should be taken to ensure safe poultry for human consumption. |
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institution | Directory Open Access Journal |
issn | 2311-7710 2311-7710 |
language | English |
last_indexed | 2024-12-21T05:39:23Z |
publishDate | 2014-12-01 |
publisher | Network for the Veterinarians of Bangladesh |
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series | Journal of Advanced Veterinary and Animal Research |
spelling | doaj.art-fc0568ac7bd4411ebc8bad3185152ea12022-12-21T19:14:19ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102311-77102014-12-011420120710.5455/javar.2014.a38Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environmentGhulam Fareed0Sohail Hassan Khan1Muhammad Ashraf Anjum2Naveed Ahmed3Poultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanPoultry Research Institute, Murree Road, Shamsabad, Rawalpindi, PakistanA total of 186 samples comprising of poultry feed ingredients (n=114) and finished poultry feeds (n=72) were analyzed for the detection of total aflatoxin (TA) and ochratoxin A (OTA). The concentrations of TA and OTA in the samples were determined using direct competitive Enzyme-Linked Immunosorbent Assay (ELISA). Overall incidence of TA was recorded as 80.64% (n=150/186); whereas, in the feed ingredients, it was 86.84% (n=99/114), and in the finished feeds, the incidence of TA was 70.83% (n=51/72). Corn, cotton seed meal, sunflower meal, and cotton gluten meal were found to be highly (100%) contaminated with TA. The OTA was determined in 63.15% (n=72/114) and 29.17% (n=21/72) feed ingredients, and finished feed samples, respectively, with an overall incidence of 50% (n=93/186). Maximum level of OTA contamination (100%) was recorded in corn gluten meal. However, no feed contained OTA above the acceptable level as set by the European Union on OTA contamination in poultry finished feed. On the other hand, a number of samples contained TA above the acceptable limit. Thus, immediate control measures should be taken to ensure safe poultry for human consumption.http://www.scopemed.org/fulltextpdf.php?mno=165557AflatoxinMoistureOchratoxinPoultry feedPoultry feed ingredients |
spellingShingle | Ghulam Fareed Sohail Hassan Khan Muhammad Ashraf Anjum Naveed Ahmed Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment Journal of Advanced Veterinary and Animal Research Aflatoxin Moisture Ochratoxin Poultry feed Poultry feed ingredients |
title | Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment |
title_full | Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment |
title_fullStr | Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment |
title_full_unstemmed | Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment |
title_short | Determination of Aflatoxin and Ochratoxin in poultry feed ingredients and finished feed in humid semi-tropical environment |
title_sort | determination of aflatoxin and ochratoxin in poultry feed ingredients and finished feed in humid semi tropical environment |
topic | Aflatoxin Moisture Ochratoxin Poultry feed Poultry feed ingredients |
url | http://www.scopemed.org/fulltextpdf.php?mno=165557 |
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