LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis
Objective: A lot of lncRNAs are implicated in oral squamous cell carcinoma (OSCC) progression. The study aimed at investigating lncRNA DS cell adhesion molecule antisense RNA 1 (DSCAM-AS1)’s functional role and molecular mechanism in OSCC.Materials and Methods: In this experimental study, a total of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2022-05-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_252920_6c8338fb91f1be731b1962c8d82e7e4c.pdf |
| _version_ | 1818492596985528320 |
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| author | Yanbo Zhang Xuefeng Wang Peng Wang Xingle Zhang Shangzhi Han Feng Huo |
| author_facet | Yanbo Zhang Xuefeng Wang Peng Wang Xingle Zhang Shangzhi Han Feng Huo |
| author_sort | Yanbo Zhang |
| collection | DOAJ |
| description | Objective: A lot of lncRNAs are implicated in oral squamous cell carcinoma (OSCC) progression. The study aimed at investigating lncRNA DS cell adhesion molecule antisense RNA 1 (DSCAM-AS1)’s functional role and molecular mechanism in OSCC.Materials and Methods: In this experimental study, a total of 46 pairs of OSCC samples and para-cancerous tissues were collected during surgery. In OSCC tissues and cell lines, quantitative real time polymerase chain reaction (qRTPCR) was performed for detecting DSCAM-AS1 and microRNA-138-5p (miR-138-5p) expression levels. Western blot was conducted to examine the enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) expression level. Then, DSCAM-AS1 was knocked down with siRNA in OSCC cells and MTT and EdU assays were conducted to evaluate OSCC cell proliferation. Transwell assay was utilized for detecting OSCC cell migration and invasion capacities. Besides, the relationships among DSCAM-AS1, miR-138-5p, and EZH2 were explored through RNA immunoprecipitation, dual-luciferase reporter assay, qRT-PCR, and Western blot.Results: DSCAM-AS1 expression was remarkably increased in OSCC tissues and cell lines, and DSCAM-AS1 knockdown could significantly restrain OSCC cell proliferation, migration, and invasion. MiR-138-5p was identified as a target of DSCAM-AS1, and its inhibitor could reverse the suppressive effects of DSCAM-AS1 knockdown on OSCC progression. EZH2 was verified as a target of miR-138-5p, and EZH2 knockdown could counteract the promotional impact of miR-138-5p inhibitor on OSCC progression. Additionally, DSCAM-AS1, as a ceRNA, could regulate EZH2 expression via miR-138-5p.Conclusion: DSCAM-AS1 can play a tumor-promoting role in OSCC via miR-138-5p/EZH2 axis. |
| first_indexed | 2024-12-10T17:45:14Z |
| format | Article |
| id | doaj.art-fc058ef60cf14a068b657ad4c38864f6 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T17:45:14Z |
| publishDate | 2022-05-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-fc058ef60cf14a068b657ad4c38864f62022-12-22T01:39:16ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-05-0124522222910.22074/cellj.2022.7763252920LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit AxisYanbo Zhang0Xuefeng Wang1Peng Wang2Xingle Zhang3Shangzhi Han4Feng Huo5Department of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaDepartment of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaDepartment of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaDepartment of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaDepartment of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaDepartment of Stomatology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, ChinaObjective: A lot of lncRNAs are implicated in oral squamous cell carcinoma (OSCC) progression. The study aimed at investigating lncRNA DS cell adhesion molecule antisense RNA 1 (DSCAM-AS1)’s functional role and molecular mechanism in OSCC.Materials and Methods: In this experimental study, a total of 46 pairs of OSCC samples and para-cancerous tissues were collected during surgery. In OSCC tissues and cell lines, quantitative real time polymerase chain reaction (qRTPCR) was performed for detecting DSCAM-AS1 and microRNA-138-5p (miR-138-5p) expression levels. Western blot was conducted to examine the enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) expression level. Then, DSCAM-AS1 was knocked down with siRNA in OSCC cells and MTT and EdU assays were conducted to evaluate OSCC cell proliferation. Transwell assay was utilized for detecting OSCC cell migration and invasion capacities. Besides, the relationships among DSCAM-AS1, miR-138-5p, and EZH2 were explored through RNA immunoprecipitation, dual-luciferase reporter assay, qRT-PCR, and Western blot.Results: DSCAM-AS1 expression was remarkably increased in OSCC tissues and cell lines, and DSCAM-AS1 knockdown could significantly restrain OSCC cell proliferation, migration, and invasion. MiR-138-5p was identified as a target of DSCAM-AS1, and its inhibitor could reverse the suppressive effects of DSCAM-AS1 knockdown on OSCC progression. EZH2 was verified as a target of miR-138-5p, and EZH2 knockdown could counteract the promotional impact of miR-138-5p inhibitor on OSCC progression. Additionally, DSCAM-AS1, as a ceRNA, could regulate EZH2 expression via miR-138-5p.Conclusion: DSCAM-AS1 can play a tumor-promoting role in OSCC via miR-138-5p/EZH2 axis.https://www.celljournal.org/article_252920_6c8338fb91f1be731b1962c8d82e7e4c.pdfdscam-as1oral squamous cell carcinomaproliferation |
| spellingShingle | Yanbo Zhang Xuefeng Wang Peng Wang Xingle Zhang Shangzhi Han Feng Huo LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis Cell Journal dscam-as1 oral squamous cell carcinoma proliferation |
| title | LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis |
| title_full | LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis |
| title_fullStr | LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis |
| title_full_unstemmed | LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis |
| title_short | LncRNA DS Cell Adhesion Molecule Antisense RNA 1 Facilitates Oral Squamous Cell Carcinoma Progression through The microRNA-138-5p/ Enhancer of Zeste 2 Polycomb Repressive Complex 2 Subunit Axis |
| title_sort | lncrna ds cell adhesion molecule antisense rna 1 facilitates oral squamous cell carcinoma progression through the microrna 138 5p enhancer of zeste 2 polycomb repressive complex 2 subunit axis |
| topic | dscam-as1 oral squamous cell carcinoma proliferation |
| url | https://www.celljournal.org/article_252920_6c8338fb91f1be731b1962c8d82e7e4c.pdf |
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