Detection of neopterin in the urine of captive and wild platyrrhines
Abstract Background Non-invasive biomarkers can facilitate health assessments in wild primate populations by reducing the need for direct access to animals. Neopterin is a biomarker that is a product of the cell-mediated immune response, with high levels being indicative of poor survival expectation...
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | BMC Zoology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40850-020-00051-9 |
| _version_ | 1819199310929068032 |
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| author | Alexandra J. Sacco Jessica A. Mayhew Mrinalini Watsa Gideon Erkenswick April K. Binder |
| author_facet | Alexandra J. Sacco Jessica A. Mayhew Mrinalini Watsa Gideon Erkenswick April K. Binder |
| author_sort | Alexandra J. Sacco |
| collection | DOAJ |
| description | Abstract Background Non-invasive biomarkers can facilitate health assessments in wild primate populations by reducing the need for direct access to animals. Neopterin is a biomarker that is a product of the cell-mediated immune response, with high levels being indicative of poor survival expectations in some cases. The measurement of urinary neopterin concentration (UNC) has been validated as a method for monitoring cell-mediated immune system activation in multiple catarrhine species, but to date there is no study testing its utility in the urine of platyrrhine species. In this study, we collected urine samples across three platyrrhine families including small captive populations of Leontopithecus rosalia and Pithecia pithecia, and larger wild populations of Leontocebus weddelli, Saguinus imperator, Alouatta seniculus, and Plecturocebus toppini, to evaluate a commercial enzyme-linked immunosorbent assay (ELISA) for the measurement of urinary neopterin in platyrrhines. Results Our results revealed measured UNC fell within the sensitivity range of the assay in all urine samples collected from captive and wild platyrrhine study species via commercial ELISA, and results from several dilutions met expectations. We found significant differences in the mean UNC across all study species. Most notably, we observed higher UNC in the wild population of L. weddelli which is known to have two filarial nematode infections compared to S. imperator, which only have one. Conclusion Our study confirms that neopterin is measurable via commercial ELISA in urine collected from captive and wild individuals of six genera of platyrrhines across three different families. These findings promote the future utility of UNC as a promising biomarker for field primatologists conducting research in Latin America to non-invasively evaluate cell-mediated immune system activation from urine. |
| first_indexed | 2024-12-23T03:14:19Z |
| format | Article |
| id | doaj.art-fc05a90c794147929322daefb73f7992 |
| institution | Directory Open Access Journal |
| issn | 2056-3132 |
| language | English |
| last_indexed | 2024-12-23T03:14:19Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Zoology |
| spelling | doaj.art-fc05a90c794147929322daefb73f79922022-12-21T18:02:12ZengBMCBMC Zoology2056-31322020-02-01511810.1186/s40850-020-00051-9Detection of neopterin in the urine of captive and wild platyrrhinesAlexandra J. Sacco0Jessica A. Mayhew1Mrinalini Watsa2Gideon Erkenswick3April K. Binder4Department of Anthropology, Washington University in St. LouisPrimate Behavior Program, Central Washington UniversityPopulation Sustainability, San Diego Zoo Institute for Conservation ResearchField Projects InternationalPrimate Behavior Program, Central Washington UniversityAbstract Background Non-invasive biomarkers can facilitate health assessments in wild primate populations by reducing the need for direct access to animals. Neopterin is a biomarker that is a product of the cell-mediated immune response, with high levels being indicative of poor survival expectations in some cases. The measurement of urinary neopterin concentration (UNC) has been validated as a method for monitoring cell-mediated immune system activation in multiple catarrhine species, but to date there is no study testing its utility in the urine of platyrrhine species. In this study, we collected urine samples across three platyrrhine families including small captive populations of Leontopithecus rosalia and Pithecia pithecia, and larger wild populations of Leontocebus weddelli, Saguinus imperator, Alouatta seniculus, and Plecturocebus toppini, to evaluate a commercial enzyme-linked immunosorbent assay (ELISA) for the measurement of urinary neopterin in platyrrhines. Results Our results revealed measured UNC fell within the sensitivity range of the assay in all urine samples collected from captive and wild platyrrhine study species via commercial ELISA, and results from several dilutions met expectations. We found significant differences in the mean UNC across all study species. Most notably, we observed higher UNC in the wild population of L. weddelli which is known to have two filarial nematode infections compared to S. imperator, which only have one. Conclusion Our study confirms that neopterin is measurable via commercial ELISA in urine collected from captive and wild individuals of six genera of platyrrhines across three different families. These findings promote the future utility of UNC as a promising biomarker for field primatologists conducting research in Latin America to non-invasively evaluate cell-mediated immune system activation from urine.http://link.springer.com/article/10.1186/s40850-020-00051-9NeopterinHealth monitoringPlatyrrhinesImmune functionBiomarker |
| spellingShingle | Alexandra J. Sacco Jessica A. Mayhew Mrinalini Watsa Gideon Erkenswick April K. Binder Detection of neopterin in the urine of captive and wild platyrrhines BMC Zoology Neopterin Health monitoring Platyrrhines Immune function Biomarker |
| title | Detection of neopterin in the urine of captive and wild platyrrhines |
| title_full | Detection of neopterin in the urine of captive and wild platyrrhines |
| title_fullStr | Detection of neopterin in the urine of captive and wild platyrrhines |
| title_full_unstemmed | Detection of neopterin in the urine of captive and wild platyrrhines |
| title_short | Detection of neopterin in the urine of captive and wild platyrrhines |
| title_sort | detection of neopterin in the urine of captive and wild platyrrhines |
| topic | Neopterin Health monitoring Platyrrhines Immune function Biomarker |
| url | http://link.springer.com/article/10.1186/s40850-020-00051-9 |
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