Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China
Abstract Background Since July in 2015, an emerging infectious disease, Fowl adenovirus (FAdV) species C infection with Hepatitis-Hydropericardium syndrome was prevalent in chicken flocks in China. In our study, one FAdV strain was isolated from commercial broiler chickens and was designated as SDSX...
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BMC
2018-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1733-4 |
| _version_ | 1811325631908020224 |
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| author | Limin Li Jianchang Wang Ping Chen Shan Zhang Jiguo Sun Wanzhe Yuan |
| author_facet | Limin Li Jianchang Wang Ping Chen Shan Zhang Jiguo Sun Wanzhe Yuan |
| author_sort | Limin Li |
| collection | DOAJ |
| description | Abstract Background Since July in 2015, an emerging infectious disease, Fowl adenovirus (FAdV) species C infection with Hepatitis-Hydropericardium syndrome was prevalent in chicken flocks in China. In our study, one FAdV strain was isolated from commercial broiler chickens and was designated as SDSX1.The phylogenetic information, genetic mutations and pathogenicity of SDSX1 were evaluated. Results The phylogenetic analysis indicated that SDSX1 is a strain of serotype 4, FAdV-C. The amino acid analysis of fiber-2 showed that there were more than 20 mutations compared with the non-virulent FAdV-C strains. The pathogenic evaluation of SDSX1 showed that the mortality of one-day-old chickens inoculated SDSX1 was 100%. The typical histopathological changes of SDSX1 were characterized by the presence of basophilic intranuclear inclusion bodies in hepatocytes. The virus copies in different tissues varied from107 to 1011 per 100 mg tissue and liver had the highest virus genome copies. Conclusion In conclusion, the isolate SDSX1, identified as FAdV-4, could cause one-day-old chicks’ typical inclusion body hepatitis (IBH) and hepatitis-hydropericardium syndrome (HHS) with 100% mortality. The virus genome loads were the highest in the liver. Molecular analysis indicated that substitutions in fiber-2 proteins may contribute to the pathogenicity of SDSX1. |
| first_indexed | 2024-04-13T14:36:35Z |
| format | Article |
| id | doaj.art-fc087d143d5f4384b5850dc5db41aac3 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-13T14:36:35Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-fc087d143d5f4384b5850dc5db41aac32022-12-22T02:43:01ZengBMCBMC Veterinary Research1746-61482018-12-011411810.1186/s12917-018-1733-4Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in ChinaLimin Li0Jianchang Wang1Ping Chen2Shan Zhang3Jiguo Sun4Wanzhe Yuan5College of Veterinary Medicine, Hebei Agricultural UniversityInspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine BureauCollege of Veterinary Medicine, Hebei Agricultural UniversityCollege of Veterinary Medicine, Hebei Agricultural UniversityCollege of Veterinary Medicine, Hebei Agricultural UniversityCollege of Veterinary Medicine, Hebei Agricultural UniversityAbstract Background Since July in 2015, an emerging infectious disease, Fowl adenovirus (FAdV) species C infection with Hepatitis-Hydropericardium syndrome was prevalent in chicken flocks in China. In our study, one FAdV strain was isolated from commercial broiler chickens and was designated as SDSX1.The phylogenetic information, genetic mutations and pathogenicity of SDSX1 were evaluated. Results The phylogenetic analysis indicated that SDSX1 is a strain of serotype 4, FAdV-C. The amino acid analysis of fiber-2 showed that there were more than 20 mutations compared with the non-virulent FAdV-C strains. The pathogenic evaluation of SDSX1 showed that the mortality of one-day-old chickens inoculated SDSX1 was 100%. The typical histopathological changes of SDSX1 were characterized by the presence of basophilic intranuclear inclusion bodies in hepatocytes. The virus copies in different tissues varied from107 to 1011 per 100 mg tissue and liver had the highest virus genome copies. Conclusion In conclusion, the isolate SDSX1, identified as FAdV-4, could cause one-day-old chicks’ typical inclusion body hepatitis (IBH) and hepatitis-hydropericardium syndrome (HHS) with 100% mortality. The virus genome loads were the highest in the liver. Molecular analysis indicated that substitutions in fiber-2 proteins may contribute to the pathogenicity of SDSX1.http://link.springer.com/article/10.1186/s12917-018-1733-4Fowl adenovirus 4Hydropericardium syndromeInclusion body hepatitisVirulentPathogenicityPhylogenetic analysis |
| spellingShingle | Limin Li Jianchang Wang Ping Chen Shan Zhang Jiguo Sun Wanzhe Yuan Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China BMC Veterinary Research Fowl adenovirus 4 Hydropericardium syndrome Inclusion body hepatitis Virulent Pathogenicity Phylogenetic analysis |
| title | Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China |
| title_full | Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China |
| title_fullStr | Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China |
| title_full_unstemmed | Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China |
| title_short | Pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with IBH and HPS in China |
| title_sort | pathogenicity and molecular characterization of a fowl adenovirus 4 isolated from chicken associated with ibh and hps in china |
| topic | Fowl adenovirus 4 Hydropericardium syndrome Inclusion body hepatitis Virulent Pathogenicity Phylogenetic analysis |
| url | http://link.springer.com/article/10.1186/s12917-018-1733-4 |
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