Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro
This study investigated the effect of dielectric barrier discharge (DBD) on sea cucumber polysaccharide (SP-2) and evaluated its anti-inflammatory properties. The SP-2 was depolymerized by applying an input voltage of 60~90 V for 3~9 min. The features of the products were examined using high-perform...
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MDPI AG
2023-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/22/4079 |
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| author | Shiwen Cheng Han Cai Meng Yi Liang Dong Jingfeng Yang |
| author_facet | Shiwen Cheng Han Cai Meng Yi Liang Dong Jingfeng Yang |
| author_sort | Shiwen Cheng |
| collection | DOAJ |
| description | This study investigated the effect of dielectric barrier discharge (DBD) on sea cucumber polysaccharide (SP-2) and evaluated its anti-inflammatory properties. The SP-2 was depolymerized by applying an input voltage of 60~90 V for 3~9 min. The features of the products were examined using high-performance gel permeation chromatography, HPLC-PAD-MS, and the Fourier transform infrared (FTIR) spectrum. The anti-inflammatory properties of the product were investigated by measuring nitric oxide (NO) release, ROS accumulation, and cell migration using RAW264.7 cells (LPS-induced or not-induced). The results showed SP-2 depolymerized into homogeneous and controllable-size oligosaccharide products. The depolymerized ratio can reach 80%. The results of the measurement of reducing sugars indicate that SP-2 was cleaved from within the sugar chain. The SP-2 was deduced to have a monosaccharide sequence of GlcN-Man-Man-Man-Man-Man based on the digested fragment information. The depolymerization product restrained the release of NO and the accumulation of ROS. By testing the RAW264.7 cell scratch assay, it was found that it enhances the migration of immune cells. DBD degradation of SP-2 leads to homogeneous and controllable-size oligosaccharide products, and this technique can be used for polysaccharide structure analysis. The depolymerized product of SP-2 has an anti-inflammatory capability in vitro. |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T16:50:24Z |
| publishDate | 2023-11-01 |
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| spelling | doaj.art-fc09ed96f3e74c608e75f4b6949f1ac82023-11-24T14:41:58ZengMDPI AGFoods2304-81582023-11-011222407910.3390/foods12224079Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In VitroShiwen Cheng0Han Cai1Meng Yi2Liang Dong3Jingfeng Yang4School of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaThis study investigated the effect of dielectric barrier discharge (DBD) on sea cucumber polysaccharide (SP-2) and evaluated its anti-inflammatory properties. The SP-2 was depolymerized by applying an input voltage of 60~90 V for 3~9 min. The features of the products were examined using high-performance gel permeation chromatography, HPLC-PAD-MS, and the Fourier transform infrared (FTIR) spectrum. The anti-inflammatory properties of the product were investigated by measuring nitric oxide (NO) release, ROS accumulation, and cell migration using RAW264.7 cells (LPS-induced or not-induced). The results showed SP-2 depolymerized into homogeneous and controllable-size oligosaccharide products. The depolymerized ratio can reach 80%. The results of the measurement of reducing sugars indicate that SP-2 was cleaved from within the sugar chain. The SP-2 was deduced to have a monosaccharide sequence of GlcN-Man-Man-Man-Man-Man based on the digested fragment information. The depolymerization product restrained the release of NO and the accumulation of ROS. By testing the RAW264.7 cell scratch assay, it was found that it enhances the migration of immune cells. DBD degradation of SP-2 leads to homogeneous and controllable-size oligosaccharide products, and this technique can be used for polysaccharide structure analysis. The depolymerized product of SP-2 has an anti-inflammatory capability in vitro.https://www.mdpi.com/2304-8158/12/22/4079dielectric barrier dischargepolysaccharidedegradationRAW264.7 cellmigration |
| spellingShingle | Shiwen Cheng Han Cai Meng Yi Liang Dong Jingfeng Yang Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro Foods dielectric barrier discharge polysaccharide degradation RAW264.7 cell migration |
| title | Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro |
| title_full | Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro |
| title_fullStr | Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro |
| title_full_unstemmed | Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro |
| title_short | Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro |
| title_sort | degradation product of sea cucumber polysaccharide by dielectric barrier discharge enhanced the migration of macrophage in vitro |
| topic | dielectric barrier discharge polysaccharide degradation RAW264.7 cell migration |
| url | https://www.mdpi.com/2304-8158/12/22/4079 |
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