Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses
Abstract Melatonin plays important roles in multiple stress responses; however, the downstream signaling pathway and molecular mechanism remain unclear. This study aimed to elucidate the transcriptional regulation of melatonin-induced salt stress tolerance in Phaseolus vulgaris L. and identify the k...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04705-x |
| _version_ | 1797276650081091584 |
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| author | Xiaoxu Yang Dajun Liu Chang Liu Mengdi Li Zhishan Yan Yu Zhang Guojun Feng |
| author_facet | Xiaoxu Yang Dajun Liu Chang Liu Mengdi Li Zhishan Yan Yu Zhang Guojun Feng |
| author_sort | Xiaoxu Yang |
| collection | DOAJ |
| description | Abstract Melatonin plays important roles in multiple stress responses; however, the downstream signaling pathway and molecular mechanism remain unclear. This study aimed to elucidate the transcriptional regulation of melatonin-induced salt stress tolerance in Phaseolus vulgaris L. and identify the key downstream transcription factors of melatonin through transcriptomic and metabolomic analyses. The melatonin-induced transcriptional network of hormones, transcription factors, and functional genes was established under both control and stress conditions. Among these, eight candidate transcription factors were identified via gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, one gene related to transmembrane transport of salts (Phvul.004G177300). These genes may play a role in maintaining the cell structure and excreting sodium ions outside the cell or transporting them to the vacuoles for storage. Melatonin regulates the Phvul.009G210332 gene and metabolites C05642 (N-acetyl-N-2-formyl-5-methoxycanurine), C05643 (6-hydroxymelatonin), C05660 (5-methoxyindoleacetic acid) involved in tryptophan metabolism. The metabolites C05642 and C05643 were identified as decomposition products of tryptophan, indicating that exogenous melatonin entered the P. vulgaris tissue and was metabolized. Melatonin promotes the synthesis and metabolism of tryptophan, which is crucial to plant metabolism, growth, maintenance, and repair. |
| first_indexed | 2024-03-07T15:32:14Z |
| format | Article |
| id | doaj.art-fc0a8d547a694a4c993aaf4deda58ad0 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-07T15:32:14Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-fc0a8d547a694a4c993aaf4deda58ad02024-03-05T16:24:46ZengBMCBMC Plant Biology1471-22292024-01-0124111410.1186/s12870-023-04705-xPossible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analysesXiaoxu Yang0Dajun Liu1Chang Liu2Mengdi Li3Zhishan Yan4Yu Zhang5Guojun Feng6Horticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityHorticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang UniversityAbstract Melatonin plays important roles in multiple stress responses; however, the downstream signaling pathway and molecular mechanism remain unclear. This study aimed to elucidate the transcriptional regulation of melatonin-induced salt stress tolerance in Phaseolus vulgaris L. and identify the key downstream transcription factors of melatonin through transcriptomic and metabolomic analyses. The melatonin-induced transcriptional network of hormones, transcription factors, and functional genes was established under both control and stress conditions. Among these, eight candidate transcription factors were identified via gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, one gene related to transmembrane transport of salts (Phvul.004G177300). These genes may play a role in maintaining the cell structure and excreting sodium ions outside the cell or transporting them to the vacuoles for storage. Melatonin regulates the Phvul.009G210332 gene and metabolites C05642 (N-acetyl-N-2-formyl-5-methoxycanurine), C05643 (6-hydroxymelatonin), C05660 (5-methoxyindoleacetic acid) involved in tryptophan metabolism. The metabolites C05642 and C05643 were identified as decomposition products of tryptophan, indicating that exogenous melatonin entered the P. vulgaris tissue and was metabolized. Melatonin promotes the synthesis and metabolism of tryptophan, which is crucial to plant metabolism, growth, maintenance, and repair.https://doi.org/10.1186/s12870-023-04705-xCommon beanSalt stressMelatoninTranscriptomeMetabolome |
| spellingShingle | Xiaoxu Yang Dajun Liu Chang Liu Mengdi Li Zhishan Yan Yu Zhang Guojun Feng Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses BMC Plant Biology Common bean Salt stress Melatonin Transcriptome Metabolome |
| title | Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses |
| title_full | Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses |
| title_fullStr | Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses |
| title_full_unstemmed | Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses |
| title_short | Possible melatonin-induced salt stress tolerance pathway in Phaseolus vulgaris L. using transcriptomic and metabolomic analyses |
| title_sort | possible melatonin induced salt stress tolerance pathway in phaseolus vulgaris l using transcriptomic and metabolomic analyses |
| topic | Common bean Salt stress Melatonin Transcriptome Metabolome |
| url | https://doi.org/10.1186/s12870-023-04705-x |
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