Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions
Various activities of different industries are found to be the main reason for water pollution with heavy metals. Use of microorganisms that are tolerant even of a high concentration of metal ions could be a valuable tool for remediation of contaminated water resources. In the present study, microor...
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-04-01
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| Series: | Water Science and Technology |
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| Online Access: | http://wst.iwaponline.com/content/85/8/2358 |
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| author | Vinay Kumar Chintalapudi Ramya Krishna S. L. Kanamarlapudi Useni Reddy Mallu Sudhamani Muddada |
| author_facet | Vinay Kumar Chintalapudi Ramya Krishna S. L. Kanamarlapudi Useni Reddy Mallu Sudhamani Muddada |
| author_sort | Vinay Kumar Chintalapudi |
| collection | DOAJ |
| description | Various activities of different industries are found to be the main reason for water pollution with heavy metals. Use of microorganisms that are tolerant even of a high concentration of metal ions could be a valuable tool for remediation of contaminated water resources. In the present study, microorganisms that showed high resistance to lead ions were isolated and evaluated for biosorption efficiency for removal of lead ions from waste water. Biochemical identification and 16S rRNA gene sequence analysis indicated that the isolated strain was Brevibacillus. The conditions of pH, biomass concentration, temperature, time, agitation and Initial concentration of metal for biosorption of Pb (II) were optimized. Based on induction coupled plasma optical emission spectroscopy (ICP-OES) analysis, the biosorption efficiency of Brevibacillus at optimized conditions of initial metal concentration of 150 μg/mL, 1 g/L of biomass dose, pH 6.0, 40 °C, for 12 h at 80 rpm was 78.58% and the biosorption capacity (qe) is 128.58 mg/g of the biosorbent. Of the three isotherm models investigated, the Freundlich isotherm model was identified as a good fit with high correlation coefficient, while kinetic data followed the pseudo first order model as best fit. Surface characterization by scanning electron microscopy (SEM) analysis revealed morphological changes with a bulged rod-shape cell having metal depositions and rough texture. The presence of lead within the cell was detected by transmission emission microscopy (TEM). The key functional groups that participate in biosorption were analyzed by Fourier transform infrared (FTIR) spectroscopy and were found to be carboxyl, hydroxyl, amino and phosphate groups. From the real-time study, it proves that the biomass of Brevibacillus can be used as a promising biosorbent for removal of metals including lead from waste water. HIGHLIGHTS
The biosorption potential of Brevibacillus biomass for metal ions was evaluated for the first time in the literature.;
Surface characterization of biomass before and after biosorption was performed by SEM-EDX, FTIR and TEM.;
Isotherm and kinetic data was evaluated from the optimized experimental results.;
The Brevibacillus biomass was evaluated for its potential to remove Pb (II) from contaminated water sources in real-time studies.; |
| first_indexed | 2024-12-12T18:57:15Z |
| format | Article |
| id | doaj.art-fc0b526bc83c4250af77ce3160632987 |
| institution | Directory Open Access Journal |
| issn | 0273-1223 1996-9732 |
| language | English |
| last_indexed | 2024-12-12T18:57:15Z |
| publishDate | 2022-04-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Science and Technology |
| spelling | doaj.art-fc0b526bc83c4250af77ce31606329872022-12-22T00:15:14ZengIWA PublishingWater Science and Technology0273-12231996-97322022-04-018582358237410.2166/wst.2022.110110Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ionsVinay Kumar Chintalapudi0Ramya Krishna S. L. Kanamarlapudi1Useni Reddy Mallu2Sudhamani Muddada3 R V Labs, 2-14-117-55, 3rd lane extension, Syamala nagar, Guntur, Andhra Pradesh, India Department of Biotechnology, Koneru Lakshmaiah Education Foundation (KLEF), Deemed to be University, Greenfields, Vaddeswaram, Guntur-522502, Andhra Pradesh, India R V Labs, 2-14-117-55, 3rd lane extension, Syamala nagar, Guntur, Andhra Pradesh, India Department of Biotechnology, Koneru Lakshmaiah Education Foundation (KLEF), Deemed to be University, Greenfields, Vaddeswaram, Guntur-522502, Andhra Pradesh, India Various activities of different industries are found to be the main reason for water pollution with heavy metals. Use of microorganisms that are tolerant even of a high concentration of metal ions could be a valuable tool for remediation of contaminated water resources. In the present study, microorganisms that showed high resistance to lead ions were isolated and evaluated for biosorption efficiency for removal of lead ions from waste water. Biochemical identification and 16S rRNA gene sequence analysis indicated that the isolated strain was Brevibacillus. The conditions of pH, biomass concentration, temperature, time, agitation and Initial concentration of metal for biosorption of Pb (II) were optimized. Based on induction coupled plasma optical emission spectroscopy (ICP-OES) analysis, the biosorption efficiency of Brevibacillus at optimized conditions of initial metal concentration of 150 μg/mL, 1 g/L of biomass dose, pH 6.0, 40 °C, for 12 h at 80 rpm was 78.58% and the biosorption capacity (qe) is 128.58 mg/g of the biosorbent. Of the three isotherm models investigated, the Freundlich isotherm model was identified as a good fit with high correlation coefficient, while kinetic data followed the pseudo first order model as best fit. Surface characterization by scanning electron microscopy (SEM) analysis revealed morphological changes with a bulged rod-shape cell having metal depositions and rough texture. The presence of lead within the cell was detected by transmission emission microscopy (TEM). The key functional groups that participate in biosorption were analyzed by Fourier transform infrared (FTIR) spectroscopy and were found to be carboxyl, hydroxyl, amino and phosphate groups. From the real-time study, it proves that the biomass of Brevibacillus can be used as a promising biosorbent for removal of metals including lead from waste water. HIGHLIGHTS The biosorption potential of Brevibacillus biomass for metal ions was evaluated for the first time in the literature.; Surface characterization of biomass before and after biosorption was performed by SEM-EDX, FTIR and TEM.; Isotherm and kinetic data was evaluated from the optimized experimental results.; The Brevibacillus biomass was evaluated for its potential to remove Pb (II) from contaminated water sources in real-time studies.;http://wst.iwaponline.com/content/85/8/2358biosorptionbrevibacillusheavy metalsisothermskineticslead |
| spellingShingle | Vinay Kumar Chintalapudi Ramya Krishna S. L. Kanamarlapudi Useni Reddy Mallu Sudhamani Muddada Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions Water Science and Technology biosorption brevibacillus heavy metals isotherms kinetics lead |
| title | Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions |
| title_full | Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions |
| title_fullStr | Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions |
| title_full_unstemmed | Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions |
| title_short | Characterization of biosorption potential of Brevibacillus biomass isolated from contaminated water resources for removal of Pb (II) ions |
| title_sort | characterization of biosorption potential of brevibacillus biomass isolated from contaminated water resources for removal of pb ii ions |
| topic | biosorption brevibacillus heavy metals isotherms kinetics lead |
| url | http://wst.iwaponline.com/content/85/8/2358 |
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