Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers
Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.941134/full |
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| author | Adriana Mércia Guaratini Ibelli Adriana Mércia Guaratini Ibelli Jane de Oliveira Peixoto Jane de Oliveira Peixoto Ricardo Zanella João José de Simoni Gouveia Maurício Egídio Cantão Luiz Lehmann Coutinho Jorge Augusto Petroli Marchesi Mariane Spudeit dal Pizzol Débora Ester Petry Marcelino Mônica Corrêa Ledur Mônica Corrêa Ledur |
| author_facet | Adriana Mércia Guaratini Ibelli Adriana Mércia Guaratini Ibelli Jane de Oliveira Peixoto Jane de Oliveira Peixoto Ricardo Zanella João José de Simoni Gouveia Maurício Egídio Cantão Luiz Lehmann Coutinho Jorge Augusto Petroli Marchesi Mariane Spudeit dal Pizzol Débora Ester Petry Marcelino Mônica Corrêa Ledur Mônica Corrêa Ledur |
| author_sort | Adriana Mércia Guaratini Ibelli |
| collection | DOAJ |
| description | Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers. |
| first_indexed | 2024-04-13T20:00:49Z |
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| id | doaj.art-fc0c66cc66a44ff09ac8e3c67e6d3451 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T20:00:49Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-fc0c66cc66a44ff09ac8e3c67e6d34512022-12-22T02:32:12ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-08-011310.3389/fphys.2022.941134941134Downregulation of growth plate genes involved with the onset of femoral head separation in young broilersAdriana Mércia Guaratini Ibelli0Adriana Mércia Guaratini Ibelli1Jane de Oliveira Peixoto2Jane de Oliveira Peixoto3Ricardo Zanella4João José de Simoni Gouveia5Maurício Egídio Cantão6Luiz Lehmann Coutinho7Jorge Augusto Petroli Marchesi8Mariane Spudeit dal Pizzol9Débora Ester Petry Marcelino10Mônica Corrêa Ledur11Mônica Corrêa Ledur12Embrapa Suínos e Aves, Concórdia, BrazilPrograma de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, Guarapuava, BrazilEmbrapa Suínos e Aves, Concórdia, BrazilPrograma de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, Guarapuava, BrazilUniversidade de Passo Fundo, Passo Fundo, BrazilUniversidade Federal do Vale do São Francisco, UNIVASF, Petrolina, BrazilEmbrapa Suínos e Aves, Concórdia, BrazilLaboratório de Biotecnologia Animal, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de SP, Piracicaba, BrazilDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de SP, Ribeirão Preto, BrazilPrograma de Pós-Graduação Em Zootecnia, Universidade do Estado de SC, UDESC-Oeste, Chapecó, BrazilFaculdade de Concórdia FACC, Concórdia, BrazilEmbrapa Suínos e Aves, Concórdia, BrazilPrograma de Pós-Graduação Em Zootecnia, Universidade do Estado de SC, UDESC-Oeste, Chapecó, BrazilFemoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR ≤0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers.https://www.frontiersin.org/articles/10.3389/fphys.2022.941134/fullchickensgene expressionFBN2FHSfemoral head necrosisRNA-seq |
| spellingShingle | Adriana Mércia Guaratini Ibelli Adriana Mércia Guaratini Ibelli Jane de Oliveira Peixoto Jane de Oliveira Peixoto Ricardo Zanella João José de Simoni Gouveia Maurício Egídio Cantão Luiz Lehmann Coutinho Jorge Augusto Petroli Marchesi Mariane Spudeit dal Pizzol Débora Ester Petry Marcelino Mônica Corrêa Ledur Mônica Corrêa Ledur Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers Frontiers in Physiology chickens gene expression FBN2 FHS femoral head necrosis RNA-seq |
| title | Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| title_full | Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| title_fullStr | Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| title_full_unstemmed | Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| title_short | Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| title_sort | downregulation of growth plate genes involved with the onset of femoral head separation in young broilers |
| topic | chickens gene expression FBN2 FHS femoral head necrosis RNA-seq |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.941134/full |
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