Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid
Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange. Resistance plasmid confer resistance to many antibiotics and...
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| Format: | Article |
| Language: | fas |
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Hamadan University of Medical Sciences
1999-09-01
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| Series: | پزشکی بالینی ابن سینا |
| Subjects: | |
| Online Access: | http://sjh.umsha.ac.ir/article-1-985-en.html |
| _version_ | 1818268808649900032 |
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| author | Mohammad Javad Kajbaf Mohammad Ali Mashhadizadeh Heidar Reza Mohtadi |
| author_facet | Mohammad Javad Kajbaf Mohammad Ali Mashhadizadeh Heidar Reza Mohtadi |
| author_sort | Mohammad Javad Kajbaf |
| collection | DOAJ |
| description | Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange. Resistance plasmid confer resistance to many antibiotics and in this group, penicillinase plasmid is responsible for resistance to penicillins and cephalosprins. In this study, 300 E.coli strains which had been isolated from urinary tract infections were tested by a rapid acidometric method for the presence of penicillinase, of which 95% showed to be positive. 40 penicillinase producing strain were later subjected to artificial conjunction and 25% of them transferred this plasmid to recipient bacteria. The results indicate high potential for this kind of antibiotic resistance and it’s transfer. |
| first_indexed | 2024-12-12T20:44:23Z |
| format | Article |
| id | doaj.art-fc0ce9fced374ed5a33980bb3e3b68d4 |
| institution | Directory Open Access Journal |
| issn | 2588-722X 2588-7238 |
| language | fas |
| last_indexed | 2024-12-12T20:44:23Z |
| publishDate | 1999-09-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | پزشکی بالینی ابن سینا |
| spelling | doaj.art-fc0ce9fced374ed5a33980bb3e3b68d42022-12-22T00:12:38ZfasHamadan University of Medical Sciencesپزشکی بالینی ابن سینا2588-722X2588-72381999-09-016200Artificial Conjugation in Escherichia coli and Transfer of Penicillinase PlasmidMohammad Javad Kajbaf0Mohammad Ali Mashhadizadeh1Heidar Reza Mohtadi2 Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange. Resistance plasmid confer resistance to many antibiotics and in this group, penicillinase plasmid is responsible for resistance to penicillins and cephalosprins. In this study, 300 E.coli strains which had been isolated from urinary tract infections were tested by a rapid acidometric method for the presence of penicillinase, of which 95% showed to be positive. 40 penicillinase producing strain were later subjected to artificial conjunction and 25% of them transferred this plasmid to recipient bacteria. The results indicate high potential for this kind of antibiotic resistance and it’s transfer.http://sjh.umsha.ac.ir/article-1-985-en.htmlconjugationgenetic / escherichia coli / penicillinase |
| spellingShingle | Mohammad Javad Kajbaf Mohammad Ali Mashhadizadeh Heidar Reza Mohtadi Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid پزشکی بالینی ابن سینا conjugation genetic / escherichia coli / penicillinase |
| title | Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid |
| title_full | Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid |
| title_fullStr | Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid |
| title_full_unstemmed | Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid |
| title_short | Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid |
| title_sort | artificial conjugation in escherichia coli and transfer of penicillinase plasmid |
| topic | conjugation genetic / escherichia coli / penicillinase |
| url | http://sjh.umsha.ac.ir/article-1-985-en.html |
| work_keys_str_mv | AT mohammadjavadkajbaf artificialconjugationinescherichiacoliandtransferofpenicillinaseplasmid AT mohammadalimashhadizadeh artificialconjugationinescherichiacoliandtransferofpenicillinaseplasmid AT heidarrezamohtadi artificialconjugationinescherichiacoliandtransferofpenicillinaseplasmid |