Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma
Background: Skin cancer is the most frequent cancer worldwide and is divided into non-melanoma skin cancer, including basal cell carcinoma, as well as squamous cell carcinoma (SCC) and malignant melanoma (MM). Methods: This study evaluates the effects of cold atmospheric pressure plasma (CAP) on SCC...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/10/2483 |
| _version_ | 1797533520249225216 |
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| author | Marcel Kordt Isabell Trautmann Christin Schlie Tobias Lindner Jan Stenzel Anna Schildt Lars Boeckmann Sander Bekeschus Jens Kurth Bernd J. Krause Brigitte Vollmar Eberhard Grambow |
| author_facet | Marcel Kordt Isabell Trautmann Christin Schlie Tobias Lindner Jan Stenzel Anna Schildt Lars Boeckmann Sander Bekeschus Jens Kurth Bernd J. Krause Brigitte Vollmar Eberhard Grambow |
| author_sort | Marcel Kordt |
| collection | DOAJ |
| description | Background: Skin cancer is the most frequent cancer worldwide and is divided into non-melanoma skin cancer, including basal cell carcinoma, as well as squamous cell carcinoma (SCC) and malignant melanoma (MM). Methods: This study evaluates the effects of cold atmospheric pressure plasma (CAP) on SCC and MM in vivo, employing a comprehensive approach using multimodal imaging techniques. Longitudinal MR and PET/CT imaging were performed to determine the anatomic and metabolic tumour volume over three-weeks in vivo. Additionally, the formation of reactive species after CAP treatment was assessed by non-invasive chemiluminescence imaging of L-012. Histological analysis and immunohistochemical staining for Ki-67, ApopTag<sup>®</sup>, F4/80, CAE, and CD31, as well as protein expression of PCNA, caspase-3 and cleaved-caspase-3, were performed to study proliferation, apoptosis, inflammation, and angiogenesis in CAP-treated tumours. Results: As the main result, multimodal in vivo imaging revealed a substantial reduction in tumour growth and an increase in reactive species after CAP treatment, in comparison to untreated tumours. In contrast, neither the markers for apoptosis, nor the metabolic activity of both tumour entities was affected by CAP. Conclusions: These findings propose CAP as a potential adjuvant therapy option to established standard therapies of skin cancer. |
| first_indexed | 2024-03-10T11:15:43Z |
| format | Article |
| id | doaj.art-fc0d4fc3c58b472aa66e304e8c778d8b |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T11:15:43Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-fc0d4fc3c58b472aa66e304e8c778d8b2023-11-21T20:28:46ZengMDPI AGCancers2072-66942021-05-011310248310.3390/cancers13102483Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure PlasmaMarcel Kordt0Isabell Trautmann1Christin Schlie2Tobias Lindner3Jan Stenzel4Anna Schildt5Lars Boeckmann6Sander Bekeschus7Jens Kurth8Bernd J. Krause9Brigitte Vollmar10Eberhard Grambow11Rudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, GermanyRudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, GermanyRudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, GermanyCore Facility Multimodal Small Animal Imaging, Rostock University Medical Center, 18057 Rostock, GermanyCore Facility Multimodal Small Animal Imaging, Rostock University Medical Center, 18057 Rostock, GermanyCore Facility Multimodal Small Animal Imaging, Rostock University Medical Center, 18057 Rostock, GermanyClinic and Policlinic for Dermatology and Venereology, Rostock University Medical Center, 18057 Rostock, GermanyCenter for innovation competence (ZIK) <i>plasmatis</i>, Leibniz Institute for Plasma Science and Technology (INP), 17489 Greifswald, GermanyDepartment of Nuclear Medicine, Rostock University Medical Center, 18055 Rostock, GermanyCore Facility Multimodal Small Animal Imaging, Rostock University Medical Center, 18057 Rostock, GermanyRudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, GermanyRudolf-Zenker-Institute of Experimental Surgery, Rostock University Medical Center, 18057 Rostock, GermanyBackground: Skin cancer is the most frequent cancer worldwide and is divided into non-melanoma skin cancer, including basal cell carcinoma, as well as squamous cell carcinoma (SCC) and malignant melanoma (MM). Methods: This study evaluates the effects of cold atmospheric pressure plasma (CAP) on SCC and MM in vivo, employing a comprehensive approach using multimodal imaging techniques. Longitudinal MR and PET/CT imaging were performed to determine the anatomic and metabolic tumour volume over three-weeks in vivo. Additionally, the formation of reactive species after CAP treatment was assessed by non-invasive chemiluminescence imaging of L-012. Histological analysis and immunohistochemical staining for Ki-67, ApopTag<sup>®</sup>, F4/80, CAE, and CD31, as well as protein expression of PCNA, caspase-3 and cleaved-caspase-3, were performed to study proliferation, apoptosis, inflammation, and angiogenesis in CAP-treated tumours. Results: As the main result, multimodal in vivo imaging revealed a substantial reduction in tumour growth and an increase in reactive species after CAP treatment, in comparison to untreated tumours. In contrast, neither the markers for apoptosis, nor the metabolic activity of both tumour entities was affected by CAP. Conclusions: These findings propose CAP as a potential adjuvant therapy option to established standard therapies of skin cancer.https://www.mdpi.com/2072-6694/13/10/2483kINPen™malignant melanomaplasma medicinereactive oxygen and nitrogen speciesskin cancersquamous cell carcinoma |
| spellingShingle | Marcel Kordt Isabell Trautmann Christin Schlie Tobias Lindner Jan Stenzel Anna Schildt Lars Boeckmann Sander Bekeschus Jens Kurth Bernd J. Krause Brigitte Vollmar Eberhard Grambow Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma Cancers kINPen™ malignant melanoma plasma medicine reactive oxygen and nitrogen species skin cancer squamous cell carcinoma |
| title | Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma |
| title_full | Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma |
| title_fullStr | Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma |
| title_full_unstemmed | Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma |
| title_short | Multimodal Imaging Techniques to Evaluate the Anticancer Effect of Cold Atmospheric Pressure Plasma |
| title_sort | multimodal imaging techniques to evaluate the anticancer effect of cold atmospheric pressure plasma |
| topic | kINPen™ malignant melanoma plasma medicine reactive oxygen and nitrogen species skin cancer squamous cell carcinoma |
| url | https://www.mdpi.com/2072-6694/13/10/2483 |
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