Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin
Skin rejuvenation procedures such as microneedling and laser resurfacing have gained global popularity in medical cosmetology, leading to acute skin wounds with persistent pain, erythema, and edema. A variety of dressings have been explored to repair these postoperative skin injuries; however, their...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Cosmetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-9284/10/1/31 |
| _version_ | 1827758007049519104 |
|---|---|
| author | Caihong Fu Shuangni Shi Nannan Wei Yirui Fan Hong Gu Peng Liu Jianxi Xiao |
| author_facet | Caihong Fu Shuangni Shi Nannan Wei Yirui Fan Hong Gu Peng Liu Jianxi Xiao |
| author_sort | Caihong Fu |
| collection | DOAJ |
| description | Skin rejuvenation procedures such as microneedling and laser resurfacing have gained global popularity in medical cosmetology, leading to acute skin wounds with persistent pain, erythema, and edema. A variety of dressings have been explored to repair these postoperative skin injuries; however, their inadequate biocompatibility and bioactivity may raise concerns about undesirable efficacy and complications. Herein, we developed biocompatible and nonirritating triple-helical recombinant collagen (THRC) dressings for accelerated healing of microneedle-injured and photodamaged acute skin wounds. Circular dichroism (CD) measurements of THRC from various batches exhibited triple-helical structure characteristics of collagen. Cell experiments using L929 fibroblasts revealed that THRC dressings possess superior biocompatibility and bioactivity, significantly elevating the proliferation and adhesion of fibroblasts. In vivo, skin irritation tests of New Zealand rabbits demonstrated that the THRC dressings are gentle, safe, and non-irritating. Histological analysis of the animal model studies in photodamaged skin wounds using H&E and Masson’s trichrome staining revealed that 4 days of treatment with the THRC dressings effectively healed the damaged dermis by accelerating re-epithelialization and enhancing collagen deposition. In vivo studies of microneedle-injured rat defects showed that THRC dressings of varying concentrations exhibit the same rapid epithelialization rates at 48 h as commercial bovine collagen dressings. The highly biocompatible and bioactive recombinant collagen dressings may provide an advanced treatment of acute skin wounds, indicating attractive applications in postoperative care of facial rejuvenation. |
| first_indexed | 2024-03-11T08:59:29Z |
| format | Article |
| id | doaj.art-fc113faf07824b4ea0e423b2f68ab7ee |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-11T08:59:29Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-fc113faf07824b4ea0e423b2f68ab7ee2023-11-16T19:53:52ZengMDPI AGCosmetics2079-92842023-02-011013110.3390/cosmetics10010031Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged SkinCaihong Fu0Shuangni Shi1Nannan Wei2Yirui Fan3Hong Gu4Peng Liu5Jianxi Xiao6State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, ChinaState Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, ChinaState Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, ChinaState Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, ChinaSchool of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, ChinaGansu Engineering Research Center of Medical Collagen, Lanzhou 730000, ChinaState Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, ChinaSkin rejuvenation procedures such as microneedling and laser resurfacing have gained global popularity in medical cosmetology, leading to acute skin wounds with persistent pain, erythema, and edema. A variety of dressings have been explored to repair these postoperative skin injuries; however, their inadequate biocompatibility and bioactivity may raise concerns about undesirable efficacy and complications. Herein, we developed biocompatible and nonirritating triple-helical recombinant collagen (THRC) dressings for accelerated healing of microneedle-injured and photodamaged acute skin wounds. Circular dichroism (CD) measurements of THRC from various batches exhibited triple-helical structure characteristics of collagen. Cell experiments using L929 fibroblasts revealed that THRC dressings possess superior biocompatibility and bioactivity, significantly elevating the proliferation and adhesion of fibroblasts. In vivo, skin irritation tests of New Zealand rabbits demonstrated that the THRC dressings are gentle, safe, and non-irritating. Histological analysis of the animal model studies in photodamaged skin wounds using H&E and Masson’s trichrome staining revealed that 4 days of treatment with the THRC dressings effectively healed the damaged dermis by accelerating re-epithelialization and enhancing collagen deposition. In vivo studies of microneedle-injured rat defects showed that THRC dressings of varying concentrations exhibit the same rapid epithelialization rates at 48 h as commercial bovine collagen dressings. The highly biocompatible and bioactive recombinant collagen dressings may provide an advanced treatment of acute skin wounds, indicating attractive applications in postoperative care of facial rejuvenation.https://www.mdpi.com/2079-9284/10/1/31recombinant collagen dressingskin rejuvenationwound healingmicroneedlingphotodamaged skin |
| spellingShingle | Caihong Fu Shuangni Shi Nannan Wei Yirui Fan Hong Gu Peng Liu Jianxi Xiao Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin Cosmetics recombinant collagen dressing skin rejuvenation wound healing microneedling photodamaged skin |
| title | Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin |
| title_full | Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin |
| title_fullStr | Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin |
| title_full_unstemmed | Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin |
| title_short | Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin |
| title_sort | biocompatible triple helical recombinant collagen dressings for accelerated wound healing in microneedle injured and photodamaged skin |
| topic | recombinant collagen dressing skin rejuvenation wound healing microneedling photodamaged skin |
| url | https://www.mdpi.com/2079-9284/10/1/31 |
| work_keys_str_mv | AT caihongfu biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT shuangnishi biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT nannanwei biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT yiruifan biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT honggu biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT pengliu biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin AT jianxixiao biocompatibletriplehelicalrecombinantcollagendressingsforacceleratedwoundhealinginmicroneedleinjuredandphotodamagedskin |