The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency
Pectin, cellulose, and hemicellulose constitute the primary cell wall in eudicots and function in multiple developmental processes in plants. Root hairs are outgrowths of specialized epidermal cells that absorb water and nutrients from the soil. Cell wall architecture influences root hair developmen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.862171/full |
| _version_ | 1818150175637504000 |
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| author | Qing Zhang Aiwen Deng Min Xiang Qiuyan Lan Xiaokun Li Shuai Yuan Xin Gou Shuang Hao Juan Du Chaowen Xiao |
| author_facet | Qing Zhang Aiwen Deng Min Xiang Qiuyan Lan Xiaokun Li Shuai Yuan Xin Gou Shuang Hao Juan Du Chaowen Xiao |
| author_sort | Qing Zhang |
| collection | DOAJ |
| description | Pectin, cellulose, and hemicellulose constitute the primary cell wall in eudicots and function in multiple developmental processes in plants. Root hairs are outgrowths of specialized epidermal cells that absorb water and nutrients from the soil. Cell wall architecture influences root hair development, but how cell wall remodeling might enable enhanced root hair formation in response to phosphate (P) deficiency remains relatively unclear. Here, we found that POLYGALACTURONASE INVOLVED IN EXPANSION 2 (PGX2) functions in conditional root hair development. Under low P conditions, a PGX2 activation tagged line (PGX2AT) displays bubble-like root hairs and abnormal callose deposition and superoxide accumulation in roots. We found that the polar localization and trafficking of PIN2 are altered in PGX2AT roots in response to P deficiency. We also found that actin filaments were less compact but more stable in PGX2AT root hair cells and that actin filament skewness in PGX2AT root hairs was recovered by treatment with 1-N-naphthylphthalamic acid (NPA), an auxin transport inhibitor. These results demonstrate that activation tagging of PGX2 affects cell wall remodeling, auxin signaling, and actin microfilament orientation, which may cooperatively regulate root hair development in response to P starvation. |
| first_indexed | 2024-12-11T13:18:45Z |
| format | Article |
| id | doaj.art-fc114b84a3ca4002bcd0eceb4735c334 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T13:18:45Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fc114b84a3ca4002bcd0eceb4735c3342022-12-22T01:05:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.862171862171The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate DeficiencyQing ZhangAiwen DengMin XiangQiuyan LanXiaokun LiShuai YuanXin GouShuang HaoJuan DuChaowen XiaoPectin, cellulose, and hemicellulose constitute the primary cell wall in eudicots and function in multiple developmental processes in plants. Root hairs are outgrowths of specialized epidermal cells that absorb water and nutrients from the soil. Cell wall architecture influences root hair development, but how cell wall remodeling might enable enhanced root hair formation in response to phosphate (P) deficiency remains relatively unclear. Here, we found that POLYGALACTURONASE INVOLVED IN EXPANSION 2 (PGX2) functions in conditional root hair development. Under low P conditions, a PGX2 activation tagged line (PGX2AT) displays bubble-like root hairs and abnormal callose deposition and superoxide accumulation in roots. We found that the polar localization and trafficking of PIN2 are altered in PGX2AT roots in response to P deficiency. We also found that actin filaments were less compact but more stable in PGX2AT root hair cells and that actin filament skewness in PGX2AT root hairs was recovered by treatment with 1-N-naphthylphthalamic acid (NPA), an auxin transport inhibitor. These results demonstrate that activation tagging of PGX2 affects cell wall remodeling, auxin signaling, and actin microfilament orientation, which may cooperatively regulate root hair development in response to P starvation.https://www.frontiersin.org/articles/10.3389/fpls.2022.862171/fullcell wallpectinpolygalacturonasephosphate deficiencyroot hair developmentArabidopsis thaliana |
| spellingShingle | Qing Zhang Aiwen Deng Min Xiang Qiuyan Lan Xiaokun Li Shuai Yuan Xin Gou Shuang Hao Juan Du Chaowen Xiao The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency Frontiers in Plant Science cell wall pectin polygalacturonase phosphate deficiency root hair development Arabidopsis thaliana |
| title | The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency |
| title_full | The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency |
| title_fullStr | The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency |
| title_full_unstemmed | The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency |
| title_short | The Root Hair Development of Pectin Polygalacturonase PGX2 Activation Tagging Line in Response to Phosphate Deficiency |
| title_sort | root hair development of pectin polygalacturonase pgx2 activation tagging line in response to phosphate deficiency |
| topic | cell wall pectin polygalacturonase phosphate deficiency root hair development Arabidopsis thaliana |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.862171/full |
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