Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections.
Intestinal capillariasis is a parasitic zoonosis caused by the tiny nematode parasite Capillaria philippinensis. It is a major health problem that may lead to death if not diagnosed and treated appropriately. The difficulties in the diagnosis of C. philippinensis highlight the importance of developi...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0234746 |
| _version_ | 1819125497155551232 |
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| author | Mervat M Khalifa Salma M Abdel-Rahman Hanaa Y Bakir Ragaa A Othman Mohamed A El-Mokhtar |
| author_facet | Mervat M Khalifa Salma M Abdel-Rahman Hanaa Y Bakir Ragaa A Othman Mohamed A El-Mokhtar |
| author_sort | Mervat M Khalifa |
| collection | DOAJ |
| description | Intestinal capillariasis is a parasitic zoonosis caused by the tiny nematode parasite Capillaria philippinensis. It is a major health problem that may lead to death if not diagnosed and treated appropriately. The difficulties in the diagnosis of C. philippinensis highlight the importance of developing accurate, sensitive, and specific methods for early diagnosis. This study aimed to detect the presence of C. philippinensis infection among 42 clinically suspected patients with certain criteria that are highly suggestive of capillariasis and to compare the diagnostic yield of microscopy, copro-ELISA, and PCR for the detection of copro-DNA. Sociodemographic characteristics and clinical data were also described for the infected group. Out of 42 patients, 10 were microscopically positive, 40 samples were positive by copro-ELISA, nested PCR detected 35 positive cases, with total detection rates of 23.8%, 95.2%, and 83.3% using direct microscopic examination, copro-ELISA, and PCR, respectively. The majority of positive cases were females, middle-aged people, and people from rural areas. The real number of cases infected with C. philippinensis may far exceed those estimated using microscopy. The diagnosis by copro-ELISA for the detection of C. philippinensis coproantigen and by nested PCR to identify parasite DNA revealed a higher number of positive cases. Using ELISA for the detection of coproantigen is a sensitive test that identifies the infection, yet it is not specific. Copro-DNA offers a satisfactory sensitive and specific method for the detection of infection in clinically suspected patients. The most susceptible individuals to C. philippinensis infection are females, middle-aged people, and people of low social standards. Intestinal capillariasis needs to be considered in patients who present with symptoms of chronic diarrhea and hypoalbuminemia because if these cases are left undiagnosed and untreated, they may suffer from lethal complications. |
| first_indexed | 2024-12-22T07:41:04Z |
| format | Article |
| id | doaj.art-fc13266f3da64a468ff4737ef6f69d65 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:41:04Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fc13266f3da64a468ff4737ef6f69d652022-12-21T18:33:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023474610.1371/journal.pone.0234746Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections.Mervat M KhalifaSalma M Abdel-RahmanHanaa Y BakirRagaa A OthmanMohamed A El-MokhtarIntestinal capillariasis is a parasitic zoonosis caused by the tiny nematode parasite Capillaria philippinensis. It is a major health problem that may lead to death if not diagnosed and treated appropriately. The difficulties in the diagnosis of C. philippinensis highlight the importance of developing accurate, sensitive, and specific methods for early diagnosis. This study aimed to detect the presence of C. philippinensis infection among 42 clinically suspected patients with certain criteria that are highly suggestive of capillariasis and to compare the diagnostic yield of microscopy, copro-ELISA, and PCR for the detection of copro-DNA. Sociodemographic characteristics and clinical data were also described for the infected group. Out of 42 patients, 10 were microscopically positive, 40 samples were positive by copro-ELISA, nested PCR detected 35 positive cases, with total detection rates of 23.8%, 95.2%, and 83.3% using direct microscopic examination, copro-ELISA, and PCR, respectively. The majority of positive cases were females, middle-aged people, and people from rural areas. The real number of cases infected with C. philippinensis may far exceed those estimated using microscopy. The diagnosis by copro-ELISA for the detection of C. philippinensis coproantigen and by nested PCR to identify parasite DNA revealed a higher number of positive cases. Using ELISA for the detection of coproantigen is a sensitive test that identifies the infection, yet it is not specific. Copro-DNA offers a satisfactory sensitive and specific method for the detection of infection in clinically suspected patients. The most susceptible individuals to C. philippinensis infection are females, middle-aged people, and people of low social standards. Intestinal capillariasis needs to be considered in patients who present with symptoms of chronic diarrhea and hypoalbuminemia because if these cases are left undiagnosed and untreated, they may suffer from lethal complications.https://doi.org/10.1371/journal.pone.0234746 |
| spellingShingle | Mervat M Khalifa Salma M Abdel-Rahman Hanaa Y Bakir Ragaa A Othman Mohamed A El-Mokhtar Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. PLoS ONE |
| title | Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. |
| title_full | Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. |
| title_fullStr | Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. |
| title_full_unstemmed | Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. |
| title_short | Comparison of the diagnostic performance of microscopic examination, Copro-ELISA, and Copro-PCR in the diagnosis of Capillaria philippinensis infections. |
| title_sort | comparison of the diagnostic performance of microscopic examination copro elisa and copro pcr in the diagnosis of capillaria philippinensis infections |
| url | https://doi.org/10.1371/journal.pone.0234746 |
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