Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice
Functional characterization of stress-responsive genes through the analysis of transgenic plants is a standard approach to comprehend their role in climate resilience and subsequently exploit them for sustainable crop improvement. In this study, we investigated the function of LOC_Os04g59420, a gene...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.947312/full |
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| author | Karikalan Jayaraman Karikalan Jayaraman Amitha Mithra Sevanthi Kalappan Venkat Raman Gitanjali Jiwani Amolkumar U. Solanke Pranab Kumar Mandal Trilochan Mohapatra |
| author_facet | Karikalan Jayaraman Karikalan Jayaraman Amitha Mithra Sevanthi Kalappan Venkat Raman Gitanjali Jiwani Amolkumar U. Solanke Pranab Kumar Mandal Trilochan Mohapatra |
| author_sort | Karikalan Jayaraman |
| collection | DOAJ |
| description | Functional characterization of stress-responsive genes through the analysis of transgenic plants is a standard approach to comprehend their role in climate resilience and subsequently exploit them for sustainable crop improvement. In this study, we investigated the function of LOC_Os04g59420, a gene of DUF740 family (OsSRDP-Oryza sativaStress Responsive DUF740 Protein) from rice, which showed upregulation in response to abiotic stress in the available global expression data, but is yet to be functionally characterized. Transgenic plants of the rice OsSRDP gene, driven by a stress-inducible promoter AtRd29A, were developed in the background of cv. Pusa Sugandh 2 (PS2) and their transgene integration and copy number were confirmed by molecular analysis. The three independent homozygous transgenic plants (AtRd29A::OsSRDP rice transformants) showed better resilience to drought, salinity, and cold stresses, but not heat stress, as compared to the non-transformed PS2, which corresponded with their respective relative transcript abundance for OsSRDP. Transgenic plants maintained higher RWC, photosynthetic pigments, and proline accumulation under drought and salinity stresses. Furthermore, they exhibited less accumulation of reactive oxygen species (ROS) than PS2 under drought stress, as seen from the transcript abundance studies of the ROS genes. Under cold stress, OsSRDP transgenic lines illustrated minimal cell membrane injury compared to PS2. Additionally, the transgenic plants showed resistance to a virulent strain of rice blast fungus, Magnaporthe oryzae (M. oryzae). The promoter analysis of the gene in N22 and PS2 revealed the presence of multiple abiotic and biotic stress-specific motif elements supporting our observation on multiple stress tolerance. Based on bioinformatics studies, we identified four potential candidate interaction partners for LOC_Os04g59420, of which two genes (LOC_Os05g09640 and LOC_Os06g50370) showed co-expression under biotic and drought stress along with OsSRDP. Altogether, our findings established that stress-inducible expression of OsSRDP can significantly enhance tolerance to multiple abiotic stresses and a biotic stress. |
| first_indexed | 2024-04-10T21:36:33Z |
| format | Article |
| id | doaj.art-fc1570be7a984e148b2a2b3d5d94896e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T21:36:33Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-fc1570be7a984e148b2a2b3d5d94896e2023-01-19T11:55:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.947312947312Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in riceKarikalan Jayaraman0Karikalan Jayaraman1Amitha Mithra Sevanthi2Kalappan Venkat Raman3Gitanjali Jiwani4Amolkumar U. Solanke5Pranab Kumar Mandal6Trilochan Mohapatra7Indian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaDepartment of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu, IndiaIndian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaIndian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaIndian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaIndian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaIndian Council of Agricultural Research (ICAR) - National Institute for Plant Biotechnology, New Delhi, IndiaIndian Council of Agricultural Research (ICAR), Krishi Bhawan, New Delhi, IndiaFunctional characterization of stress-responsive genes through the analysis of transgenic plants is a standard approach to comprehend their role in climate resilience and subsequently exploit them for sustainable crop improvement. In this study, we investigated the function of LOC_Os04g59420, a gene of DUF740 family (OsSRDP-Oryza sativaStress Responsive DUF740 Protein) from rice, which showed upregulation in response to abiotic stress in the available global expression data, but is yet to be functionally characterized. Transgenic plants of the rice OsSRDP gene, driven by a stress-inducible promoter AtRd29A, were developed in the background of cv. Pusa Sugandh 2 (PS2) and their transgene integration and copy number were confirmed by molecular analysis. The three independent homozygous transgenic plants (AtRd29A::OsSRDP rice transformants) showed better resilience to drought, salinity, and cold stresses, but not heat stress, as compared to the non-transformed PS2, which corresponded with their respective relative transcript abundance for OsSRDP. Transgenic plants maintained higher RWC, photosynthetic pigments, and proline accumulation under drought and salinity stresses. Furthermore, they exhibited less accumulation of reactive oxygen species (ROS) than PS2 under drought stress, as seen from the transcript abundance studies of the ROS genes. Under cold stress, OsSRDP transgenic lines illustrated minimal cell membrane injury compared to PS2. Additionally, the transgenic plants showed resistance to a virulent strain of rice blast fungus, Magnaporthe oryzae (M. oryzae). The promoter analysis of the gene in N22 and PS2 revealed the presence of multiple abiotic and biotic stress-specific motif elements supporting our observation on multiple stress tolerance. Based on bioinformatics studies, we identified four potential candidate interaction partners for LOC_Os04g59420, of which two genes (LOC_Os05g09640 and LOC_Os06g50370) showed co-expression under biotic and drought stress along with OsSRDP. Altogether, our findings established that stress-inducible expression of OsSRDP can significantly enhance tolerance to multiple abiotic stresses and a biotic stress.https://www.frontiersin.org/articles/10.3389/fpls.2022.947312/fullriceLOC_Os04g59420DUF740 familyabiotic stressROSrice blast disease |
| spellingShingle | Karikalan Jayaraman Karikalan Jayaraman Amitha Mithra Sevanthi Kalappan Venkat Raman Gitanjali Jiwani Amolkumar U. Solanke Pranab Kumar Mandal Trilochan Mohapatra Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice Frontiers in Plant Science rice LOC_Os04g59420 DUF740 family abiotic stress ROS rice blast disease |
| title | Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice |
| title_full | Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice |
| title_fullStr | Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice |
| title_full_unstemmed | Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice |
| title_short | Overexpression of a DUF740 family gene (LOC_Os04g59420) imparts enhanced climate resilience through multiple stress tolerance in rice |
| title_sort | overexpression of a duf740 family gene loc os04g59420 imparts enhanced climate resilience through multiple stress tolerance in rice |
| topic | rice LOC_Os04g59420 DUF740 family abiotic stress ROS rice blast disease |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.947312/full |
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