An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell
AbstractElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the el...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2239274 |
| _version_ | 1827765367493099520 |
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| author | Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petrů Seyed Saeid Rahimian Koloor |
| author_facet | Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petrů Seyed Saeid Rahimian Koloor |
| author_sort | Muhammad Asraf Mansor |
| collection | DOAJ |
| description | AbstractElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microchannel for single-cell detection and electrical properties extraction. The dual microneedles utilized a commercially available tungsten needle coated with parylene. When a single cell flows through the parallel-facing electrode configuration of the dual microneedle, the electrical impedance at multiple frequencies is measured. The impedance measurement demonstrated the differential of normal red blood cells (RBCs) with three different sizes of microbeads at low and high frequencies, 100 kHz and 2 MHz, respectively. An electrical equivalent circuit model (ECM) was used to determine the unique membrane capacitance of individual cells. The proposed technique demonstrated that the specific membrane capacitance of an RBC is 9.42 mF/m−2, with the regression coefficients, [Formula: see text] at 0.9895. As a result, this device may potentially be used in developing countries for low-cost single-cell screening and detection. |
| first_indexed | 2024-03-11T11:18:02Z |
| format | Article |
| id | doaj.art-fc16a884d7c9479b8307a70754d4b5bb |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:18:02Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-fc16a884d7c9479b8307a70754d4b5bb2023-11-10T17:19:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151137138310.1080/21691401.2023.2239274An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cellMuhammad Asraf Mansor0Mohd Ridzuan Ahmad1Michal Petrů2Seyed Saeid Rahimian Koloor3Department of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Skudai, MalaysiaDepartment of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Skudai, MalaysiaFaculty of Mechanical Engineering, Technical University of Liberec, Liberec, Czech RepublicInstitute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Liberec, Czech RepublicAbstractElectrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microchannel for single-cell detection and electrical properties extraction. The dual microneedles utilized a commercially available tungsten needle coated with parylene. When a single cell flows through the parallel-facing electrode configuration of the dual microneedle, the electrical impedance at multiple frequencies is measured. The impedance measurement demonstrated the differential of normal red blood cells (RBCs) with three different sizes of microbeads at low and high frequencies, 100 kHz and 2 MHz, respectively. An electrical equivalent circuit model (ECM) was used to determine the unique membrane capacitance of individual cells. The proposed technique demonstrated that the specific membrane capacitance of an RBC is 9.42 mF/m−2, with the regression coefficients, [Formula: see text] at 0.9895. As a result, this device may potentially be used in developing countries for low-cost single-cell screening and detection.https://www.tandfonline.com/doi/10.1080/21691401.2023.2239274Single-cell analysisimpedance analysismicrofluidicimpedance flow cytometry |
| spellingShingle | Muhammad Asraf Mansor Mohd Ridzuan Ahmad Michal Petrů Seyed Saeid Rahimian Koloor An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell Artificial Cells, Nanomedicine, and Biotechnology Single-cell analysis impedance analysis microfluidic impedance flow cytometry |
| title | An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| title_full | An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| title_fullStr | An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| title_full_unstemmed | An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| title_short | An impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| title_sort | impedance flow cytometry with integrated dual microneedle for electrical properties characterization of single cell |
| topic | Single-cell analysis impedance analysis microfluidic impedance flow cytometry |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2239274 |
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