Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats
Background: In the pathogenesis of central nervous system disorders (e.g., neurodegenerative), an important role is attributed to an unhealthy lifestyle affecting brain energy metabolism. Physical activity in the prevention and treatment of lifestyle-related diseases is getting increasing attention....
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MDPI AG
2021-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/12/4242 |
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| author | Marta Maria Nowacka-Chmielewska Daniela Liśkiewicz Konstancja Grabowska Arkadiusz Liśkiewicz Łukasz Marczak Anna Wojakowska Natalia Pondel Mateusz Grabowski Jarosław Jerzy Barski Andrzej Małecki |
| author_facet | Marta Maria Nowacka-Chmielewska Daniela Liśkiewicz Konstancja Grabowska Arkadiusz Liśkiewicz Łukasz Marczak Anna Wojakowska Natalia Pondel Mateusz Grabowski Jarosław Jerzy Barski Andrzej Małecki |
| author_sort | Marta Maria Nowacka-Chmielewska |
| collection | DOAJ |
| description | Background: In the pathogenesis of central nervous system disorders (e.g., neurodegenerative), an important role is attributed to an unhealthy lifestyle affecting brain energy metabolism. Physical activity in the prevention and treatment of lifestyle-related diseases is getting increasing attention. Methods: We performed a series of assessments in adult female Long Evans rats subjected to 6 weeks of Western diet feeding and wheel-running training. A control group of lean rats was fed with a standard diet. In all experimental groups, we measured physiological parameters (animal weights, body composition, serum metabolic parameters). We assessed the impact of simultaneous exposure to a Western diet and wheel-running on the cerebrocortical protein expression (global proteomic profiling), and in the second part of the experiment, we measured the cortical levels of protein related to brain metabolism (Western blot). Results: Western diet led to an obese phenotype and induced changes in the serum metabolic parameters. Wheel-running did not reduce animal weights or fat mass but significantly decreased serum glucose level. The global proteome analysis revealed that the altered proteins were functionally annotated as they were involved mostly in metabolic pathways. Western blot analysis showed the downregulation of the mitochondrial protein—Acyl-CoA dehydrogenase family member 9, hexokinase 1 (HK1)—enzyme involved in principal glucose metabolism pathways and monocarboxylate transporter 2 (MCT2). Wheel-running reversed this decline in the cortical levels of HK1 and MCT2. Conclusion: The cerebrocortical proteome is affected by a combination of physical activity and Western diet in female rats. An analysis of the cortical proteins involved in brain energy metabolism provides a valuable basis for the deeper investigation of changes in the brain structure and function induced by simultaneous exposure to a Western diet and physical activity. |
| first_indexed | 2024-03-10T03:24:57Z |
| format | Article |
| id | doaj.art-fc1722125d9c4a00a60897de0e30cdc0 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:24:57Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-fc1722125d9c4a00a60897de0e30cdc02023-11-23T09:54:58ZengMDPI AGNutrients2072-66432021-11-011312424210.3390/nu13124242Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female RatsMarta Maria Nowacka-Chmielewska0Daniela Liśkiewicz1Konstancja Grabowska2Arkadiusz Liśkiewicz3Łukasz Marczak4Anna Wojakowska5Natalia Pondel6Mateusz Grabowski7Jarosław Jerzy Barski8Andrzej Małecki9Laboratory of Molecular Biology, Institute of Physiotherapy and Health Sciences, Academy of Physical Education, 40-065 Katowice, PolandLaboratory of Molecular Biology, Institute of Physiotherapy and Health Sciences, Academy of Physical Education, 40-065 Katowice, PolandDepartment for Experimental Medicine, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Physiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandInstitute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznań, PolandInstitute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznań, PolandLaboratory of Molecular Biology, Institute of Physiotherapy and Health Sciences, Academy of Physical Education, 40-065 Katowice, PolandDepartment for Experimental Medicine, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandDepartment for Experimental Medicine, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandLaboratory of Molecular Biology, Institute of Physiotherapy and Health Sciences, Academy of Physical Education, 40-065 Katowice, PolandBackground: In the pathogenesis of central nervous system disorders (e.g., neurodegenerative), an important role is attributed to an unhealthy lifestyle affecting brain energy metabolism. Physical activity in the prevention and treatment of lifestyle-related diseases is getting increasing attention. Methods: We performed a series of assessments in adult female Long Evans rats subjected to 6 weeks of Western diet feeding and wheel-running training. A control group of lean rats was fed with a standard diet. In all experimental groups, we measured physiological parameters (animal weights, body composition, serum metabolic parameters). We assessed the impact of simultaneous exposure to a Western diet and wheel-running on the cerebrocortical protein expression (global proteomic profiling), and in the second part of the experiment, we measured the cortical levels of protein related to brain metabolism (Western blot). Results: Western diet led to an obese phenotype and induced changes in the serum metabolic parameters. Wheel-running did not reduce animal weights or fat mass but significantly decreased serum glucose level. The global proteome analysis revealed that the altered proteins were functionally annotated as they were involved mostly in metabolic pathways. Western blot analysis showed the downregulation of the mitochondrial protein—Acyl-CoA dehydrogenase family member 9, hexokinase 1 (HK1)—enzyme involved in principal glucose metabolism pathways and monocarboxylate transporter 2 (MCT2). Wheel-running reversed this decline in the cortical levels of HK1 and MCT2. Conclusion: The cerebrocortical proteome is affected by a combination of physical activity and Western diet in female rats. An analysis of the cortical proteins involved in brain energy metabolism provides a valuable basis for the deeper investigation of changes in the brain structure and function induced by simultaneous exposure to a Western diet and physical activity.https://www.mdpi.com/2072-6643/13/12/4242western dietwheel-running trainingfemale ratsglobal brain proteomebrain energy metabolism |
| spellingShingle | Marta Maria Nowacka-Chmielewska Daniela Liśkiewicz Konstancja Grabowska Arkadiusz Liśkiewicz Łukasz Marczak Anna Wojakowska Natalia Pondel Mateusz Grabowski Jarosław Jerzy Barski Andrzej Małecki Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats Nutrients western diet wheel-running training female rats global brain proteome brain energy metabolism |
| title | Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats |
| title_full | Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats |
| title_fullStr | Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats |
| title_full_unstemmed | Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats |
| title_short | Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats |
| title_sort | effects of simultaneous exposure to a western diet and wheel running training on brain energy metabolism in female rats |
| topic | western diet wheel-running training female rats global brain proteome brain energy metabolism |
| url | https://www.mdpi.com/2072-6643/13/12/4242 |
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