Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
Bacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resis...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/10/5079 |
| _version_ | 1797534563107340288 |
|---|---|
| author | Constance Porrini Cyprien Guérin Seav-Ly Tran Rozenn Dervyn Pierre Nicolas Nalini Ramarao |
| author_facet | Constance Porrini Cyprien Guérin Seav-Ly Tran Rozenn Dervyn Pierre Nicolas Nalini Ramarao |
| author_sort | Constance Porrini |
| collection | DOAJ |
| description | Bacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resistance by Gram-positive bacteria are poorly described. In the opportunistic foodborne pathogen <i>Bacillus cereus</i>, genome sequence analyses did not identify homologs to known NO reductases and transcriptional regulators, such as NsrR, which orchestrate the response to NO of other pathogenic or non-pathogenic bacteria. Using a transcriptomic approach, we investigated the adaptation of <i>B. cereus</i> to NO stress. A cluster of 6 genes was identified to be strongly up-regulated in the early phase of the response. This cluster contains an iron-sulfur cluster repair enzyme, a nitrite reductase and three enzymes involved in siroheme biosynthesis. The expression pattern and close genetic localization suggest a functional link between these genes, which may play a pivotal role in the resistance of <i>B. cereus</i> to NO stress during infection. |
| first_indexed | 2024-03-10T11:32:17Z |
| format | Article |
| id | doaj.art-fc18cca0439b4f59bd4d54420c732272 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:32:17Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fc18cca0439b4f59bd4d54420c7322722023-11-21T19:12:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012210507910.3390/ijms22105079Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide StressConstance Porrini0Cyprien Guérin1Seav-Ly Tran2Rozenn Dervyn3Pierre Nicolas4Nalini Ramarao5Micalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMaIAGE, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMaIAGE, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceBacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resistance by Gram-positive bacteria are poorly described. In the opportunistic foodborne pathogen <i>Bacillus cereus</i>, genome sequence analyses did not identify homologs to known NO reductases and transcriptional regulators, such as NsrR, which orchestrate the response to NO of other pathogenic or non-pathogenic bacteria. Using a transcriptomic approach, we investigated the adaptation of <i>B. cereus</i> to NO stress. A cluster of 6 genes was identified to be strongly up-regulated in the early phase of the response. This cluster contains an iron-sulfur cluster repair enzyme, a nitrite reductase and three enzymes involved in siroheme biosynthesis. The expression pattern and close genetic localization suggest a functional link between these genes, which may play a pivotal role in the resistance of <i>B. cereus</i> to NO stress during infection.https://www.mdpi.com/1422-0067/22/10/5079<i>Bacillus cereus</i>nitric oxideiron cluster repairtranscriptomic |
| spellingShingle | Constance Porrini Cyprien Guérin Seav-Ly Tran Rozenn Dervyn Pierre Nicolas Nalini Ramarao Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress International Journal of Molecular Sciences <i>Bacillus cereus</i> nitric oxide iron cluster repair transcriptomic |
| title | Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress |
| title_full | Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress |
| title_fullStr | Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress |
| title_full_unstemmed | Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress |
| title_short | Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress |
| title_sort | implication of a key region of six i bacillus cereus i genes involved in siroheme synthesis nitrite reductase production and iron cluster repair in the bacterial response to nitric oxide stress |
| topic | <i>Bacillus cereus</i> nitric oxide iron cluster repair transcriptomic |
| url | https://www.mdpi.com/1422-0067/22/10/5079 |
| work_keys_str_mv | AT constanceporrini implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress AT cyprienguerin implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress AT seavlytran implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress AT rozenndervyn implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress AT pierrenicolas implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress AT naliniramarao implicationofakeyregionofsixibacilluscereusigenesinvolvedinsirohemesynthesisnitritereductaseproductionandironclusterrepairinthebacterialresponsetonitricoxidestress |