PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate
The peel color of pomegranates is an important exterior quality that determines market value. Anthocyanins are biosynthesized in the cytosol and then transported to the vacuole for storage. However, the molecular mechanism that determines the color variation between red and white pomegranates remain...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6366 |
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| author | Zenghui Wang Xuemei Yang Chuanzeng Wang Lijuan Feng Yanlei Yin Jialin Li |
| author_facet | Zenghui Wang Xuemei Yang Chuanzeng Wang Lijuan Feng Yanlei Yin Jialin Li |
| author_sort | Zenghui Wang |
| collection | DOAJ |
| description | The peel color of pomegranates is an important exterior quality that determines market value. Anthocyanins are biosynthesized in the cytosol and then transported to the vacuole for storage. However, the molecular mechanism that determines the color variation between red and white pomegranates remains unclear. In this study, we identified an R2R3-MYB protein (PgMYB1) that interacts with the <i>PgGSTF6</i> promoter and regulates its transcriptional expression, thus promoting the accumulation of anthocyanins in pomegranate. The expression of <i>PgMYB1</i> and <i>PgGSTF6</i> was positively correlated with the anthocyanin content in red and white pomegranates. Further investigation showed that the knockdown of <i>PgMYB1</i> in red pomegranate ‘Taishanhong’ (TSH), by the virus-induced gene-silencing system, inhibited anthocyanin accumulation. Together, our results indicate that PgMYB1 controls the transport of anthocyanin via <i>PgGSTF6</i> and thus promotes anthocyanin accumulation in red pomegranates. Our results have a certain reference value for further clarifying the regulation of anthocyanin synthesis and transport in pomegranate fruits. |
| first_indexed | 2024-03-11T05:36:25Z |
| format | Article |
| id | doaj.art-fc1a18b2f752442c839a417d71ac0683 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:36:25Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fc1a18b2f752442c839a417d71ac06832023-11-17T16:50:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247636610.3390/ijms24076366PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in PomegranateZenghui Wang0Xuemei Yang1Chuanzeng Wang2Lijuan Feng3Yanlei Yin4Jialin Li5Shandong Institute of Pomology, Tai’an 271000, ChinaShandong Institute of Pomology, Tai’an 271000, ChinaShandong Institute of Pomology, Tai’an 271000, ChinaShandong Institute of Pomology, Tai’an 271000, ChinaShandong Institute of Pomology, Tai’an 271000, ChinaSchool of Biological Science and Technology, University of Jinan, Jinan 250022, ChinaThe peel color of pomegranates is an important exterior quality that determines market value. Anthocyanins are biosynthesized in the cytosol and then transported to the vacuole for storage. However, the molecular mechanism that determines the color variation between red and white pomegranates remains unclear. In this study, we identified an R2R3-MYB protein (PgMYB1) that interacts with the <i>PgGSTF6</i> promoter and regulates its transcriptional expression, thus promoting the accumulation of anthocyanins in pomegranate. The expression of <i>PgMYB1</i> and <i>PgGSTF6</i> was positively correlated with the anthocyanin content in red and white pomegranates. Further investigation showed that the knockdown of <i>PgMYB1</i> in red pomegranate ‘Taishanhong’ (TSH), by the virus-induced gene-silencing system, inhibited anthocyanin accumulation. Together, our results indicate that PgMYB1 controls the transport of anthocyanin via <i>PgGSTF6</i> and thus promotes anthocyanin accumulation in red pomegranates. Our results have a certain reference value for further clarifying the regulation of anthocyanin synthesis and transport in pomegranate fruits.https://www.mdpi.com/1422-0067/24/7/6366anthocyaninPgMYB1<i>PgGSTF6</i>pomegranatetranscriptional expression |
| spellingShingle | Zenghui Wang Xuemei Yang Chuanzeng Wang Lijuan Feng Yanlei Yin Jialin Li PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate International Journal of Molecular Sciences anthocyanin PgMYB1 <i>PgGSTF6</i> pomegranate transcriptional expression |
| title | PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate |
| title_full | PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate |
| title_fullStr | PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate |
| title_full_unstemmed | PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate |
| title_short | PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating <i>PgGSTF6</i> in Pomegranate |
| title_sort | pgmyb1 positively regulates anthocyanin accumulation by activating i pggstf6 i in pomegranate |
| topic | anthocyanin PgMYB1 <i>PgGSTF6</i> pomegranate transcriptional expression |
| url | https://www.mdpi.com/1422-0067/24/7/6366 |
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