Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine
To introduce testing of Grapevine leafroll-associated virus-2 (GLRaV-2) and Grapevine virus B (GVB) in sanitary selection of grapevine, commercially available antibodies were evaluated and conditions for routine ELISA testing were optimized. Extraction procedure with Granex 91 - special machine, wh...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2008-05-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15169 |
| _version_ | 1797390852791730176 |
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| author | Irma TOMAŽIČ Irena MAVRIČ PLEŠKO Nataša PETROVIČ Maja RAVNIKAR Zora KOROŠEC-KORUZA |
| author_facet | Irma TOMAŽIČ Irena MAVRIČ PLEŠKO Nataša PETROVIČ Maja RAVNIKAR Zora KOROŠEC-KORUZA |
| author_sort | Irma TOMAŽIČ |
| collection | DOAJ |
| description |
To introduce testing of Grapevine leafroll-associated virus-2 (GLRaV-2) and Grapevine virus B (GVB) in sanitary selection of grapevine, commercially available antibodies were evaluated and conditions for routine ELISA testing were optimized. Extraction procedure with Granex 91 - special machine, which is used in routine testing in Slovenia, was compared with grinding samples in mortar. Three different extraction buffers were applied in order to overcome the inconvenience of using more than one extraction procedure when testing grapevine material for several viruses in a routine large-scale testing scheme. Results were verified with Western blot and immuno-electron microscopy. The best results were obtained using extraction buffer with unknown composition (pH 9.0) from BIOREBA kit for GLRaV-2. Other extraction buffers gave less positive samples and they are not convenient for routine testing where extraction with Granex is done. Both viruses, GLRaV-2 and GVB were found in Slovenia, but they couldn't be correlated with rougose wood disease that appears on indigenous cultivar Refošk grafted on 'SO4' from collection vineyard in Komen.
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| first_indexed | 2024-03-08T23:23:20Z |
| format | Article |
| id | doaj.art-fc1c2873a18a4de888d6de8dd8801c02 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T23:23:20Z |
| publishDate | 2008-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-fc1c2873a18a4de888d6de8dd8801c022023-12-14T22:15:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412008-05-01911Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine Irma TOMAŽIČ0Irena MAVRIČ PLEŠKO1Nataša PETROVIČ2Maja RAVNIKAR3Zora KOROŠEC-KORUZA4University of Primorska – Science and Research Centre of Koper, SI- 6000 Koper, Garibaldijeva 1Agricultural Institute of Slovenia, SI-1000, Ljubljana, Hacquetova 17National Institute of Biology, SI-1000, Večna pot 111National Institute of Biology, SI-1000, Večna pot 111University of Ljubljana, Biotechnical Faculty, Department of Agronomy, Jamnikarjeva 101, p.p. 2995, SI-1111, Ljubljana To introduce testing of Grapevine leafroll-associated virus-2 (GLRaV-2) and Grapevine virus B (GVB) in sanitary selection of grapevine, commercially available antibodies were evaluated and conditions for routine ELISA testing were optimized. Extraction procedure with Granex 91 - special machine, which is used in routine testing in Slovenia, was compared with grinding samples in mortar. Three different extraction buffers were applied in order to overcome the inconvenience of using more than one extraction procedure when testing grapevine material for several viruses in a routine large-scale testing scheme. Results were verified with Western blot and immuno-electron microscopy. The best results were obtained using extraction buffer with unknown composition (pH 9.0) from BIOREBA kit for GLRaV-2. Other extraction buffers gave less positive samples and they are not convenient for routine testing where extraction with Granex is done. Both viruses, GLRaV-2 and GVB were found in Slovenia, but they couldn't be correlated with rougose wood disease that appears on indigenous cultivar Refošk grafted on 'SO4' from collection vineyard in Komen. https://journals.uni-lj.si/aas/article/view/15169grapevineselectionGrapevine virus BGrapevine leafroll-associated virus-2 |
| spellingShingle | Irma TOMAŽIČ Irena MAVRIČ PLEŠKO Nataša PETROVIČ Maja RAVNIKAR Zora KOROŠEC-KORUZA Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine Acta Agriculturae Slovenica grapevine selection Grapevine virus B Grapevine leafroll-associated virus-2 |
| title | Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine |
| title_full | Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine |
| title_fullStr | Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine |
| title_full_unstemmed | Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine |
| title_short | Introduction of Grapevine virus B and Grapevine leafroll-associated virus 2 testing in sanitary selection of grapevine |
| title_sort | introduction of grapevine virus b and grapevine leafroll associated virus 2 testing in sanitary selection of grapevine |
| topic | grapevine selection Grapevine virus B Grapevine leafroll-associated virus-2 |
| url | https://journals.uni-lj.si/aas/article/view/15169 |
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