Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis
Background/Aims: Angiotensin II (Ang II) mediated signaling plays a key role in the development of chronic kidney damage that contributes to renal fibrosis. However, the gene expression changes regulated by Ang II in the early stage of acute renal injury remain unclear. Methods: C57BL/6 wild-type (W...
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Cell Physiol Biochem Press GmbH & Co KG
2014-09-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/366327 |
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| author | Jing-Si Zhang Yun-Long Zhang Hong-Xia Wang Yun-Long Xia Lei Wang Yi-Nong Jiang Hui-Hua Li Ying Liu |
| author_facet | Jing-Si Zhang Yun-Long Zhang Hong-Xia Wang Yun-Long Xia Lei Wang Yi-Nong Jiang Hui-Hua Li Ying Liu |
| author_sort | Jing-Si Zhang |
| collection | DOAJ |
| description | Background/Aims: Angiotensin II (Ang II) mediated signaling plays a key role in the development of chronic kidney damage that contributes to renal fibrosis. However, the gene expression changes regulated by Ang II in the early stage of acute renal injury remain unclear. Methods: C57BL/6 wild-type (WT) mice were injected with Ang II (1500 ng/kg/min) for 1, 3 and 7 days. A time series analysis of microarrays was performed to evaluate Ang II-induced differentially gene expression in the kidneys. The data of gene expression in the kidney was further dissected by ANOVA analysis, gene expression profiles, gene network construction and quantitative real-time RT-PCR. Ang II-induced renal inflammation and fibrosis in mice were confirmed by pathological examination. Results: Our microarray data showed that a total of 1,511 differentially expressed genes were identified in the kidneys at 1, 3 and 7 days after Ang II infusion. These genes function in multiple biological processes, including response to stimuli, immune response, cell adhesion, metabolic process, kidney development, regulation of blood pressure, and ion transport, which may play critical roles in the pathobiology of Ang II-induced acute renal injury at the early stage. Furthermore, among these genes, 20 genes were further selected for final investigation. The dynamic gene network analysis demonstrated that fatty acid binding protein 1 (Fabp1) localized in the core of the network. Conclusions: Our data suggests that genes involved in lipid metabolic process, especially Fabp1, may play a central role in the development of Ang II-induced acute renal injury at the early stage. |
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| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-11T08:16:51Z |
| publishDate | 2014-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
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| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-fc1f8596d76640fa99520b563ae1cb1e2022-12-22T01:14:46ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-09-013441137115110.1159/000366327366327Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network AnalysisJing-Si ZhangYun-Long ZhangHong-Xia WangYun-Long XiaLei WangYi-Nong JiangHui-Hua LiYing LiuBackground/Aims: Angiotensin II (Ang II) mediated signaling plays a key role in the development of chronic kidney damage that contributes to renal fibrosis. However, the gene expression changes regulated by Ang II in the early stage of acute renal injury remain unclear. Methods: C57BL/6 wild-type (WT) mice were injected with Ang II (1500 ng/kg/min) for 1, 3 and 7 days. A time series analysis of microarrays was performed to evaluate Ang II-induced differentially gene expression in the kidneys. The data of gene expression in the kidney was further dissected by ANOVA analysis, gene expression profiles, gene network construction and quantitative real-time RT-PCR. Ang II-induced renal inflammation and fibrosis in mice were confirmed by pathological examination. Results: Our microarray data showed that a total of 1,511 differentially expressed genes were identified in the kidneys at 1, 3 and 7 days after Ang II infusion. These genes function in multiple biological processes, including response to stimuli, immune response, cell adhesion, metabolic process, kidney development, regulation of blood pressure, and ion transport, which may play critical roles in the pathobiology of Ang II-induced acute renal injury at the early stage. Furthermore, among these genes, 20 genes were further selected for final investigation. The dynamic gene network analysis demonstrated that fatty acid binding protein 1 (Fabp1) localized in the core of the network. Conclusions: Our data suggests that genes involved in lipid metabolic process, especially Fabp1, may play a central role in the development of Ang II-induced acute renal injury at the early stage.http://www.karger.com/Article/FullText/366327Fabp1Angiotensin IIRenal damageMicroarrayGene expression profiles |
| spellingShingle | Jing-Si Zhang Yun-Long Zhang Hong-Xia Wang Yun-Long Xia Lei Wang Yi-Nong Jiang Hui-Hua Li Ying Liu Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis Cellular Physiology and Biochemistry Fabp1 Angiotensin II Renal damage Microarray Gene expression profiles |
| title | Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis |
| title_full | Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis |
| title_fullStr | Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis |
| title_full_unstemmed | Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis |
| title_short | Identification of Genes Related to the Early Stage of Angiotensin II-induced Acute Renal Injury by Microarray and Integrated Gene Network Analysis |
| title_sort | identification of genes related to the early stage of angiotensin ii induced acute renal injury by microarray and integrated gene network analysis |
| topic | Fabp1 Angiotensin II Renal damage Microarray Gene expression profiles |
| url | http://www.karger.com/Article/FullText/366327 |
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