Development of a confinable gene drive system in the human disease vector Aedes aegypti
Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importan...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-01-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/51701 |
| _version_ | 1811202809756909568 |
|---|---|
| author | Ming Li Ting Yang Nikolay P Kandul Michelle Bui Stephanie Gamez Robyn Raban Jared Bennett Héctor M Sánchez C Gregory C Lanzaro Hanno Schmidt Yoosook Lee John M Marshall Omar S Akbari |
| author_facet | Ming Li Ting Yang Nikolay P Kandul Michelle Bui Stephanie Gamez Robyn Raban Jared Bennett Héctor M Sánchez C Gregory C Lanzaro Hanno Schmidt Yoosook Lee John M Marshall Omar S Akbari |
| author_sort | Ming Li |
| collection | DOAJ |
| description | Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in Ae. aegypti. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of Ae. aegypti to combat local pathogen transmission. |
| first_indexed | 2024-04-12T02:44:50Z |
| format | Article |
| id | doaj.art-fc222d9e827b49deb09a576029f2d8cf |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:44:50Z |
| publishDate | 2020-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fc222d9e827b49deb09a576029f2d8cf2022-12-22T03:51:14ZengeLife Sciences Publications LtdeLife2050-084X2020-01-01910.7554/eLife.51701Development of a confinable gene drive system in the human disease vector Aedes aegyptiMing Li0https://orcid.org/0000-0002-7578-4968Ting Yang1https://orcid.org/0000-0001-7201-4231Nikolay P Kandul2Michelle Bui3Stephanie Gamez4Robyn Raban5https://orcid.org/0000-0002-5648-6770Jared Bennett6Héctor M Sánchez C7Gregory C Lanzaro8Hanno Schmidt9https://orcid.org/0000-0001-8915-891XYoosook Lee10John M Marshall11https://orcid.org/0000-0003-0603-7341Omar S Akbari12https://orcid.org/0000-0002-6853-9884Section of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United StatesDepartment of Biophysics, University of California, Berkeley, Berkeley, United StatesDivision of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, Berkeley, United StatesVector Genetics Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, United StatesVector Genetics Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, United StatesVector Genetics Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, United StatesDivision of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, Berkeley, United States; Innovative Genomics Institute, Berkeley, United StatesSection of Cell and Developmental Biology, University of California, San Diego, San Diego, United States; Tata Institute for Genetics and Society, University of California, San Diego, La Jolla, United StatesAedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in Ae. aegypti. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of Ae. aegypti to combat local pathogen transmission.https://elifesciences.org/articles/51701Aedes aegyptiCRISPRsplit gene drivesCas9dengue |
| spellingShingle | Ming Li Ting Yang Nikolay P Kandul Michelle Bui Stephanie Gamez Robyn Raban Jared Bennett Héctor M Sánchez C Gregory C Lanzaro Hanno Schmidt Yoosook Lee John M Marshall Omar S Akbari Development of a confinable gene drive system in the human disease vector Aedes aegypti eLife Aedes aegypti CRISPR split gene drives Cas9 dengue |
| title | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
| title_full | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
| title_fullStr | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
| title_full_unstemmed | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
| title_short | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
| title_sort | development of a confinable gene drive system in the human disease vector aedes aegypti |
| topic | Aedes aegypti CRISPR split gene drives Cas9 dengue |
| url | https://elifesciences.org/articles/51701 |
| work_keys_str_mv | AT mingli developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT tingyang developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT nikolaypkandul developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT michellebui developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT stephaniegamez developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT robynraban developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT jaredbennett developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT hectormsanchezc developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT gregoryclanzaro developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT hannoschmidt developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT yoosooklee developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT johnmmarshall developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti AT omarsakbari developmentofaconfinablegenedrivesysteminthehumandiseasevectoraedesaegypti |