Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol
Wheat flour liquid waste (WF-LW) is a potential biomass that is chemically pre-treated before being enzymatically hydrolyzed to produce glucose-derived bioethanol. This study investigated the effects of pH on the pretreatment process and the subsequent hydrolysis of WF-LW in the presence of amylase...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Cogent Engineering |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23311916.2022.2101229 |
| _version_ | 1827833630463885312 |
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| author | N.K. Sari I.Y. Purbasari P.W. Anggoro J. Jamari A.P. Bayuseno |
| author_facet | N.K. Sari I.Y. Purbasari P.W. Anggoro J. Jamari A.P. Bayuseno |
| author_sort | N.K. Sari |
| collection | DOAJ |
| description | Wheat flour liquid waste (WF-LW) is a potential biomass that is chemically pre-treated before being enzymatically hydrolyzed to produce glucose-derived bioethanol. This study investigated the effects of pH on the pretreatment process and the subsequent hydrolysis of WF-LW in the presence of amylase (AE) and maltose enzymes (ME). In the experiment, WF-LW at varying volumes from 200 to 1000 mL was treated with 5 to 25 mL of HCl and stirred for 30 minutes and at the temperature of 40°C. Impurities were removed prior to hydrolysis. Following that, a hydrolysis with an AE of 11% w/v and ME of 9% w/v resulted in a glucose content of 24.9% v/v. Moreover, 0.859 mL of HCl was added to achieve the best results, which were then optimized using SRM (surface response methodology) at a pH of 8.85 in a WF-LW volume of 1165.69 mL. The optimization of the pH and fermentation process in the presence of amylase and maltase enzymes resulted in increased glucose production and a decrease in the LW-TW treatment volume, which was 651.426 mL. These discoveries would become an appropriate approach for the subsequent fermentation process, resulting in the highest glucose levels possible. |
| first_indexed | 2024-03-12T05:35:01Z |
| format | Article |
| id | doaj.art-fc23778510b94219aa5c04c3591cb97e |
| institution | Directory Open Access Journal |
| issn | 2331-1916 |
| language | English |
| last_indexed | 2024-03-12T05:35:01Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Engineering |
| spelling | doaj.art-fc23778510b94219aa5c04c3591cb97e2023-09-03T06:32:29ZengTaylor & Francis GroupCogent Engineering2331-19162022-12-019110.1080/23311916.2022.2101229Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanolN.K. Sari0I.Y. Purbasari1P.W. Anggoro2J. Jamari3A.P. Bayuseno4Department of Chemical Engineering, Faculty of Technology, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Jl. Rungkut Madya No. 1, Gn. Anyar, Kec. Gn. Anyar, Kota SBY, Jawa Timur 60294, IndonesiaDepartment of Informatics, Faculty of Computer Science, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Jl. Rungkut Madya No. 1, Gn. Anyar, Kec. Gn. Anyar, Kota SBY, Jawa Timur 60294, IndonesiaDepartment of Industrial Engineering, Faculty of Industrial Technology, University of Atma Jaya Yogyakarta, Jl. Babarsari 44, Yogyakarta 55281, IndonesiaDepartment of Mechanical Engineering, University of Diponegoro, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50275, IndonesiaDepartment of Mechanical Engineering, University of Diponegoro, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50275, IndonesiaWheat flour liquid waste (WF-LW) is a potential biomass that is chemically pre-treated before being enzymatically hydrolyzed to produce glucose-derived bioethanol. This study investigated the effects of pH on the pretreatment process and the subsequent hydrolysis of WF-LW in the presence of amylase (AE) and maltose enzymes (ME). In the experiment, WF-LW at varying volumes from 200 to 1000 mL was treated with 5 to 25 mL of HCl and stirred for 30 minutes and at the temperature of 40°C. Impurities were removed prior to hydrolysis. Following that, a hydrolysis with an AE of 11% w/v and ME of 9% w/v resulted in a glucose content of 24.9% v/v. Moreover, 0.859 mL of HCl was added to achieve the best results, which were then optimized using SRM (surface response methodology) at a pH of 8.85 in a WF-LW volume of 1165.69 mL. The optimization of the pH and fermentation process in the presence of amylase and maltase enzymes resulted in increased glucose production and a decrease in the LW-TW treatment volume, which was 651.426 mL. These discoveries would become an appropriate approach for the subsequent fermentation process, resulting in the highest glucose levels possible.https://www.tandfonline.com/doi/10.1080/23311916.2022.2101229wheat flour liquid wastepHamylase and maltose enzymessurface response methodology (SRM) |
| spellingShingle | N.K. Sari I.Y. Purbasari P.W. Anggoro J. Jamari A.P. Bayuseno Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol Cogent Engineering wheat flour liquid waste pH amylase and maltose enzymes surface response methodology (SRM) |
| title | Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol |
| title_full | Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol |
| title_fullStr | Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol |
| title_full_unstemmed | Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol |
| title_short | Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol |
| title_sort | reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose derived bioethanol |
| topic | wheat flour liquid waste pH amylase and maltose enzymes surface response methodology (SRM) |
| url | https://www.tandfonline.com/doi/10.1080/23311916.2022.2101229 |
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