Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model
Cardiac pumping depends on the morphological structure of the heart, but also on its subcellular (ultrastructural) architecture, which enables cardiac contraction. In cases of congenital heart defects, localized ultrastructural disruptions that increase the risk of heart failure are only starting to...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/58138 |
| _version_ | 1828380224538017792 |
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| author | Graham Rykiel Claudia S López Jessica L Riesterer Ian Fries Sanika Deosthali Katherine Courchaine Alina Maloyan Kent Thornburg Sandra Rugonyi |
| author_facet | Graham Rykiel Claudia S López Jessica L Riesterer Ian Fries Sanika Deosthali Katherine Courchaine Alina Maloyan Kent Thornburg Sandra Rugonyi |
| author_sort | Graham Rykiel |
| collection | DOAJ |
| description | Cardiac pumping depends on the morphological structure of the heart, but also on its subcellular (ultrastructural) architecture, which enables cardiac contraction. In cases of congenital heart defects, localized ultrastructural disruptions that increase the risk of heart failure are only starting to be discovered. This is in part due to a lack of technologies that can image the three-dimensional (3D) heart structure, to assess malformations; and its ultrastructure, to assess organelle disruptions. We present here a multiscale, correlative imaging procedure that achieves high-resolution images of the whole heart, using 3D micro-computed tomography (micro-CT); and its ultrastructure, using 3D scanning electron microscopy (SEM). In a small animal model (chicken embryo), we achieved uniform fixation and staining of the whole heart, without losing ultrastructural preservation on the same sample, enabling correlative multiscale imaging. Our approach enables multiscale studies in models of congenital heart disease and beyond. |
| first_indexed | 2024-12-10T03:53:35Z |
| format | Article |
| id | doaj.art-fc24e5a5d945488fb19bae519e84c0af |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:53:35Z |
| publishDate | 2020-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fc24e5a5d945488fb19bae519e84c0af2022-12-22T02:03:11ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.58138Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal modelGraham Rykiel0Claudia S López1Jessica L Riesterer2https://orcid.org/0000-0003-1084-2773Ian Fries3Sanika Deosthali4Katherine Courchaine5Alina Maloyan6https://orcid.org/0000-0002-7309-5026Kent Thornburg7Sandra Rugonyi8https://orcid.org/0000-0001-9262-7959Biomedical Engineering, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United States; Multiscale Microscopy Core, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United States; Multiscale Microscopy Core, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United StatesCenter for Developmental Health, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, United StatesCenter for Developmental Health, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, United StatesBiomedical Engineering, Oregon Health & Science University, Portland, United States; Center for Developmental Health, Knight Cardiovascular Institute, Oregon Health & Science University, Portland, United StatesCardiac pumping depends on the morphological structure of the heart, but also on its subcellular (ultrastructural) architecture, which enables cardiac contraction. In cases of congenital heart defects, localized ultrastructural disruptions that increase the risk of heart failure are only starting to be discovered. This is in part due to a lack of technologies that can image the three-dimensional (3D) heart structure, to assess malformations; and its ultrastructure, to assess organelle disruptions. We present here a multiscale, correlative imaging procedure that achieves high-resolution images of the whole heart, using 3D micro-computed tomography (micro-CT); and its ultrastructure, using 3D scanning electron microscopy (SEM). In a small animal model (chicken embryo), we achieved uniform fixation and staining of the whole heart, without losing ultrastructural preservation on the same sample, enabling correlative multiscale imaging. Our approach enables multiscale studies in models of congenital heart disease and beyond.https://elifesciences.org/articles/58138heart defectsfetal heart structurefetal heart ultrastructuremyocardial organizationcongenital defects |
| spellingShingle | Graham Rykiel Claudia S López Jessica L Riesterer Ian Fries Sanika Deosthali Katherine Courchaine Alina Maloyan Kent Thornburg Sandra Rugonyi Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model eLife heart defects fetal heart structure fetal heart ultrastructure myocardial organization congenital defects |
| title | Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| title_full | Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| title_fullStr | Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| title_full_unstemmed | Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| title_short | Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| title_sort | multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model |
| topic | heart defects fetal heart structure fetal heart ultrastructure myocardial organization congenital defects |
| url | https://elifesciences.org/articles/58138 |
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