Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review
Organoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not o...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/6/379 |
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| author | Zheng Li Muxin Yue Yunsong Liu Ping Zhang Jia Qing Hao Liu Yongsheng Zhou |
| author_facet | Zheng Li Muxin Yue Yunsong Liu Ping Zhang Jia Qing Hao Liu Yongsheng Zhou |
| author_sort | Zheng Li |
| collection | DOAJ |
| description | Organoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not only recapitulate the histological and genetic characteristics of organs in vivo, but also form tissues with the capacity for self-renewal and further differentiation. Recent advances in biomaterial technology, particularly hydrogels, have provided opportunities to improve organoid cultures; by closely integrating the mechanical and chemical properties of the extracellular matrix microenvironment, with novel synthetic materials and stem cell biology. This systematic review critically examines recent advances in various strategies and techniques utilized for stem-cell-derived organoid culture, with particular emphasis on the application potential of hydrogel technology in organoid culture. We hope this will give a better understanding of organoid cultures for modelling diseases and tissue engineering applications. |
| first_indexed | 2024-03-09T23:45:21Z |
| format | Article |
| id | doaj.art-fc24f5887bba4901ae114910f04479fc |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T23:45:21Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-fc24f5887bba4901ae114910f04479fc2023-11-23T16:45:50ZengMDPI AGGels2310-28612022-06-018637910.3390/gels8060379Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic ReviewZheng Li0Muxin Yue1Yunsong Liu2Ping Zhang3Jia Qing4Hao Liu5Yongsheng Zhou6Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaThe Central Laboratory, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaOrganoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not only recapitulate the histological and genetic characteristics of organs in vivo, but also form tissues with the capacity for self-renewal and further differentiation. Recent advances in biomaterial technology, particularly hydrogels, have provided opportunities to improve organoid cultures; by closely integrating the mechanical and chemical properties of the extracellular matrix microenvironment, with novel synthetic materials and stem cell biology. This systematic review critically examines recent advances in various strategies and techniques utilized for stem-cell-derived organoid culture, with particular emphasis on the application potential of hydrogel technology in organoid culture. We hope this will give a better understanding of organoid cultures for modelling diseases and tissue engineering applications.https://www.mdpi.com/2310-2861/8/6/379organoidhydrogelstem cellstissue engineering |
| spellingShingle | Zheng Li Muxin Yue Yunsong Liu Ping Zhang Jia Qing Hao Liu Yongsheng Zhou Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review Gels organoid hydrogel stem cells tissue engineering |
| title | Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review |
| title_full | Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review |
| title_fullStr | Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review |
| title_full_unstemmed | Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review |
| title_short | Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review |
| title_sort | advances of engineered hydrogel organoids within the stem cell field a systematic review |
| topic | organoid hydrogel stem cells tissue engineering |
| url | https://www.mdpi.com/2310-2861/8/6/379 |
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