Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
In order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluate...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
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Universidad Central Marta Abreu de Las Villas
2007-01-01
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Series: | Biotecnología Vegetal |
Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/354 |
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author | Raúl Collado Raúl Barbón Daniel Agramonte Felipe Jiménez-Terry Martha Pérez Odalys Gutiérrez Mariana La O |
author_facet | Raúl Collado Raúl Barbón Daniel Agramonte Felipe Jiménez-Terry Martha Pérez Odalys Gutiérrez Mariana La O |
author_sort | Raúl Collado |
collection | DOAJ |
description | In order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluated in independent experiments. After 60 days of culture the number of somatic embryos that remained alive, germinated and showed secondary embryogenesis was quantified. It was verified that the germination of the somatic embryos was affected with the dehydration process; nevertheless, the culture of the embryos during 30 days in culture medium with 4.0 and 6.0% of sucrose and later to transfer them to germination medium increased the number of germinated embryos without affecting the survival and with normal development of bud and root.
Key words: dehydration, germination, osmotic agent, somatic embryos |
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institution | Directory Open Access Journal |
issn | 1609-1841 2074-8647 |
language | English |
last_indexed | 2024-12-10T15:30:21Z |
publishDate | 2007-01-01 |
publisher | Universidad Central Marta Abreu de Las Villas |
record_format | Article |
series | Biotecnología Vegetal |
spelling | doaj.art-fc26bf5552bc484fa74423e58a8f56e22022-12-22T01:43:25ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-01-0171327Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germinationRaúl ColladoRaúl BarbónDaniel AgramonteFelipe Jiménez-TerryMartha PérezOdalys GutiérrezMariana La OIn order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluated in independent experiments. After 60 days of culture the number of somatic embryos that remained alive, germinated and showed secondary embryogenesis was quantified. It was verified that the germination of the somatic embryos was affected with the dehydration process; nevertheless, the culture of the embryos during 30 days in culture medium with 4.0 and 6.0% of sucrose and later to transfer them to germination medium increased the number of germinated embryos without affecting the survival and with normal development of bud and root. Key words: dehydration, germination, osmotic agent, somatic embryoshttps://revista.ibp.co.cu/index.php/BV/article/view/354 |
spellingShingle | Raúl Collado Raúl Barbón Daniel Agramonte Felipe Jiménez-Terry Martha Pérez Odalys Gutiérrez Mariana La O Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination Biotecnología Vegetal |
title | Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination |
title_full | Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination |
title_fullStr | Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination |
title_full_unstemmed | Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination |
title_short | Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination |
title_sort | effect of the dehydration and sucrose on em swietenia macrophylla em king somatic embryos germination somatic embryos germination |
url | https://revista.ibp.co.cu/index.php/BV/article/view/354 |
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