Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination

In order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluate...

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Main Authors: Raúl Collado, Raúl Barbón, Daniel Agramonte, Felipe Jiménez-Terry, Martha Pérez, Odalys Gutiérrez, Mariana La O
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2007-01-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/354
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author Raúl Collado
Raúl Barbón
Daniel Agramonte
Felipe Jiménez-Terry
Martha Pérez
Odalys Gutiérrez
Mariana La O
author_facet Raúl Collado
Raúl Barbón
Daniel Agramonte
Felipe Jiménez-Terry
Martha Pérez
Odalys Gutiérrez
Mariana La O
author_sort Raúl Collado
collection DOAJ
description In order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluated in independent experiments. After 60 days of culture the number of somatic embryos that remained alive, germinated and showed secondary embryogenesis was quantified. It was verified that the germination of the somatic embryos was affected with the dehydration process; nevertheless, the culture of the embryos during 30 days in culture medium with 4.0 and 6.0% of sucrose and later to transfer them to germination medium increased the number of germinated embryos without affecting the survival and with normal development of bud and root. Key words: dehydration, germination, osmotic agent, somatic embryos
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2074-8647
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spelling doaj.art-fc26bf5552bc484fa74423e58a8f56e22022-12-22T01:43:25ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-01-0171327Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germinationRaúl ColladoRaúl BarbónDaniel AgramonteFelipe Jiménez-TerryMartha PérezOdalys GutiérrezMariana La OIn order to improve the germination of somatic embryos of Swietenia macrophylla the effect of the dehydration and the increase of sucrose concentration in culture medium were evaluated. Three times of dehydration (48, 72 and 96 hours) and three sucrose concentration (4.0, 6.0 and 8.0%) were evaluated in independent experiments. After 60 days of culture the number of somatic embryos that remained alive, germinated and showed secondary embryogenesis was quantified. It was verified that the germination of the somatic embryos was affected with the dehydration process; nevertheless, the culture of the embryos during 30 days in culture medium with 4.0 and 6.0% of sucrose and later to transfer them to germination medium increased the number of germinated embryos without affecting the survival and with normal development of bud and root. Key words: dehydration, germination, osmotic agent, somatic embryoshttps://revista.ibp.co.cu/index.php/BV/article/view/354
spellingShingle Raúl Collado
Raúl Barbón
Daniel Agramonte
Felipe Jiménez-Terry
Martha Pérez
Odalys Gutiérrez
Mariana La O
Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
Biotecnología Vegetal
title Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
title_full Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
title_fullStr Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
title_full_unstemmed Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
title_short Effect of the dehydration and sucrose on <em>Swietenia macrophylla</em> King somatic embryos germination somatic embryos germination
title_sort effect of the dehydration and sucrose on em swietenia macrophylla em king somatic embryos germination somatic embryos germination
url https://revista.ibp.co.cu/index.php/BV/article/view/354
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