Test system creation for analysis of template switching effect during a reverse transcription <i>in vitro</i>

We investigated an in vitro system for the analysis of template switching effect of RNA-dependent DNA-polymerase during reverse transcription reaction. An RT-PCR-based approach was realized. An RNA sample was used as a template. The RNA contains a region with a stable secondary structure flanked by...

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Bibliographic Details
Main Authors: A. Y. Komkov, Y. B. Lebedev, V. I. Shvets
Format: Article
Language:Russian
Published: MIREA - Russian Technological University 2011-12-01
Series:Тонкие химические технологии
Subjects:
Online Access:https://www.finechem-mirea.ru/jour/article/view/1191

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