<i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies
Glomerular diseases (GNs) are responsible for approximately 20% of chronic kidney diseases. Glucocorticoid receptor gene (<i>NR3C1</i>) single nucleotide polymorphisms (SNPs) are implicated in differences in predisposition to autoimmunity and steroid sensitivity. The aim of this study wa...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/11/3186 |
| _version_ | 1827677061361172480 |
|---|---|
| author | Michał Pac Natalia Krata Barbara Moszczuk Aleksandra Wyczałkowska-Tomasik Beata Kaleta Bartosz Foroncewicz Witold Rudnicki Leszek Pączek Krzysztof Mucha |
| author_facet | Michał Pac Natalia Krata Barbara Moszczuk Aleksandra Wyczałkowska-Tomasik Beata Kaleta Bartosz Foroncewicz Witold Rudnicki Leszek Pączek Krzysztof Mucha |
| author_sort | Michał Pac |
| collection | DOAJ |
| description | Glomerular diseases (GNs) are responsible for approximately 20% of chronic kidney diseases. Glucocorticoid receptor gene (<i>NR3C1</i>) single nucleotide polymorphisms (SNPs) are implicated in differences in predisposition to autoimmunity and steroid sensitivity. The aim of this study was to evaluate the frequency of the <i>NR3C1</i> SNPs—rs6198, rs41423247 and rs17209237—in 72 IgA nephropathy (IgAN) and 38 membranous nephropathy (MN) patients compared to 175 healthy controls and to correlate the effectiveness of treatment in IgAN and MN groups defined as a reduction of proteinuria <1 g/24 h after 12 months of treatment. Real-time polymerase chain reactions and SNP array-based typing were used. We found significant rs41423247 association with MN (<i>p</i> = 0.026); a significant association of rs17209237 with eGFR reduction after follow-up period in all patients with GNs (<i>p</i> = 0.021) and with the degree of proteinuria after 1 year of therapy in all patients with a glomerulopathy (<i>p</i> = 0.013) and IgAN (<i>p</i> = 0.021); and in the same groups treated with steroids (<i>p</i> = 0.021; <i>p</i> = 0.012). We also observed the association between rs41423247 and IgAN histopathologic findings (<i>p</i> = 0.012). In conclusion, our results indicate that <i>NR3C1</i> polymorphisms may influence treatment susceptibility and clinical outcome in IgAN and MN. |
| first_indexed | 2024-03-10T05:36:56Z |
| format | Article |
| id | doaj.art-fc344cdf1a3d439d882e7ba6bc08a608 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:36:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-fc344cdf1a3d439d882e7ba6bc08a6082023-11-22T22:52:34ZengMDPI AGCells2073-44092021-11-011011318610.3390/cells10113186<i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA NephropathiesMichał Pac0Natalia Krata1Barbara Moszczuk2Aleksandra Wyczałkowska-Tomasik3Beata Kaleta4Bartosz Foroncewicz5Witold Rudnicki6Leszek Pączek7Krzysztof Mucha8Department of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandComputational Centre and Institute of Computer Science, University of Białystok, 15-245 Białystok, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, 02-006 Warsaw, PolandGlomerular diseases (GNs) are responsible for approximately 20% of chronic kidney diseases. Glucocorticoid receptor gene (<i>NR3C1</i>) single nucleotide polymorphisms (SNPs) are implicated in differences in predisposition to autoimmunity and steroid sensitivity. The aim of this study was to evaluate the frequency of the <i>NR3C1</i> SNPs—rs6198, rs41423247 and rs17209237—in 72 IgA nephropathy (IgAN) and 38 membranous nephropathy (MN) patients compared to 175 healthy controls and to correlate the effectiveness of treatment in IgAN and MN groups defined as a reduction of proteinuria <1 g/24 h after 12 months of treatment. Real-time polymerase chain reactions and SNP array-based typing were used. We found significant rs41423247 association with MN (<i>p</i> = 0.026); a significant association of rs17209237 with eGFR reduction after follow-up period in all patients with GNs (<i>p</i> = 0.021) and with the degree of proteinuria after 1 year of therapy in all patients with a glomerulopathy (<i>p</i> = 0.013) and IgAN (<i>p</i> = 0.021); and in the same groups treated with steroids (<i>p</i> = 0.021; <i>p</i> = 0.012). We also observed the association between rs41423247 and IgAN histopathologic findings (<i>p</i> = 0.012). In conclusion, our results indicate that <i>NR3C1</i> polymorphisms may influence treatment susceptibility and clinical outcome in IgAN and MN.https://www.mdpi.com/2073-4409/10/11/3186genomicsglucocorticoid receptorIgA nephropathymembranous nephropathysingle nucleotide polymorphism |
| spellingShingle | Michał Pac Natalia Krata Barbara Moszczuk Aleksandra Wyczałkowska-Tomasik Beata Kaleta Bartosz Foroncewicz Witold Rudnicki Leszek Pączek Krzysztof Mucha <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies Cells genomics glucocorticoid receptor IgA nephropathy membranous nephropathy single nucleotide polymorphism |
| title | <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies |
| title_full | <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies |
| title_fullStr | <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies |
| title_full_unstemmed | <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies |
| title_short | <i>NR3C1</i> Glucocorticoid Receptor Gene Polymorphisms Are Associated with Membranous and IgA Nephropathies |
| title_sort | i nr3c1 i glucocorticoid receptor gene polymorphisms are associated with membranous and iga nephropathies |
| topic | genomics glucocorticoid receptor IgA nephropathy membranous nephropathy single nucleotide polymorphism |
| url | https://www.mdpi.com/2073-4409/10/11/3186 |
| work_keys_str_mv | AT michałpac inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT nataliakrata inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT barbaramoszczuk inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT aleksandrawyczałkowskatomasik inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT beatakaleta inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT bartoszforoncewicz inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT witoldrudnicki inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT leszekpaczek inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies AT krzysztofmucha inr3c1iglucocorticoidreceptorgenepolymorphismsareassociatedwithmembranousandiganephropathies |