Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages
Expression level of Tumor Necrosis Factor—related apoptosis—inducing ligand (TRAIL) receptors is one of the most important factors of TRAIL-mediated apoptosis in cancer cells. We here report for the first time data concerning TRAIL-R1 and TRAIL-R2 receptor expression on RAW264.7 macrophages. Three s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/15/7/11510 |
| _version_ | 1811204409771687936 |
|---|---|
| author | Monika Warat Ewelina Szliszka Ilona Korzonek-Szlacheta Wojciech Król Zenon P. Czuba |
| author_facet | Monika Warat Ewelina Szliszka Ilona Korzonek-Szlacheta Wojciech Król Zenon P. Czuba |
| author_sort | Monika Warat |
| collection | DOAJ |
| description | Expression level of Tumor Necrosis Factor—related apoptosis—inducing ligand (TRAIL) receptors is one of the most important factors of TRAIL-mediated apoptosis in cancer cells. We here report for the first time data concerning TRAIL-R1 and TRAIL-R2 receptor expression on RAW264.7 macrophages. Three substances belonging to flavones: chrysin, apigenin and acacetin which differ from their substituents at the 4' position in the phenyl ring were used in assays because of the variety of biological activities (e.g., anticancer activity) of the polyphenol compounds. The expression of TRAIL-R1 and TRAIL-R2 death receptors on non-stimulated and LPS (lipopolysaccharide)-stimulated macrophages was determined using flow cytometry. We demonstrate that RAW264.7 macrophages exhibit TRAIL-R1 surface expression and that the tested compounds: chrysin, apigenin and acacetin can inhibit TRAIL-R1 death receptor expression level on macrophages. |
| first_indexed | 2024-04-12T03:13:48Z |
| format | Article |
| id | doaj.art-fc35ce719ac247bbbdbf3e25ce25180a |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T03:13:48Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fc35ce719ac247bbbdbf3e25ce25180a2022-12-22T03:50:17ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-06-01157115101152210.3390/ijms150711510ijms150711510Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 MacrophagesMonika Warat0Ewelina Szliszka1Ilona Korzonek-Szlacheta2Wojciech Król3Zenon P. Czuba4Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, PolandChair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, PolandDepartment of Toxicology and Health Protection, Toxicology and Drug Addiction Division, Medical University of Silesia in Katowice, Medyków 18, 40-752 Katowice, PolandChair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, PolandChair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, PolandExpression level of Tumor Necrosis Factor—related apoptosis—inducing ligand (TRAIL) receptors is one of the most important factors of TRAIL-mediated apoptosis in cancer cells. We here report for the first time data concerning TRAIL-R1 and TRAIL-R2 receptor expression on RAW264.7 macrophages. Three substances belonging to flavones: chrysin, apigenin and acacetin which differ from their substituents at the 4' position in the phenyl ring were used in assays because of the variety of biological activities (e.g., anticancer activity) of the polyphenol compounds. The expression of TRAIL-R1 and TRAIL-R2 death receptors on non-stimulated and LPS (lipopolysaccharide)-stimulated macrophages was determined using flow cytometry. We demonstrate that RAW264.7 macrophages exhibit TRAIL-R1 surface expression and that the tested compounds: chrysin, apigenin and acacetin can inhibit TRAIL-R1 death receptor expression level on macrophages.http://www.mdpi.com/1422-0067/15/7/11510chrysinapigeninacacetinTRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-receptor (TRAIL-R) expressionRAW264.7 |
| spellingShingle | Monika Warat Ewelina Szliszka Ilona Korzonek-Szlacheta Wojciech Król Zenon P. Czuba Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages International Journal of Molecular Sciences chrysin apigenin acacetin TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-receptor (TRAIL-R) expression RAW264.7 |
| title | Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages |
| title_full | Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages |
| title_fullStr | Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages |
| title_full_unstemmed | Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages |
| title_short | Chrysin, Apigenin and Acacetin Inhibit Tumor Necrosis Factor-Related Apoptosis—Inducing Ligand Receptor-1 (TRAIL-R1) on Activated RAW264.7 Macrophages |
| title_sort | chrysin apigenin and acacetin inhibit tumor necrosis factor related apoptosis inducing ligand receptor 1 trail r1 on activated raw264 7 macrophages |
| topic | chrysin apigenin acacetin TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-receptor (TRAIL-R) expression RAW264.7 |
| url | http://www.mdpi.com/1422-0067/15/7/11510 |
| work_keys_str_mv | AT monikawarat chrysinapigeninandacacetininhibittumornecrosisfactorrelatedapoptosisinducingligandreceptor1trailr1onactivatedraw2647macrophages AT ewelinaszliszka chrysinapigeninandacacetininhibittumornecrosisfactorrelatedapoptosisinducingligandreceptor1trailr1onactivatedraw2647macrophages AT ilonakorzonekszlacheta chrysinapigeninandacacetininhibittumornecrosisfactorrelatedapoptosisinducingligandreceptor1trailr1onactivatedraw2647macrophages AT wojciechkrol chrysinapigeninandacacetininhibittumornecrosisfactorrelatedapoptosisinducingligandreceptor1trailr1onactivatedraw2647macrophages AT zenonpczuba chrysinapigeninandacacetininhibittumornecrosisfactorrelatedapoptosisinducingligandreceptor1trailr1onactivatedraw2647macrophages |