A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max
Soybean (Glycine max) is a staple crop and a major source of vegetable protein and vegetable oil. The growth of soybean is dramatically inhibited by salt stress, especially by the excessive toxic Na+. Salt Overly Sensitive 1 (SOS1) is the only extensively characterized Na+ efflux transporter in mult...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.870695/full |
| _version_ | 1818202120045723648 |
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| author | Minghui Zhang Junfeng Cao Tianxu Zhang Tianxu Zhang Tao Xu Liyuan Yang Liyuan Yang Xiaoyuan Li Fengdan Ji Yingxue Gao Shahid Ali Shahid Ali Qingzhu Zhang Qingzhu Zhang Jianhua Zhu Linan Xie |
| author_facet | Minghui Zhang Junfeng Cao Tianxu Zhang Tianxu Zhang Tao Xu Liyuan Yang Liyuan Yang Xiaoyuan Li Fengdan Ji Yingxue Gao Shahid Ali Shahid Ali Qingzhu Zhang Qingzhu Zhang Jianhua Zhu Linan Xie |
| author_sort | Minghui Zhang |
| collection | DOAJ |
| description | Soybean (Glycine max) is a staple crop and a major source of vegetable protein and vegetable oil. The growth of soybean is dramatically inhibited by salt stress, especially by the excessive toxic Na+. Salt Overly Sensitive 1 (SOS1) is the only extensively characterized Na+ efflux transporter in multiple plant species so far. However, the role of GmSOS1 in soybean salt stress responses remains unclear. Herein, we created three gmsos1 mutants using the CRISPR-Cas9 system in soybean. We found a significant accumulation of Na+ in the roots of the gmsos1 mutants, resulting in the imbalance of Na+ and K+, which links to impaired Na+ efflux and increased K+ efflux in the roots of the gmsos1 mutants under salt stress. Compared to the wild type, our RNA-seq analysis revealed that the roots of the gmsos1-1 showed preferential up and downregulation of ion transporters under salt stress, supporting impaired stress detection or an inability to develop a comprehensive response to salinity in the gmsos1 mutants. Our findings indicate that the plasma membrane Na+/H+ exchanger GmSOS1 plays a critical role in soybean salt tolerance by maintaining Na+ homeostasis and provides evidence for molecular breeding to improve salt tolerance in soybean and other crops. |
| first_indexed | 2024-12-12T03:04:23Z |
| format | Article |
| id | doaj.art-fc36d68b67b24182a9523aeeada3bcc6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T03:04:23Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fc36d68b67b24182a9523aeeada3bcc62022-12-22T00:40:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.870695870695A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine maxMinghui Zhang0Junfeng Cao1Tianxu Zhang2Tianxu Zhang3Tao Xu4Liyuan Yang5Liyuan Yang6Xiaoyuan Li7Fengdan Ji8Yingxue Gao9Shahid Ali10Shahid Ali11Qingzhu Zhang12Qingzhu Zhang13Jianhua Zhu14Linan Xie15Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaCollege of Life Science, Northeast Forestry University, Harbin, ChinaThe Editorial Board of Journal of Forestry Research, Northeast Forestry University, Harbin, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaLaboratory Department, Qitaihe Center for Disease Control and Prevention, Qitaihe, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaShanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaCollege of Life Science, Northeast Forestry University, Harbin, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, ChinaCollege of Life Science, Northeast Forestry University, Harbin, ChinaDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, College Park, MD, United StatesKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, ChinaSoybean (Glycine max) is a staple crop and a major source of vegetable protein and vegetable oil. The growth of soybean is dramatically inhibited by salt stress, especially by the excessive toxic Na+. Salt Overly Sensitive 1 (SOS1) is the only extensively characterized Na+ efflux transporter in multiple plant species so far. However, the role of GmSOS1 in soybean salt stress responses remains unclear. Herein, we created three gmsos1 mutants using the CRISPR-Cas9 system in soybean. We found a significant accumulation of Na+ in the roots of the gmsos1 mutants, resulting in the imbalance of Na+ and K+, which links to impaired Na+ efflux and increased K+ efflux in the roots of the gmsos1 mutants under salt stress. Compared to the wild type, our RNA-seq analysis revealed that the roots of the gmsos1-1 showed preferential up and downregulation of ion transporters under salt stress, supporting impaired stress detection or an inability to develop a comprehensive response to salinity in the gmsos1 mutants. Our findings indicate that the plasma membrane Na+/H+ exchanger GmSOS1 plays a critical role in soybean salt tolerance by maintaining Na+ homeostasis and provides evidence for molecular breeding to improve salt tolerance in soybean and other crops.https://www.frontiersin.org/articles/10.3389/fpls.2022.870695/fullGmSOS1soybeanNa+ effluxsalt tolerancebreeding |
| spellingShingle | Minghui Zhang Junfeng Cao Tianxu Zhang Tianxu Zhang Tao Xu Liyuan Yang Liyuan Yang Xiaoyuan Li Fengdan Ji Yingxue Gao Shahid Ali Shahid Ali Qingzhu Zhang Qingzhu Zhang Jianhua Zhu Linan Xie A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max Frontiers in Plant Science GmSOS1 soybean Na+ efflux salt tolerance breeding |
| title | A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max |
| title_full | A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max |
| title_fullStr | A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max |
| title_full_unstemmed | A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max |
| title_short | A Putative Plasma Membrane Na+/H+ Antiporter GmSOS1 Is Critical for Salt Stress Tolerance in Glycine max |
| title_sort | putative plasma membrane na h antiporter gmsos1 is critical for salt stress tolerance in glycine max |
| topic | GmSOS1 soybean Na+ efflux salt tolerance breeding |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.870695/full |
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