Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep
PCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism of mannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes were identified in intron1, which wer...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2019-02-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2481 |
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| author | Mengting ZHAI Jian MOU Mengting ZHU Yanping LIANG Mingyuan WANG Zongsheng ZHAO Hongmei ZHANG |
| author_facet | Mengting ZHAI Jian MOU Mengting ZHU Yanping LIANG Mingyuan WANG Zongsheng ZHAO Hongmei ZHANG |
| author_sort | Mengting ZHAI |
| collection | DOAJ |
| description | PCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism of
mannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes
were identified in intron1, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron2, which were controlled
by 2 alleles, respectively; 3 genotypes were identified in intron3, which were controlled by 2 alleles, respectively; 6 new single nucleotide
polymorphisms(SNPs) mutation sites were found, g.288T>A in intron1, g. 1091 T>C, g.1096A>C, g.1770G>C in intron 2, g.2297C>T, g.2331G>A
in intron3. Use ELISA to detect MBL serum level in Hu sheep, then use One-way ANOVA analyze the relationship of different genotypes and
MBL serum level. And the result showed that the MBL serum level of AA genotype was higher than BB genotype (P<0.05) in intron1, CC
genotype was higher than DD, GG genotype was higher than HH (P<0.05) in intron2. So, AA, CC and GG genotypes may be related to disease
resistance; BB, DD and HH genotypes be related to disease susceptibility. The point mutation in intron3 couldn"t lead to the change of MBL
serum level. |
| first_indexed | 2024-03-13T04:38:54Z |
| format | Article |
| id | doaj.art-fc37338af94543408142ec66c0c119a1 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:54Z |
| publishDate | 2019-02-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-fc37338af94543408142ec66c0c119a12023-06-19T06:34:31ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-02-0125334935610.9775/kvfd.2018.209692481Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheepMengting ZHAI0Jian MOU1Mengting ZHU2Yanping LIANG3Mingyuan WANG4Zongsheng ZHAO5Hongmei ZHANG6College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINACollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832003, PR CHINADepartment of Clinical Laboratory, First Affiliated Hospital, Shihezi University, Xinjiang, 832003, PR CHINAPCR single-strand conformation polymorphism (SSCP) and DNA sequencing techniques were used to analyze the genetic polymorphism of mannose-binding lectin (MBL) gene 3 introns in Hu sheep. The results showed that 3 introns of MBL gene had polymorphism, 3 genotypes were identified in intron1, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron2, which were controlled by 2 alleles, respectively; 3 genotypes were identified in intron3, which were controlled by 2 alleles, respectively; 6 new single nucleotide polymorphisms(SNPs) mutation sites were found, g.288T>A in intron1, g. 1091 T>C, g.1096A>C, g.1770G>C in intron 2, g.2297C>T, g.2331G>A in intron3. Use ELISA to detect MBL serum level in Hu sheep, then use One-way ANOVA analyze the relationship of different genotypes and MBL serum level. And the result showed that the MBL serum level of AA genotype was higher than BB genotype (P<0.05) in intron1, CC genotype was higher than DD, GG genotype was higher than HH (P<0.05) in intron2. So, AA, CC and GG genotypes may be related to disease resistance; BB, DD and HH genotypes be related to disease susceptibility. The point mutation in intron3 couldn"t lead to the change of MBL serum level.https://vetdergikafkas.org/pdf.php?id=2481sheepmblmycoplasma pneumoniapcr-sscpelisaintrons |
| spellingShingle | Mengting ZHAI Jian MOU Mengting ZHU Yanping LIANG Mingyuan WANG Zongsheng ZHAO Hongmei ZHANG Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep Kafkas Universitesi Veteriner Fakültesi Dergisi sheep mbl mycoplasma pneumonia pcr-sscp elisa introns |
| title | Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| title_full | Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| title_fullStr | Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| title_full_unstemmed | Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| title_short | Polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| title_sort | polymorphisms of mbl gene introns and their association with mbl serum levels in hu sheep |
| topic | sheep mbl mycoplasma pneumonia pcr-sscp elisa introns |
| url | https://vetdergikafkas.org/pdf.php?id=2481 |
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