Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection
Optical fiber sensors, thanks to their compactness, fast response and real-time measurements, have a large impact in the fields of life science research, drug discovery and medical diagnostics. In recent years, advances in nanotechnology have resulted in the development of nanotools, capable of ente...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-04-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/15/5/9666 |
| _version_ | 1798025874407161856 |
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| author | Ambra Giannetti Andrea Barucci Franco Cosi Stefano Pelli Sara Tombelli Cosimo Trono Francesco Baldini |
| author_facet | Ambra Giannetti Andrea Barucci Franco Cosi Stefano Pelli Sara Tombelli Cosimo Trono Francesco Baldini |
| author_sort | Ambra Giannetti |
| collection | DOAJ |
| description | Optical fiber sensors, thanks to their compactness, fast response and real-time measurements, have a large impact in the fields of life science research, drug discovery and medical diagnostics. In recent years, advances in nanotechnology have resulted in the development of nanotools, capable of entering the single cell, resulting in new nanobiosensors useful for the detection of biomolecules inside living cells. In this paper, we provide an application of a nanotip coupled with molecular beacons (MBs) for the detection of DNA. The MBs were characterized by hybridization studies with a complementary target to prove their functionality both free in solution and immobilized onto a solid support. The solid support chosen as substrate for the immobilization of the MBs was a 30 nm tapered tip of an optical fiber, fabricated by chemical etching. With this set-up promising results were obtained and a limit of detection (LOD) of 0.57 nM was reached, opening up the possibility of using the proposed nanotip to detect mRNAs inside the cytoplasm of living cells. |
| first_indexed | 2024-04-11T18:25:57Z |
| format | Article |
| id | doaj.art-fc390b195e8d4fee9033b3c35e111775 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:25:57Z |
| publishDate | 2015-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-fc390b195e8d4fee9033b3c35e1117752022-12-22T04:09:38ZengMDPI AGSensors1424-82202015-04-011559666968010.3390/s150509666s150509666Optical Fiber Nanotips Coated with Molecular Beacons for DNA DetectionAmbra Giannetti0Andrea Barucci1Franco Cosi2Stefano Pelli3Sara Tombelli4Cosimo Trono5Francesco Baldini6CNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyCNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), ItalyOptical fiber sensors, thanks to their compactness, fast response and real-time measurements, have a large impact in the fields of life science research, drug discovery and medical diagnostics. In recent years, advances in nanotechnology have resulted in the development of nanotools, capable of entering the single cell, resulting in new nanobiosensors useful for the detection of biomolecules inside living cells. In this paper, we provide an application of a nanotip coupled with molecular beacons (MBs) for the detection of DNA. The MBs were characterized by hybridization studies with a complementary target to prove their functionality both free in solution and immobilized onto a solid support. The solid support chosen as substrate for the immobilization of the MBs was a 30 nm tapered tip of an optical fiber, fabricated by chemical etching. With this set-up promising results were obtained and a limit of detection (LOD) of 0.57 nM was reached, opening up the possibility of using the proposed nanotip to detect mRNAs inside the cytoplasm of living cells.http://www.mdpi.com/1424-8220/15/5/9666molecular beaconoptical fiber nanotipnanosensoroptical biosensorsfluorescencemRNADNAsurvivin |
| spellingShingle | Ambra Giannetti Andrea Barucci Franco Cosi Stefano Pelli Sara Tombelli Cosimo Trono Francesco Baldini Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection Sensors molecular beacon optical fiber nanotip nanosensor optical biosensors fluorescence mRNA DNA survivin |
| title | Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection |
| title_full | Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection |
| title_fullStr | Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection |
| title_full_unstemmed | Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection |
| title_short | Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection |
| title_sort | optical fiber nanotips coated with molecular beacons for dna detection |
| topic | molecular beacon optical fiber nanotip nanosensor optical biosensors fluorescence mRNA DNA survivin |
| url | http://www.mdpi.com/1424-8220/15/5/9666 |
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