An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber
Abstract Background The release of organic acids (OAs) is considered the main mechanism used by phosphate-solubilizing bacteria (PSB) to dissolve inorganic phosphate in soil. Nevertheless, little is known about the effect of individual OAs produced by a particular PSB in a soil–plant system. For the...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02918-6 |
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| author | Mónica Yorlady Alzate Zuluaga André Luiz Martinez de Oliveira Fabio Valentinuzzi Nádia Souza Jayme Sonia Monterisi Roberto Fattorini Stefano Cesco Youry Pii |
| author_facet | Mónica Yorlady Alzate Zuluaga André Luiz Martinez de Oliveira Fabio Valentinuzzi Nádia Souza Jayme Sonia Monterisi Roberto Fattorini Stefano Cesco Youry Pii |
| author_sort | Mónica Yorlady Alzate Zuluaga |
| collection | DOAJ |
| description | Abstract Background The release of organic acids (OAs) is considered the main mechanism used by phosphate-solubilizing bacteria (PSB) to dissolve inorganic phosphate in soil. Nevertheless, little is known about the effect of individual OAs produced by a particular PSB in a soil–plant system. For these reasons, the present work aimed at investigating the effect of Enterobacter sp. strain 15S and the exogenous application of its OAs on (i) the solubilization of tricalcium phosphate (TCP), (ii) plant growth and (iii) P nutrition of cucumber. To this purpose two independent experiments have been performed. Results In the first experiment, carried out in vitro, the phosphate solubilizing activity of Enterobacter 15S was associated with the release of citric, fumaric, ketoglutaric, malic, and oxalic acids. In the second experiment, cucumber plants were grown in a Leonard jar system consisting of a nutrient solution supplemented with the OAs previously identified in Enterobacter 15S (jar’s base) and a substrate supplemented with the insoluble TCP where cucumber plants were grown (jar’s top). The use of Enterobacter 15S and its secreted OAs proved to be efficient in the in situ TCP solubilization. In particular, the enhancement of the morpho-physiological traits of P-starved cucumber plants was evident when treated with Enterobacter 15S, oxalate, or citrate. The highest accumulation of P in roots and shoots induced by such treatments further corroborated this hypothesis. Conclusion In our study, the results presented suggest that organic acids released by Enterobacter 15S as well as the bacterium itself can enhance the P-acquisition by cucumber plants. |
| first_indexed | 2024-03-12T23:25:45Z |
| format | Article |
| id | doaj.art-fc39aa306c144077b6bd8f2346d2701a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-12T23:25:45Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-fc39aa306c144077b6bd8f2346d2701a2023-07-16T11:11:08ZengBMCBMC Microbiology1471-21802023-07-0123111610.1186/s12866-023-02918-6An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumberMónica Yorlady Alzate Zuluaga0André Luiz Martinez de Oliveira1Fabio Valentinuzzi2Nádia Souza Jayme3Sonia Monterisi4Roberto Fattorini5Stefano Cesco6Youry Pii7Faculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoDepartment of Biochemistry and Biotechnology, State University of LondrinaFaculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoDepartment of Biochemistry and Biotechnology, State University of LondrinaFaculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoFaculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoFaculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoFaculty of Agricultural, Environmental and Food Sciences, Free University of BolzanoAbstract Background The release of organic acids (OAs) is considered the main mechanism used by phosphate-solubilizing bacteria (PSB) to dissolve inorganic phosphate in soil. Nevertheless, little is known about the effect of individual OAs produced by a particular PSB in a soil–plant system. For these reasons, the present work aimed at investigating the effect of Enterobacter sp. strain 15S and the exogenous application of its OAs on (i) the solubilization of tricalcium phosphate (TCP), (ii) plant growth and (iii) P nutrition of cucumber. To this purpose two independent experiments have been performed. Results In the first experiment, carried out in vitro, the phosphate solubilizing activity of Enterobacter 15S was associated with the release of citric, fumaric, ketoglutaric, malic, and oxalic acids. In the second experiment, cucumber plants were grown in a Leonard jar system consisting of a nutrient solution supplemented with the OAs previously identified in Enterobacter 15S (jar’s base) and a substrate supplemented with the insoluble TCP where cucumber plants were grown (jar’s top). The use of Enterobacter 15S and its secreted OAs proved to be efficient in the in situ TCP solubilization. In particular, the enhancement of the morpho-physiological traits of P-starved cucumber plants was evident when treated with Enterobacter 15S, oxalate, or citrate. The highest accumulation of P in roots and shoots induced by such treatments further corroborated this hypothesis. Conclusion In our study, the results presented suggest that organic acids released by Enterobacter 15S as well as the bacterium itself can enhance the P-acquisition by cucumber plants.https://doi.org/10.1186/s12866-023-02918-6Phosphate solubilizationPhosphate-solubilizing bacteria (PSB)Enterobacter sp.Organic acidsP-use efficiency |
| spellingShingle | Mónica Yorlady Alzate Zuluaga André Luiz Martinez de Oliveira Fabio Valentinuzzi Nádia Souza Jayme Sonia Monterisi Roberto Fattorini Stefano Cesco Youry Pii An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber BMC Microbiology Phosphate solubilization Phosphate-solubilizing bacteria (PSB) Enterobacter sp. Organic acids P-use efficiency |
| title | An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber |
| title_full | An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber |
| title_fullStr | An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber |
| title_full_unstemmed | An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber |
| title_short | An insight into the role of the organic acids produced by Enterobacter sp. strain 15S in solubilizing tricalcium phosphate: in situ study on cucumber |
| title_sort | insight into the role of the organic acids produced by enterobacter sp strain 15s in solubilizing tricalcium phosphate in situ study on cucumber |
| topic | Phosphate solubilization Phosphate-solubilizing bacteria (PSB) Enterobacter sp. Organic acids P-use efficiency |
| url | https://doi.org/10.1186/s12866-023-02918-6 |
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