Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures
Background: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaini...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/12/2350 |
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| author | Yulia Kononova Lyubov Adamenko Evgeniya Kazachkova Mariya Solomatina Svetlana Romanenko Anastasia Proskuryakova Yaroslav Utkin Marina Gulyaeva Anastasia Spirina Elena Kazachinskaia Natalia Palyanova Oksana Mishchenko Alexander Chepurnov Alexander Shestopalov |
| author_facet | Yulia Kononova Lyubov Adamenko Evgeniya Kazachkova Mariya Solomatina Svetlana Romanenko Anastasia Proskuryakova Yaroslav Utkin Marina Gulyaeva Anastasia Spirina Elena Kazachinskaia Natalia Palyanova Oksana Mishchenko Alexander Chepurnov Alexander Shestopalov |
| author_sort | Yulia Kononova |
| collection | DOAJ |
| description | Background: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaining its circulation remains open. Methods: the aim of this work was to study the tropism of SARS-CoV-2 for cells of freshwater fish and reptiles, including those associated with aquatic and coastal ecosystems, and the effect of ambient temperature on this process. In a continuous cell culture FHM (fathead minnow) and diploid fibroblasts CGIB (silver carp), SARS-CoV-2 replication was not maintained at either 25 °C or 29 °C. At 29 °C, the continuous cell culture TH-1 (eastern box turtle) showed high susceptibility to SARS-CoV-2, comparable to Vero E6 (development of virus-induced cytopathic effect (CPE) and an infectious titer of 7.5 ± 0.17 log<sub>10</sub> TCID<sub>50</sub>/mL on day 3 after infection), and primary fibroblasts CNI (Nile crocodile embryo) showed moderate susceptibility (no CPE, infectious titer 4.52 ± 0.14 log<sub>10</sub> TCID<sub>50</sub>/mL on day 5 after infection). At 25 °C, SARS-CoV-2 infection did not develop in TH-1 and CNI. Conclusions: our results show the ability of SARS-CoV-2 to effectively replicate without adaptation in the cells of certain reptile species when the ambient temperature rises. |
| first_indexed | 2024-03-08T20:17:41Z |
| format | Article |
| id | doaj.art-fc3cc6110c274071bda85f6ec066624c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T20:17:41Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fc3cc6110c274071bda85f6ec066624c2023-12-22T14:49:10ZengMDPI AGViruses1999-49152023-11-011512235010.3390/v15122350Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell CulturesYulia Kononova0Lyubov Adamenko1Evgeniya Kazachkova2Mariya Solomatina3Svetlana Romanenko4Anastasia Proskuryakova5Yaroslav Utkin6Marina Gulyaeva7Anastasia Spirina8Elena Kazachinskaia9Natalia Palyanova10Oksana Mishchenko11Alexander Chepurnov12Alexander Shestopalov13Federal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaInstitute of Molecular and Cellular Biology, Russian Academy of Sciences, Siberian Branch, Novosibirsk 630090, RussiaInstitute of Molecular and Cellular Biology, Russian Academy of Sciences, Siberian Branch, Novosibirsk 630090, RussiaInstitute of Molecular and Cellular Biology, Russian Academy of Sciences, Siberian Branch, Novosibirsk 630090, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, Russia48 Central Research Institute of the Ministry of Defense of the Russian Federation, Moscow 141306, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaFederal Research Center of Fundamental and Translational Medicine, The Federal State Budget Scientific Institution, Siberian Branch of the Russian Academy of Sciences, 2, Timakova St., Novosibirsk 630117, RussiaBackground: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaining its circulation remains open. Methods: the aim of this work was to study the tropism of SARS-CoV-2 for cells of freshwater fish and reptiles, including those associated with aquatic and coastal ecosystems, and the effect of ambient temperature on this process. In a continuous cell culture FHM (fathead minnow) and diploid fibroblasts CGIB (silver carp), SARS-CoV-2 replication was not maintained at either 25 °C or 29 °C. At 29 °C, the continuous cell culture TH-1 (eastern box turtle) showed high susceptibility to SARS-CoV-2, comparable to Vero E6 (development of virus-induced cytopathic effect (CPE) and an infectious titer of 7.5 ± 0.17 log<sub>10</sub> TCID<sub>50</sub>/mL on day 3 after infection), and primary fibroblasts CNI (Nile crocodile embryo) showed moderate susceptibility (no CPE, infectious titer 4.52 ± 0.14 log<sub>10</sub> TCID<sub>50</sub>/mL on day 5 after infection). At 25 °C, SARS-CoV-2 infection did not develop in TH-1 and CNI. Conclusions: our results show the ability of SARS-CoV-2 to effectively replicate without adaptation in the cells of certain reptile species when the ambient temperature rises.https://www.mdpi.com/1999-4915/15/12/2350SARS-CoV-2aquatic ecosystemsfish cellsreptile cellsTH-1 cell culture |
| spellingShingle | Yulia Kononova Lyubov Adamenko Evgeniya Kazachkova Mariya Solomatina Svetlana Romanenko Anastasia Proskuryakova Yaroslav Utkin Marina Gulyaeva Anastasia Spirina Elena Kazachinskaia Natalia Palyanova Oksana Mishchenko Alexander Chepurnov Alexander Shestopalov Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures Viruses SARS-CoV-2 aquatic ecosystems fish cells reptile cells TH-1 cell culture |
| title | Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures |
| title_full | Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures |
| title_fullStr | Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures |
| title_full_unstemmed | Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures |
| title_short | Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures |
| title_sort | features of sars cov 2 replication in various types of reptilian and fish cell cultures |
| topic | SARS-CoV-2 aquatic ecosystems fish cells reptile cells TH-1 cell culture |
| url | https://www.mdpi.com/1999-4915/15/12/2350 |
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