Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma
Cistanche deserticola Y. C. Ma, a precious parasitic medicinal herb distributed in desert areas in the Northwest of China, also known as “desert ginseng”, has been used in China for thousands of years for its nourishing effects. The phenylethanoid glycosides (PeGs) have been proven as the main effec...
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MDPI AG
2018-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/4/893 |
| _version_ | 1818257365169864704 |
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| author | Gaosheng Hu Tianran Wu Yue Chang Xinyi Zhan Jingming Jia |
| author_facet | Gaosheng Hu Tianran Wu Yue Chang Xinyi Zhan Jingming Jia |
| author_sort | Gaosheng Hu |
| collection | DOAJ |
| description | Cistanche deserticola Y. C. Ma, a precious parasitic medicinal herb distributed in desert areas in the Northwest of China, also known as “desert ginseng”, has been used in China for thousands of years for its nourishing effects. The phenylethanoid glycosides (PeGs) have been proven as the main effective compounds due to their neuroprotective effects and were used for quality control. In this study, echinacoside content, a representative PeG, total phenolic content, DPPH scavenging activity, and PAL activity were determined in different tissues of C. deserticola. Our results showed that most indices had a similar pattern of scale > cambium ring > pith and bottom part > middle part > upper part. Besides, stereomicroscopic observation showed that the scale surface was densely covered with physical wounds formed during vertical and broadwise growth in sand. Thus, wound area was quantified and a linear regression analysis was conducted between wound area and PAL activity, total phenolics, and echinacoside content. Our results suggested that physical wounding caused by sand might play an important role in echinacoside biosynthesis which has never been noticed in C. deserticola development. Furthermore, the coexistence of the highest PAL activity and highest echinacoside accumulation in scale tissue might indicate that the biosynthetic site of echinacoside in C. deseticola Y. C. Ma is mainly in the scale tissue. |
| first_indexed | 2024-12-12T17:42:29Z |
| format | Article |
| id | doaj.art-fc3e02543f0e412b97839f904b1770f0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T17:42:29Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fc3e02543f0e412b97839f904b1770f02022-12-22T00:17:01ZengMDPI AGMolecules1420-30492018-04-0123489310.3390/molecules23040893molecules23040893Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. MaGaosheng Hu0Tianran Wu1Yue Chang2Xinyi Zhan3Jingming Jia4School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaCistanche deserticola Y. C. Ma, a precious parasitic medicinal herb distributed in desert areas in the Northwest of China, also known as “desert ginseng”, has been used in China for thousands of years for its nourishing effects. The phenylethanoid glycosides (PeGs) have been proven as the main effective compounds due to their neuroprotective effects and were used for quality control. In this study, echinacoside content, a representative PeG, total phenolic content, DPPH scavenging activity, and PAL activity were determined in different tissues of C. deserticola. Our results showed that most indices had a similar pattern of scale > cambium ring > pith and bottom part > middle part > upper part. Besides, stereomicroscopic observation showed that the scale surface was densely covered with physical wounds formed during vertical and broadwise growth in sand. Thus, wound area was quantified and a linear regression analysis was conducted between wound area and PAL activity, total phenolics, and echinacoside content. Our results suggested that physical wounding caused by sand might play an important role in echinacoside biosynthesis which has never been noticed in C. deserticola development. Furthermore, the coexistence of the highest PAL activity and highest echinacoside accumulation in scale tissue might indicate that the biosynthetic site of echinacoside in C. deseticola Y. C. Ma is mainly in the scale tissue.http://www.mdpi.com/1420-3049/23/4/893biosynthesisCistanche deserticola Y. C. Maechinacosidephenylalanine ammonia lyasephysical wounding |
| spellingShingle | Gaosheng Hu Tianran Wu Yue Chang Xinyi Zhan Jingming Jia Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma Molecules biosynthesis Cistanche deserticola Y. C. Ma echinacoside phenylalanine ammonia lyase physical wounding |
| title | Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma |
| title_full | Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma |
| title_fullStr | Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma |
| title_full_unstemmed | Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma |
| title_short | Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma |
| title_sort | wound stress an unheeded factor for echinacoside accumulation in cistanche deserticola y c ma |
| topic | biosynthesis Cistanche deserticola Y. C. Ma echinacoside phenylalanine ammonia lyase physical wounding |
| url | http://www.mdpi.com/1420-3049/23/4/893 |
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