Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota
In broiler chicken production, microbial populations on the eggshell surface following oviposition are still poorly characterized, though they may significantly impact both poultry and public health. The aim of this study was to describe the microbiota of both broiler breeder hens’ feces and the sur...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00666/full |
| _version_ | 1818410449030348800 |
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| author | Sandrine Trudeau Alexandre Thibodeau Alexandre Thibodeau Jean-Charles Côté Marie-Lou Gaucher Marie-Lou Gaucher Philippe Fravalo Philippe Fravalo Philippe Fravalo |
| author_facet | Sandrine Trudeau Alexandre Thibodeau Alexandre Thibodeau Jean-Charles Côté Marie-Lou Gaucher Marie-Lou Gaucher Philippe Fravalo Philippe Fravalo Philippe Fravalo |
| author_sort | Sandrine Trudeau |
| collection | DOAJ |
| description | In broiler chicken production, microbial populations on the eggshell surface following oviposition are still poorly characterized, though they may significantly impact both poultry and public health. The aim of this study was to describe the microbiota of both broiler breeder hens’ feces and the surface of their eggs to assess the contribution of the parental fecal microbiota to the eggshell microbiota. A total of twelve breeder flocks in Quebec, Canada, were sampled at two different times, and a total of 940 feces and 16,400 egg surface samples were recovered. Using 16S rRNA gene sequencing, we showed that even if the microbiota of both feces and eggshells were mainly composed of the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes, the bacterial community compositions and structures differed between both types of samples. Our results also showed that both the sampling time and the flock identity significantly influenced the alpha- and the beta-diversities of the studied microbiomes. Using a Venn diagram, we showed that 1790 operational taxonomic units (OTUs) were shared between feces and eggshell samples. Sequences associated with genera of potentially pathogenic and spoilage bacteria, Acinetobacter, Campylobacter, Escherichia/Shigella, Helicobacter, Listeria, Proteus, Pseudomonas, Salmonella, and Staphylococcus, were shared between sample types. Some OTUs highly represented in the fecal microbiota and associated with Lactobacillus and Streptococcus genera, were absent from eggshells, suggesting a selection during the microbiota transfer and/or the potential role of environmental contamination. To the best of our knowledge, this is the first study using 16S rRNA sequencing to describe the contribution of the transfer from the fecal microbial ecosystem of laying breeder hens to the establishment of the microbiota on the surface of laid eggs, as well as the bacterial communities at both the broiler breeder feces and the eggshell levels. |
| first_indexed | 2024-12-14T10:15:41Z |
| format | Article |
| id | doaj.art-fc3fdff81d6843d2aa30f2b6b0edaef7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T10:15:41Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fc3fdff81d6843d2aa30f2b6b0edaef72022-12-21T23:06:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-04-011110.3389/fmicb.2020.00666519157Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell MicrobiotaSandrine Trudeau0Alexandre Thibodeau1Alexandre Thibodeau2Jean-Charles Côté3Marie-Lou Gaucher4Marie-Lou Gaucher5Philippe Fravalo6Philippe Fravalo7Philippe Fravalo8NSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaCRIPA Swine and Poultry Infectious Diseases Research Center, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaCRIPA Swine and Poultry Infectious Diseases Research Center, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaNSERC Industrial Research Chair in Meat Safety (CRSV), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaCRIPA Swine and Poultry Infectious Diseases Research Center, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, CanadaPôle Agroalimentaire, Conservatoire National des Arts et Métiers (Cnam), Paris, FranceIn broiler chicken production, microbial populations on the eggshell surface following oviposition are still poorly characterized, though they may significantly impact both poultry and public health. The aim of this study was to describe the microbiota of both broiler breeder hens’ feces and the surface of their eggs to assess the contribution of the parental fecal microbiota to the eggshell microbiota. A total of twelve breeder flocks in Quebec, Canada, were sampled at two different times, and a total of 940 feces and 16,400 egg surface samples were recovered. Using 16S rRNA gene sequencing, we showed that even if the microbiota of both feces and eggshells were mainly composed of the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes, the bacterial community compositions and structures differed between both types of samples. Our results also showed that both the sampling time and the flock identity significantly influenced the alpha- and the beta-diversities of the studied microbiomes. Using a Venn diagram, we showed that 1790 operational taxonomic units (OTUs) were shared between feces and eggshell samples. Sequences associated with genera of potentially pathogenic and spoilage bacteria, Acinetobacter, Campylobacter, Escherichia/Shigella, Helicobacter, Listeria, Proteus, Pseudomonas, Salmonella, and Staphylococcus, were shared between sample types. Some OTUs highly represented in the fecal microbiota and associated with Lactobacillus and Streptococcus genera, were absent from eggshells, suggesting a selection during the microbiota transfer and/or the potential role of environmental contamination. To the best of our knowledge, this is the first study using 16S rRNA sequencing to describe the contribution of the transfer from the fecal microbial ecosystem of laying breeder hens to the establishment of the microbiota on the surface of laid eggs, as well as the bacterial communities at both the broiler breeder feces and the eggshell levels.https://www.frontiersin.org/article/10.3389/fmicb.2020.00666/full16S rRNAanimal healthbacterial transferbroiler breederseggshell microbiotafecal microbiota |
| spellingShingle | Sandrine Trudeau Alexandre Thibodeau Alexandre Thibodeau Jean-Charles Côté Marie-Lou Gaucher Marie-Lou Gaucher Philippe Fravalo Philippe Fravalo Philippe Fravalo Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota Frontiers in Microbiology 16S rRNA animal health bacterial transfer broiler breeders eggshell microbiota fecal microbiota |
| title | Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota |
| title_full | Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota |
| title_fullStr | Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota |
| title_full_unstemmed | Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota |
| title_short | Contribution of the Broiler Breeders’ Fecal Microbiota to the Establishment of the Eggshell Microbiota |
| title_sort | contribution of the broiler breeders fecal microbiota to the establishment of the eggshell microbiota |
| topic | 16S rRNA animal health bacterial transfer broiler breeders eggshell microbiota fecal microbiota |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00666/full |
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