Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics
Second-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor eff...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/995 |
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| author | Bruno Weiss Anna Carolina Oliveira Souza Milena Tavares Lima Constancio Danillo Oliveira Alvarenga Victor S. Pylro Lucia M. Carareto Alves Alessandro M. Varani |
| author_facet | Bruno Weiss Anna Carolina Oliveira Souza Milena Tavares Lima Constancio Danillo Oliveira Alvarenga Victor S. Pylro Lucia M. Carareto Alves Alessandro M. Varani |
| author_sort | Bruno Weiss |
| collection | DOAJ |
| description | Second-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., <i>Chryseobacterium</i>) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., <i>Chitinophaga</i>, <i>Niastella</i>, <i>Niabella</i>, and <i>Siphonobacter</i>). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium. |
| first_indexed | 2024-03-10T11:41:57Z |
| format | Article |
| id | doaj.art-fc420233fd154866aa034b073c3f6a84 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:41:57Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fc420233fd154866aa034b073c3f6a842023-11-21T18:25:25ZengMDPI AGMicroorganisms2076-26072021-05-019599510.3390/microorganisms9050995Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered MetagenomicsBruno Weiss0Anna Carolina Oliveira Souza1Milena Tavares Lima Constancio2Danillo Oliveira Alvarenga3Victor S. Pylro4Lucia M. Carareto Alves5Alessandro M. Varani6Departament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilDepartament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilDepartament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilDepartament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilMicrobial Ecology and Bioinformatics Laboratory, Department of Biology, Federal University of Lavras (UFLA), Lavras, Minas Gerais 37200-000, BrazilDepartament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilDepartament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, BrazilSecond-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., <i>Chryseobacterium</i>) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., <i>Chitinophaga</i>, <i>Niastella</i>, <i>Niabella</i>, and <i>Siphonobacter</i>). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium.https://www.mdpi.com/2076-2607/9/5/995lignocellulosebiotechnologymetabolic modelingbiofuelscommunity dynamics |
| spellingShingle | Bruno Weiss Anna Carolina Oliveira Souza Milena Tavares Lima Constancio Danillo Oliveira Alvarenga Victor S. Pylro Lucia M. Carareto Alves Alessandro M. Varani Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics Microorganisms lignocellulose biotechnology metabolic modeling biofuels community dynamics |
| title | Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics |
| title_full | Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics |
| title_fullStr | Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics |
| title_full_unstemmed | Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics |
| title_short | Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics |
| title_sort | unraveling a lignocellulose decomposing bacterial consortium from soil associated with dry sugarcane straw by genomic centered metagenomics |
| topic | lignocellulose biotechnology metabolic modeling biofuels community dynamics |
| url | https://www.mdpi.com/2076-2607/9/5/995 |
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